Both subunits of ATP-citrate lyase from Chlorobium tepidum contribute to catalytic activity

Journal of Bacteriology

Volumn 188, Issue 18, 2006, Pages 6544-6552

  Kim, Wonduck ^a   Tabita, F Robert ^a  

^a OHIO STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYL COENZYME A; ADENOSINE TRIPHOSPHATE CITRATE LYASE; ADENOSINE TRIPHOSPHATE MAGNESIUM; AMINO ACID; CARBON DIOXIDE; CITRIC ACID; HOLOENZYME; OXALOACETIC ACID;

ARTICLE; CATALYSIS; CHLOROBI; CHLOROBIUM TEPIDUM; ENZYME SUBUNIT; ESCHERICHIA COLI; GENE EXPRESSION; MUTAGENESIS; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROKARYOTE; STOICHIOMETRY;

AMINO ACID SEQUENCE; ATP CITRATE (PRO-S)-LYASE; CATALYSIS; CHLOROBIUM; CITRIC ACID; CLONING, MOLECULAR; DNA MUTATIONAL ANALYSIS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTAGENESIS, SITE-DIRECTED; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

BACTERIA (MICROORGANISMS); CHLOROBACULUM TEPIDUM; CHLOROBI; ESCHERICHIA COLI; PROKARYOTA;

EID: 33748780101     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.00523-06     Document Type: Article

References (35)

