Measurement of steroids in rats after exposure to an endocrine disruptor: Mass spectrometry and radioimmunoassay demonstrate similar results

Journal of Pharmacological and Toxicological Methods

Volumn 68, Issue 3, 2013, Pages 314-322

  Riffle, Brandy W ^a,c   Henderson, W Matthew ^b   Laws, Susan C ^c  

^a OAK RIDGE INSTITUTE FOR SCIENCE AND EDUCATION   (United States)

^b U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

^c U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

Author keywords

Mass spectroscopy; Methods; Quality control; Radioimmunoassay; Steroids

Indexed keywords

ANDROSTENEDIONE; ATRAZINE; CORTICOSTERONE; DANSYL CHLORIDE; ENDOCRINE DISRUPTOR; ESTRADIOL; ESTRONE; METHYLCELLULOSE; PROGESTERONE; TESTOSTERONE;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; CORRELATION COEFFICIENT; ELECTROSPRAY MASS SPECTROMETRY; EXPOSURE; FEMALE; INTERMETHOD COMPARISON; LIQUID CHROMATOGRAPHY; MALE; NONHUMAN; RADIOIMMUNOASSAY; RAT; STEROID ANALYSIS; STEROID BLOOD LEVEL; STEROID URINE LEVEL; TOXICITY TESTING;

ANALYSIS OF VARIANCE; ANDRO; ANDROSTENEDIONE; ANOVA; AREA UNDER THE CURVE; ATR; ATRAZINE; AUC; COEFFICIENT OF VARIATION; CORT; CORTICOSTERONE; CV; E1; E2; EDC; ENDOCRINE DISRUPTING CHEMICAL; ESTRADIOL; ESTRONE; FEDERAL INSECTICIDE, FUNGICIDE, AND RODENTICIDE ACT SCIENTIFIC ADVISORY PANEL; FIFRA SAP; GD; GENERAL LINEAR MODEL; GESTATIONAL DAY; GLM; HPA; HYPOTHALAMIC-PITUITARY-ADRENAL; ICC; INTRACLASS CORRELATION COEFFICIENTS; LC-MS/MS; LH; LIQUID CHROMATOGRAPHY COUPLED WITH TANDEM MASS SPECTROSCOPY; LUTEINIZING HORMONE; MASS SPECTROSCOPY; METHODS; MRM; MULTIPLE REACTION MONITORING MODE; P4; PROGESTERONE; QUALITY CONTROL; RADIOIMMUNOASSAY; RIA; SD; SELECTIVE REACTION MONITORING; SEM; SOLID PHASE EXTRACTION; SPE; SRM; STANDARD DEVIATION; STANDARD ERROR MEAN; STEROIDS; T; TESTOSTERONE;

ADMINISTRATION, ORAL; ANIMALS; ATRAZINE; CHROMATOGRAPHY, LIQUID; CORTICOSTERONE; ENDOCRINE DISRUPTORS; MALE; RADIOIMMUNOASSAY; RATS; RATS, WISTAR; REPRODUCIBILITY OF RESULTS; STEROIDS; TANDEM MASS SPECTROMETRY;

EID: 84883225206     PISSN: 10568719     EISSN: 1873488X     Source Type: Journal    
DOI: 10.1016/j.vascn.2013.07.003     Document Type: Article

References (50)

