Development and validation of LC-MS/MS method for determination of very long acyl chain (C22:0 and C24:0) ceramides in human plasma

Analytical and Bioanalytical Chemistry

Volumn 405, Issue 23, 2013, Pages 7357-7365

  Jiang, Hui ^a   Hsu, Fong Fu ^b   Farmer, Marsha S ^a   Peterson, Linda R ^a   Schaffer, Jean E ^a   Ory, Daniel S ^a   Jiang, Xuntian ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b WASHINGTON UNIVERSITY   (United States)

Author keywords

Diabetes; Mass spectrometry; Sphingolipids; Validated assay; Very long chain ceramides

Indexed keywords

ELECTROSPRAY MASS SPECTROMETRY; HIGH-THROUGHPUT ASSAYS; LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC MS/MS); LONG CHAINS; LOWER LIMIT OF QUANTIFICATIONS; MULTIPLE REACTION MONITORING; PROTEIN PRECIPITATION; SPHINGOLIPIDS;

CALIBRATION; CHAINS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MEDICAL PROBLEMS; PLASMA (HUMAN); RNA;

CERAMIDES;

CERAMIDE; DEUTERIUM;

ADULT; ARTICLE; BLOOD; CALIBRATION; CASE CONTROL STUDY; CHEMISTRY; FEMALE; HUMAN; LIMIT OF DETECTION; LIQUID CHROMATOGRAPHY; LIQUID LIQUID EXTRACTION; MIDDLE AGED; NON INSULIN DEPENDENT DIABETES MELLITUS; OBESITY; PREMENOPAUSE; QUALITY CONTROL; REPRODUCIBILITY; STANDARD; TANDEM MASS SPECTROMETRY; VALIDATION STUDY;

ADULT; CALIBRATION; CASE-CONTROL STUDIES; CERAMIDES; CHROMATOGRAPHY, LIQUID; DEUTERIUM; DIABETES MELLITUS, TYPE 2; FEMALE; HUMANS; LIMIT OF DETECTION; LIQUID-LIQUID EXTRACTION; MIDDLE AGED; OBESITY; PREMENOPAUSE; QUALITY CONTROL; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; TANDEM MASS SPECTROMETRY;

EID: 84883551795     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-013-7166-9     Document Type: Article

References (42)