1. Atomi, H. 2002, Microbial enzymes involved in carbon dioxide fixation. J. Biosci. Bioeng. 94:497-505.
2. Beh, M., G. Strauss, R. Huber, K. O. Stetter, and G. Fuchs. 1993. Enzymes of the reductive citric acid cycle in the autotrophic eubacterium Aquifex pyrophilus and in the archaebacterium Thermoproteus neutrophilus. Arch. Microbiol. 160:306-311.
3. Eisen, J. A., K. E. Nelson, I. T. Paulsen, J. F. Heidelberg, M. Wu, R. J. Dodson, R. Deboy, M. L. Gwinn, W. C. Nelson, D. H. Haft, E. K. Hickey, J. D. Peterson, A. S. Durkin, J. L. Kolonay, F. Yang, I. Holt, L. A. Umayam, T. Mason, M. Brenner, T. P. Shea, D. Parksey, W. C. Nierman, T. V. Feldblyum, C. L. Hansen, M. B. Craven, D. Radune, J. Vamathevan, H. Khouri, O. White, T. M. Gruber, K. A. Ketchum, J. C. Venter, H. Tettelin, D. A. Bryant, and C. M. Fraser. 2002. The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium. Proc. Natl. Acad. Sci. USA 99:9509-9514.
4. Elshourbagy, N. A., J. C. Near, P. J. Kmetz, G. M. Sathe, C. Southan, J. E. Strickler, M. Gross, J. F. Young, T. N. C. Wells, and P. H. E. Groot. 1990. Rat ATP citrate-lyase. Molecular cloning and sequence analysis of a full-length cDNA and mRNA abundance as a function of diet, organ, and age. J. Biol. Chem. 265:1430-1435.
5. Engel, C., and R. Wierenga. 1996. The diverse world of coenzyme A binding proteins. Curr. Opin. Struct. Biol. 6:790-797.
6. Evans, M. C. W., B. B. Buchanan, and D. I. Arnon. 1966. A new ferredoxin-dependent carbon reduction cycle in a photosynthetic bacterium. Proc. Natl. Acad. Sci. USA 55:928-934.
7. Fraser, M. E., M. N. G. James, W. A. Bridger, and W. T. Wolodko. 1999. A detailed structural description of Escherichia coli succinyl-CoA synthetase. J. Mol. Biol. 285:1633-1653.
8. Fraser, M. E., M. A. Joyce, D. G. Ryan, and W. T. Wolodko. 2002, Two glutamate residues, Glu 208α and Glu 197β, are crucial for phosphorylation and dephosphorylation of the active-site histidine residue in succinyl-CoA synthetase. Biochemistry 41:537-546.
9. 2 fixation in Chlorobium limicola. Evidence for the operation of a reductive tricarboxylic acid cycle in growing cells. Arch. Microbiol. 128:64-71.
10. Galperin, M. Y., and E. V. Koonin. 1997. A diverse superfamily of enzymes with ATP dependent carboxylate-amine/thiol ligase activity. Protein Sci. 6:2639-2643.
11. 2 fixation pathways in archaea (Crenarchaeota). Arch. Microbiol. 179:160-173.
12. Hügler, M., C. O. Wirsen, G. Fuchs, C. D. Taylor, and S. M. Sievert. 2005. Evidence for autotrophic CO2 fixation via the reductive tricarboxylic acid cycle by members of the ε subdivision of proteobacteria. J. Bacteriol. 187:3020-3027.
13. Ivanovsky, R. N., N. V. Sintsov, and E. N. Konsratieva. 1980. ATP-linked citrate lyase activity in the green sulfur bacterium Chlorobium limicola forma thiosidfatophilum. Arch. Microbiol, 128:239-241.
14. Joyce, M. A., M. E. Fraser, E. R. Brownie, M. N. G. James, W. A. Bridger, and W. T. Wolodko. 1999. Probing the nucleotide-binding site of Escherichia coli succinyl-CoA synthetase. Biochemistry 38:7273-7283.
15. Joyce, M. A., M. E. Fraser, M. N. G. James, W. A. Bridger, and W. T. Wolodko. 2000. ADP-binding site of Escherichia coli succinyl-CoA synthetase revealed by X-ray crystallography. Biochemistry 39:17-25.
16. Kanao, T., T. Fukui, H. Atomi, and T. Imanaka. 2001. ATP-citrate lyase from the green sulfur bacterium Chlorobium limicola is a heteromeric enzyme composed of two distinct gene products. Eur. J. Biochem. 268:1670-1678.
17. Kanao, T., T. Fukui, H. Atomi, and T. Imanaka. 2002. Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase. Eur. J. Biochem. 269:3409-3416.
18. Linn, T. C., and P. A. Srere. 1979, Identification of ATP citrate lyase as a phosphoprotein. J. Biol. Chem. 254:1691-1698.
19. Pentyala, S. N., and W. B. Benjamin. 1995. Effect of oxaloacetate and phosphoiylation on ATP-citrate lyase activity. Biochemistry 34:10961-10969.
20. Pereira, D. S., L. J. Donald, D. J. Hosfield, and H. W. Duckworth. 1994. Active site mutants of Escherichia coli citrate synthase. Effects of mutations on catalytic and allosteric properties. J. Biol. Chem, 269:412-417.
21. Pierce, M. W., J. L. Palmer, H. T. Keutmann, and J. Avrnch. 1981. ATP-citrate lyase, Structure of a tryptic peptide containing the phosphorylation site directed by glucagon and the cAMP-dependent protein kinase. J. Biol. Chem. 256:8867-3870,
22. Potapova, I. A., M. R. El-Maghrabi, S. V. Doronin, and W. B. Benjamin. 2000. Phosphorylation of recombinant human ATP: citrate lyase by cAMP-dependent protein kinase abolishes homotropic allosteric regulation of the enzyme by citrate and increases the enzyme activity, Allosteric activation of ATP:citrate lyase by phosphorylated sugars. Biochemistry 39:1169-1179.
23. Ramakrishna, S., G. D'Angleo, and W. B. Benjamin. 1990. Sequence of sites on ATP-citrate lyase and phosphatase inhibitor 2 phosphorylated by multifunctional protein kinase (a glycogen synthase kinase 3 like kinase). Biochemistry 29:7617-7624.
24. Sambrook, J., and D. W. Russell. 2003. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
25. Sato, R., A. Okamoto, J. Inoue, W. Miyamoto, Y. Sakai, N. Emoto, H. Shimano, and M. Maeda. 2000. Transcriptional regulation of the ATP citrate-lyase gene by sterol regulatory element-binding proteins. J. Biol. Chem. 275:12497-12502.
26. Schäfer, S., C. Barkowski, and G. Fuchs. 1986. Carbon assimilation by the autotrophic thermophilic archaebacterium Thermoproteus neutrophilus. Arh. Microbiol. 146:301-308.
27. Shauder, R., F. Widdel, and G. Fuchs. 1987. Carbon assimilation pathways in sulfate-reducing bacteria. II. Enzymes of a reductive citric acid cycle in the autotrophic Desulfobacter thermophilus TK-6. Arch. Microbiol. 148:218-225.
28. 2 assimilation via the reductive tricarboxylic acid cycle in an obligately autotrophic, aerobic hydrogen oxidizing bacterium, Hydrogenobacter thermophilus. Arch. Microbiol. 141:198-203.
29. Singh, M., E. G. Richards, A. Mukherjee, and P. A. Srere. 1976. Structure of ATP citrate lyase from rat liver. Physicochemical studies and proteolytic modification. J. Biol. Chem. 251:5242-5250.
30. Srere, P. A. 1959. The citrate cleavage enzyme. I, Distribution and purification. J. Biol. Chem. 234:2544-2547.
31. Studier, F. W., and B. A. Moffatt. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. MoI. Biol. 189:113-130.
32. Wahlund, T. M., and F. R. Tabita. 1997. The reductive tricarboxylic acid cycle of carbon dioxide assimilation: initial studies and purification of ATP-citrate lyase form the green sulfur bacterium Chlorobium tepidum. J. Bacteriol. 179:4859-4867.
33. Walsh, C. T. 1979. Enzymatic reaction mechanisms. W.H. Freeman & Co., New York, N.Y.
34. Wells, T. N. C. 1991. ATP-citrate lyase from rat liver. Characterization of the citryl-enzyme complexes. Eur. J. Biochem. 199:163-168.
35. Wolodko, W. T., M. E. Fraser, M. N. G. James, and W. A. Bridger. 1994. The crystal structure of succinyl-CoA synthetase from Escherichia coli at 2.5-Å resolution. J. Biol. Chem. 269:10883-10890.