PTHrP in differentiating human mesenchymal stem cells: Transcript isoform expression, promoter methylation, and protein accumulation

Biochimie

Volumn 95, Issue 10, 2013, Pages 1888-1896

  Longo, Alessandra ^a   Librizzi, Mariangela ^a   Naselli, Flores ^a   Caradonna, Fabio ^a   Tobiasch, Edda ^b   Luparello, Claudio ^a  

^a UNIVERSITY OF PALERMO   (Italy)

^b BONN RHEIN SIEG UNIVERSITY OF APPLIED SCIENCES   (Germany)

Author keywords

Adipogenesis; Gene expression; Mesenchymal stem cells; Osteogenesis; Promoter methylation; PTHrP

Indexed keywords

GENOMIC DNA; PROTEIN;

ADIPOGENESIS; ARTICLE; BONE CELL; BONE DEVELOPMENT; CELL DIFFERENTIATION; EXON; GENE; GENE EXPRESSION; HUMAN; HUMAN CELL; MESENCHYMAL STEM CELL; METHYLATION; PROMOTER REGION; PTHRP GENE;

3,3′,5,5′-TETRAMETHYLBENZIDINE; 5-BROMO-4-CHLORO-3′-INDOLYPHOSPHATE; ADIPOGENESIS; BCIP; BMP; BONE MORPHOGENETIC PROTEIN; D-PBS; DEOXYNUCLEOTIDE TRIPHOSPHATES; DNTPS; DULBECCO'S PHOSPHATE BUFFERED SALINE; EDTA; ETHYLENEDIAMINETETRAACETIC ACID; FCS; FETAL CALF SERUM; GENE EXPRESSION; MESENCHYMAL STEM CELLS; METHYLATION-SENSITIVE RESTRICTION ENDONUCLEASE; MICE; MICROTITER IMMUNOCYTOCHEMICAL ELISA ASSAY; MSC; MSRE; NBT; NITRO-BLUE TETRAZOLIUM; OSTEOGENESIS; PARATHYROID HORMONE-RELATED PROTEIN; PCR; PHENYLMETHYLSULFONYL FLUORIDE; PMSF; POLYMERASE CHAIN REACTION; PROMOTER METHYLATION; PTHRP; S.E.M.; SDS; SEMI-QUANTITATIVE MULTIPLEX; SM; SODIUM DODECYL SULFATE; STANDARD ERROR OF THE MEAN; TBS-T; TMB; TRIS-BUFFERED SALINE-TWEEN 20;

ADIPOCYTES; ALTERNATIVE SPLICING; CELL DIFFERENTIATION; CELLS, CULTURED; CPG ISLANDS; DNA METHYLATION; EXONS; GENE EXPRESSION REGULATION; HUMANS; INTRONS; MESENCHYMAL STROMAL CELLS; OSTEOCYTES; PARATHYROID HORMONE-RELATED PROTEIN; PROMOTER REGIONS, GENETIC; PROTEIN ISOFORMS; TRANSCRIPTION, GENETIC;

EID: 84883250902     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2013.06.014     Document Type: Article

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