1. Kitatani K, Idkowiak-Baldys J, Hannun YA (2008) The sphingolipid salvage pathway in ceramide metabolism and signaling. Cell Signal 20:1010-1018
2. Grassme H, Riethmuller J, Gulbins E (2007) Biological aspects of ceramide-enriched membrane domains. Prog Lipid Res 46:161-170
3. Nikolova-Karakashian MN, Rozenova KA (2010) Ceramide in stress response. Adv Exp Med Biol 688:86-108
4. Arana L, Gangoiti P, Ouro A, Trueba M, Gomez-Munoz A (2010) Ceramide and ceramide 1-phosphate in health and disease. Lipids Health Dis 9:15
5. Ben-David O, Futerman AH (2010) The role of the ceramide acyl chain length in neurodegeneration: involvement of ceramide synthases. Neuromolecular Med 12:341-350
6. Krivit W, Hammarstrom S (1972) Identification and quantitation of free ceramides in human platelets. J Lipid Res 13:525-530
7. Quehenberger O, Armando AM, Brown AH, Milne SB, Myers DS, Merrill AH, Bandyopadhyay S, Jones KN, Kelly S, Shaner RL, Sullards CM, Wang E, Murphy RC, Barkley RM, Leiker TJ, Raetz CR, Guan Z, Laird GM, Six DA, Russell DW, McDonald JG, Subramaniam S, Fahy E, Dennis EA (2010) Lipidomics reveals a remarkable diversity of lipids in human plasma. J Lipid Res 51:3299-3305
8. Kolak M, Westerbacka J, Velagapudi VR, Wagsater D, Yetukuri L, Makkonen J, Rissanen A, Hakkinen AM, Lindell M, Bergholm R, Hamsten A, Eriksson P, Fisher RM, Oresic M, Yki-Jarvinen H (2007) Adipose tissue inflammation and increased ceramide content characterize subjects with high liver fat content independent of obesity. Diabetes 56:1960-1968
9. Haus JM, Kashyap SR, Kasumov T, Zhang R, Kelly KR, Defronzo RA, Kirwan JP (2009) Plasma ceramides are elevated in obese subjects with type 2 diabetes and correlate with the severity of insulin resistance. Diabetes 58:337-343
10. Holland WL, Brozinick JT, Wang LP, Hawkins ED, Sargent KM, Liu Y, Narra K, Hoehn KL, Knotts TA, Siesky A, Nelson DH, Karathanasis SK, Fontenot GK, Birnbaum MJ, Summers SA (2007) Inhibition of ceramide synthesis ameliorates glucocorticoid-, saturated-fat-, and obesity-induced insulin resistance. Cell Metab 5:167-179
11. Olivier S, Fillet M, Malaise M, Piette J, Bours V, Merville MP, Franchimont N (2005) Sodium nitroprusside-induced osteoblast apoptosis is mediated by long chain ceramide and is decreased by raloxifene. Biochem Pharmacol 69:891-901
12. Haynes CA, Allegood JC, Park H, Sullards MC (2009) Sphingolipidomics: methods for the comprehensive analysis of sphingolipids. J Chromatogr B Analyt Technol Biomed Life Sci 877:2696-2708
13. He X, Dagan A, Gatt S, Schuchman EH (2005) Simultaneous quantitative analysis of ceramide and sphingosine in mouse blood by naphthalene-2,3- dicarboxyaldehyde derivatization after hydrolysis with ceramidase. Anal Biochem 340:113-122
14. Kindt E, Wetterau J, Mueller SB, Castle C, Boustany-Kari CM (2010) Quantitative sphingosine measurement as a surrogate for total ceramide concentration-preclinical and potential translational applications. Biomed Chromatogr 24:752-758
15. Yoo HH, Son J, Kim DH (2006) Liquid chromatography-tandem mass spectrometric determination of ceramides and related lipid species in cellular extracts. J Chromatogr B Analyt Technol Biomed Life Sci 843:327-333
16. Merrill AH Jr, Sullards MC, Allegood JC, Kelly S, Wang E (2005) Sphingolipidomics: high-throughput, structure-specific, and quantitative analysis of sphingolipids by liquid chromatography tandem mass spectrometry. Methods 36:207-224
17. Farwanah H, Kolter T, Sandhoff K (2011) Mass spectrometric analysis of neutral sphingolipids: methods, applications, and limitations. Biochim Biophys Acta 1811:854-860
18. Tserng KY, Griffin R (2003) Quantitation and molecular species determination of diacylglycerols, phosphatidylcholines, ceramides, and sphingomyelins with gas chromatography. Anal Biochem 323:84-93
19. Vieu C, Terce F, Chevy F, Rolland C, Barbaras R, Chap H, Wolf C, Perret B, Collet X (2002) Coupled assay of sphingomyelin and ceramide molecular species by gas liquid chromatography. J Lipid Res 43:510-522
20. Yamane M (2003) Simultaneous quantitative determination method for ceramide species from crude cellular extracts by high-performance liquid chromatography-thermospray mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 783:181-190
21. Bielawski J, Szulc ZM, Hannun YA, Bielawska A (2006) Simultaneous quantitative analysis of bioactive sphingolipids by high-performance liquid chromatography-tandem mass spectrometry. Methods 39:82-91
22. Han X (2002) Characterization and direct quantitation of ceramide molecular species from lipid extracts of biological samples by electrospray ionization tandem mass spectrometry. Anal Biochem 302:199-212