1. Bland J.M., Altman D.G. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1986, 1:307-310.
2. Boeniger M.F., Lowry L.K., Rosenberg J. Interpretation of urine results used to assess chemical exposure with emphasis on creatinine adjustments: A review. American Industrial Hygiene Association Journal 1993, 54:615-627.
3. Chang Y.C., Li C.M., Li L.A., Jong S.B., Liao P.C., Chang L.W. Quantitative measurement of male steroid hormones using automated on-line solid phase extraction-liquid chromatography-tandem mass spectrometry and comparison with radioimmunoassay. The Analyst 2003, 128:363-368.
4. Cooper R. A proposed MOA for atrazine and atrazine metabolites 2010, Vol. 2013. USEPA.
5. Cooper R.L., Laws S.C., Das P.C., Narotsky M.G., Goldman J.M., Lee Tyrey E., et al. Atrazine and reproductive function: Mode and mechanism of action studies. Birth Defects Research. Part B, Developmental and Reproductive Toxicology 2007, 80:98-112.
6. Cooper R.L., Stoker T.E., Goldman J.M., Parrish M.B., Tyrey L. Effect of atrazine on ovarian function in the rat. Reproductive Toxicology 1996, 10:257-264.
7. Cooper R.L., Stoker T.E., Tyrey L., Goldman J.M., McElroy W.K. Atrazine disrupts the hypothalamic control of pituitary-ovarian function. Toxicological Sciences 2000, 53:297-307.
8. De Coster S., van Larebeke N. Endocrine-disrupting chemicals: Associated disorders and mechanisms of action. Journal of Environmental and Public Health 2012, 2012:713696.
9. Dorgan J.F., Fears T.R., McMahon R.P., Aronson Friedman L., Patterson B.H., Greenhut S.F. Measurement of steroid sex hormones in serum: A comparison of radioimmunoassay and mass spectrometry. Steroids 2002, 67:151-158.
10. Eldridge J.C., Tennant M.K., Wetzel L.T., Breckenridge C.B., Stevens J.T. Factors affecting mammary tumor incidence in chlorotriazine-treated female rats: Hormonal properties, dosage, and animal strain. Environmental Health Perspectives 1994, 102(Suppl. 11):29-36.
11. Etter M.L., Eichhorst J., Lehotay D.C. Clinical determination of 17-hydroxyprogesterone in serum by LC-MS/MS: Comparison to Coat-A-Count RIA method. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 2006, 840:69-74.
12. Falk R.T., Gail M.H., Fears T.R., Rossi S.C., Stanczyk F., Adlercreutz H., et al. Reproducibility and validity of radioimmunoassays for urinary hormones and metabolites in pre- and postmenopausal women. Cancer Epidemiology, Biomarkers & Prevention 1999, 8:567-577.
13. Faupel-Badger J.M., Fuhrman B.J., Xu X., Falk R.T., Keefer L.K., Veenstra T.D., et al. Comparison of liquid chromatography-tandem mass spectrometry, RIA, and ELISA methods for measurement of urinary estrogens. Cancer Epidemiology, Biomarkers & Prevention 2010, 19:292-300.
14. Flores O.R., Sun L., Vaziri N.D., Miyada D.S. Colorimetric rate method for the determination of creatinine as implemented by the Beckman Creatinine Analyzer 2. The American Journal of Medical Technology 1980, 46:792-798.
15. Fraites M.J., Cooper R.L., Buckalew A., Jayaraman S., Mills L., Laws S.C. Characterization of the hypothalamic-pituitary-adrenal axis response to atrazine and metabolites in the female rat. Toxicological Sciences 2009, 112:88-99.
16. Guo T., Gu J., Soldin O.P., Singh R.J., Soldin S.J. Rapid measurement of estrogens and their metabolites in human serum by liquid chromatography-tandem mass spectrometry without derivatization. Clinical Biochemistry 2008, 41:736-741.
17. Gust M., Vulliet E., Giroud B., Garnier F., Couturier S., Garric J., et al. Development, validation and comparison of LC-MS/MS and RIA methods for quantification of vertebrates-like sex-steroids in prosobranch molluscs. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 2010, 878:1487-1492.
18. Haisenleder D.J., Schoenfelder A.H., Marcinko E.S., Geddis L.M., Marshall J.C. Estimation of estradiol in mouse serum samples: Evaluation of commercial estradiol immunoassays. Endocrinology 2011, 152:4443-4447.
19. Handa R.J. Effects of atrazine on neuroendocrine function in male and female rats 2010, Vol. 2013. USEPA.
20. Hopkins W. Calculating the reliability intraclass correlation coefficient and its confidence limits (Excel spreadsheet) 2009.
21. Hotchkiss M.G., Best D.S., Cooper R.L., Laws S.C. Atrazine does not induce pica behavior at doses that increase hypothalamic-pituitary-adrenal axis activation and cause conditioned taste avoidance. Neurotoxicology and Teratology 2012, 34:295-302.
22. Hsing A.W., Stanczyk F.Z., Belanger A., Schroeder P., Chang L., Falk R.T., et al. Reproducibility of serum sex steroid assays in men by RIA and mass spectrometry. Cancer Epidemiology, Biomarkers & Prevention 2007, 16:1004-1008.
23. Janse F., Eijkemans M.J., Goverde A.J., Lentjes E.G., Hoek A., Lambalk C.B., et al. Assessment of androgen concentration in women: Liquid chromatography-tandem mass spectrometry and extraction RIA show comparable results. European Journal of Endocrinology 2011, 165:925-933.
24. Khosla S., Amin S., Singh R.J., Atkinson E.J., Melton L.J., Riggs B.L. Comparison of sex steroid measurements in men by immunoassay versus mass spectroscopy and relationships with cortical and trabecular volumetric bone mineral density. Osteoporosis International 2008, 19:1465-1471.
