The PEG-switch assay: A fast semi-quantitative method to determine protein reversible cysteine oxidation

Journal of Pharmacological and Toxicological Methods

Volumn 68, Issue 3, 2013, Pages 297-301

  Burgoyne, Joseph Robert ^a   Oviosu, Olujimi ^a   Eaton, Philip ^a  

^a KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

Kinases; Oxidation; PEG switch; Thiols; Western blotting

Indexed keywords

CALSEQUESTRIN; CYCLIC GMP DEPENDENT PROTEIN KINASE 1; CYSTEINE; HYDROGEN PEROXIDE; MACROGOL MALEIMIDE; MALEIMIDE DERIVATIVE; PROTEIN KINASE; PROTEIN PKARI; THIOL DERIVATIVE; UNCLASSIFIED DRUG;

ALKYLATION; ANIMAL TISSUE; ARTICLE; ASSAY; CHEMICAL LABELING; CONTROLLED STUDY; EMBRYO; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; NONHUMAN; OXIDATION; OXIDATION REDUCTION REACTION; PEG SWITCH ASSAY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DETERMINATION; PROTEIN PROCESSING; PROTEIN TARGETING; QUANTITATIVE ANALYSIS; RAT; WESTERN BLOTTING;

KINASES; OXIDATION; PEG-SWITCH; THIOLS; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CELLS, CULTURED; CYCLIC AMP; CYCLIC GMP; CYSTEINE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; HYDROGEN PEROXIDE; MALEIMIDES; OXIDATION-REDUCTION; POLYETHYLENE GLYCOLS; PROTEIN KINASES; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEINS; RATS; SULFHYDRYL COMPOUNDS;

EID: 84883244717     PISSN: 10568719     EISSN: 1873488X     Source Type: Journal    
DOI: 10.1016/j.vascn.2013.07.001     Document Type: Article

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