23. Samuelsson K, Sameulsson B (1970) Gas chromatographic and mass spectrometric studies of synthetic and naturally occurring ceramides. Chem Phys Lipids 5:44-79
24. Bruce CR, Thrush AB, Mertz VA, Bezaire V, Chabowski A, Heigenhauser GJ, Dyck DJ (2006) Endurance training in obese humans improves glucose tolerance and mitochondrial fatty acid oxidation and alters muscle lipid content. Am J Physiol Endocrinol Metab 291:E99-E107
25. Cowart LA, Szulc Z, Bielawska A, Hannun YA (2002) Structural determinants of sphingolipid recognition by commercially available anti-ceramide antibodies. J Lipid Res 43:2042-2048
26. Summers SA (2006) Ceramides in insulin resistance and lipotoxicity. Prog Lipid Res 45:42-72
27. US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research and Center for Veterinary Medicine (2001) Guidance for Industry: Bioanalytical Method Validations. http://www.fda.gov/downloads/Drugs/Guidances/ucm070107.pdf. Accessed 6 May 2013
28. Lee JW, Devanarayan V, Barrett YC, Weiner R, Allinson J, Fountain S, Keller S, Weinryb I, Green M, Duan L, Rogers JA, Millham R, O'Brien PJ, Sailstad J, Khan M, Ray C, Wagner JA (2006) Fit-for-purpose method development and validation for successful biomarker measurement. Pharm Res 23:312-328
29. Lee JW, Weiner RS, Sailstad JM, Bowsher RR, Knuth DW, O'Brien PJ, Fourcroy JL, Dixit R, Pandite L, Pietrusko RG, Soares HD, Quarmby V, Vesterqvist OL, Potter DM, Witliff JL, Fritche HA, O'Leary T, Perlee L, Kadam S, Wagner JA (2005) Method validation and measurement of biomarkers in nonclinical and clinical samples in drug development: a conference report. Pharm Res 22:499-511
30. Kaufmann A, Butcher P, Maden K, Widmer M, Giles K, Uria D (2009) Are liquid chromatography/electrospray tandem quadrupole fragmentation ratios unequivocal confirmation criteria? Rapid Commun Mass Spectrom 23:985-998
31. Liebisch G, Drobnik W, Reil M, Trumbach B, Arnecke R, Olgemoller B, Roscher A, Schmitz G (1999) Quantitative measurement of different ceramide species from crude cellular extracts by electrospray ionization tandem mass spectrometry (ESI-MS/MS). J Lipid Res 40:1539-1546
32. Viswanathan CT, Bansal S, Booth B, DeStefano AJ, Rose MJ, Sailstad J, Shah VP, Skelly JP, Swann PG, Weiner R (2007) Quantitative bioanalytical methods validation and implementation: best practices for chromatographic and ligand binding assays. Pharm Res 24:1962-1973
33. Kushnir MM, Rockwood AL, Nelson GJ, Yue B, Urry FM (2005) Assessing analytical specificity in quantitative analysis using tandem mass spectrometry. Clin Biochem 38:319-327
34. Vogeser M, Seger C (2010) Pitfalls associated with the use of liquid chromatography-tandem mass spectrometry in the clinical laboratory. Clin Chem 56:1234-1244
35. Liu L, Zhang Y, Chen N, Shi X, Tsang B, Yu YH (2007) Upregulation of myocellular DGAT1 augments triglyceride synthesis in skeletal muscle and protects against fat-induced insulin resistance. J Clin Invest 117:1679-1689
36. Pickersgill L, Litherland GJ, Greenberg AS, Walker M, Yeaman SJ (2007) Key role for ceramides in mediating insulin resistance in human muscle cells. J Biol Chem 282:12583-12589
37. Powell DJ, Turban S, Gray A, Hajduch E, Hundal HS (2004) Intracellular ceramide synthesis and protein kinase Czeta activation play an essential role in palmitate-induced insulin resistance in rat L6 skeletal muscle cells. Biochem J 382:619-629
38. Ussher JR, Koves TR, Cadete VJ, Zhang L, Jaswal JS, Swyrd SJ, Lopaschuk DG, Proctor SD, Keung W, Muoio DM, Lopaschuk GD (2010) Inhibition of de novo ceramide synthesis reverses diet-induced insulin resistance and enhances whole-body oxygen consumption. Diabetes 59:2453-2464
39. Dube JJ, Amati F, Stefanovic-Racic M, Toledo FG, Sauers SE, Goodpaster BH (2008) Exercise-induced alterations in intramyocellular lipids and insulin resistance: the athlete's paradox revisited. Am J Physiol Endocrinol Metab 294:E882-888
40. Gorska M, Dobrzyn A, Zendzian-Piotrowska M, Namiot Z (2002) Concentration and composition of free ceramides in human plasma. Horm Metab Res 34:466-468
41. Hartmann D, Lucks J, Fuchs S, Schiffmann S, Schreiber Y, Ferreiros N, Merkens J, Marschalek R, Geisslinger G, Grosch S (2012) Long chain ceramides and very long chain ceramides have opposite effects on human breast and colon cancer cell growth. Int J Biochem Cell Biol 44:620-628
42. Mielke MM, Haughey NJ, Ratnam Bandaru VV, Schech S, Carrick R, Carlson MC, Mori S, Miller MI, Ceritoglu C, Brown T, Albert M, Lyketsos CG (2010) Plasma ceramides are altered in mild cognitive impairment and predict cognitive decline and hippocampal volume loss. Alzheimers Dement 6:378-385