25. Kushnir M.M., Rockwood A.L., Bergquist J. Liquid chromatography-tandem mass spectrometry applications in endocrinology. Mass Spectrometry Reviews 2010, 29:480-502.
26. Laws S.C., Ferrell J.M., Stoker T.E., Cooper R.L. Pubertal development in female Wistar rats following exposure to propazine and atrazine biotransformation by-products, diamino-S-chlorotriazine and hydroxyatrazine. Toxicological Sciences 2003, 76:190-200.
27. Laws S.C., Ferrell J.M., Stoker T.E., Schmid J., Cooper R.L. The effects of atrazine on female wistar rats: An evaluation of the protocol for assessing pubertal development and thyroid function. Toxicological Sciences 2000, 58:366-376.
28. Laws S.C., Hotchkiss M., Ferrell J., Jayaraman S., Mills L., Modic W., et al. Chlorotriazine herbicides and metabolites activate an ACTH-dependent release of corticosterone in male Wistar rats. Toxicological Sciences 2009, 112:78-87.
29. Miller J.J., Valdes R. Approaches to minimizing interference by cross-reacting molecules in immunoassays. Clinical Chemistry 1991, 37:144-153.
30. Modic W. The role of testicular aromatase in the atrazine mediated changes of estrone and estradiol in the male Wistar rat 2004, North Carolina State University.
31. Pruett S., Hebert P., Lapointe J.M., Reagan W., Lawton M., Kawabata T.T. Characterization of the action of drug-induced stress responses on the immune system: Evaluation of biomarkers for drug-induced stress in rats. Journal of Immunotoxicology 2007, 4:25-38.
32. Pruett S., Lapointe J.M., Reagan W., Lawton M., Kawabata T.T. Urinary corticosterone as an indicator of stress-mediated immunological changes in rats. Journal of Immunotoxicology 2008, 5:17-22.
33. Rauh M., Groschl M., Rascher W., Dorr H.G. Automated, fast and sensitive quantification of 17 alpha-hydroxy-progesterone, androstenedione and testosterone by tandem mass spectrometry with on-line extraction. Steroids 2006, 71:450-458.
34. Rosner B. Fundamentals of biostatistics 2005, Duxbury Press, Belmont, CA.
35. Rosner W., Auchus R.J., Azziz R., Sluss P.M., Raff H. Position statement: Utility, limitations, and pitfalls in measuring testosterone: An endocrine society position statement. The Journal of Clinical Endocrinology and Metabolism 2007, 92:405-413.
36. Sampat M.P., Whitman G.J., Stephens T.W., Broemeling L.D., Heger N.A., Bovik A.C., et al. The reliability of measuring physical characteristics of spiculated masses on mammography. The British Journal of Radiology 2006, 79(Spec No 2):S134-140.
37. Santa Maria C., Vilas M.G., Muriana F.G., Relimpio A. Subacute atrazine treatment effects on rat renal functions. Bulletin of Environmental Contamination and Toxicology 1986, 36:325-331.
38. Santen R.J., Demers L., Ohorodnik S., Settlage J., Langecker P., Blanchett D., et al. Superiority of gas chromatography/tandem mass spectrometry assay (GC/MS/MS) for estradiol for monitoring of aromatase inhibitor therapy. Steroids 2007, 72:666-671.
39. Shackleton C. Clinical steroid mass spectrometry: A 45-year history culminating in HPLC-MS/MS becoming an essential tool for patient diagnosis. The Journal of Steroid Biochemistry and Molecular Biology 2010, 121:481-490.
40. Singh R.J. Validation of a high throughput method for serum/plasma testosterone using liquid chromatography tandem mass spectrometry (LC-MS/MS). Steroids 2008, 73:1339-1344.
41. Soldin S.J., Soldin O.P. Steroid hormone analysis by tandem mass spectrometry. Clinical Chemistry 2009, 55:1061-1066.
42. Stanczyk F.Z., Lee J.S., Santen R.J. Standardization of steroid hormone assays: Why, how, and when?. Cancer Epidemiology, Biomarkers & Prevention 2007, 16:1713-1719.
43. Stenman U.H. Immunoassay standardization: Is it possible, who is responsible, who is capable?. Clinical Chemistry 2001, 47:815-820.
44. Stoker T.E., Guidici D.L., Laws S.C., Cooper R.L. The effects of atrazine metabolites on puberty and thyroid function in the male Wistar rat. Toxicological Sciences 2002, 67:198-206.
45. Stoker T.E., Laws S.C., Guidici D.L., Cooper R.L. The effect of atrazine on puberty in male Wistar rats: An evaluation in the protocol for the assessment of pubertal development and thyroid function. Toxicological Sciences 2000, 58:50-59.
46. Taylor R.L., Machacek D., Singh R.J. Validation of a high-throughput liquid chromatography-tandem mass spectrometry method for urinary cortisol and cortisone. Clinical Chemistry 2002, 48:1511-1519.
47. USEPA Re- Evaluation of Human Health Effects of Atrazine: Review of Experimental Animal and In Vitro Studies and Drinking Water Monitoring Frequency 2010, 486. F. Federal Insecticide, and Rodenticide Act Science Advisory Panel (FIFRA SAP) (Ed.).
48. Weir J.P. Quantifying test-retest reliability using the intraclass correlation coefficient and the SEM. Journal of Strength and Conditioning Research 2005, 19:231-240.
49. Wetzel L.T., Luempert L.G., Breckenridge C.B., Tisdel M.O., Stevens J.T., Thakur A.K., et al. Chronic effects of atrazine on estrus and mammary tumor formation in female Sprague-Dawley and Fischer 344 rats. Journal of Toxicology and Environmental Health 1994, 43:169-182.
50. WHO Global assessment of the state-of-the-science of endocrine disruptors 2002, Vol. 2013. World Health Organization.