Enzyme-gold nanoparticle bioconjugates: Quantification of particle stoichiometry and enzyme specific activity

Methods in Molecular Biology

Volumn 1026, Issue , 2013, Pages 163-174

  Keighron, Jacqueline D ^a   Keating, Christine D ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

Bioconjugate; Enzyme; Gold; Nanoparticle; Specific activity

Indexed keywords

5,5' DITHIOBIS(2 NITROBENZOIC ACID); ACETYL COENZYME A; BICARBONATE; CITRATE SYNTHASE; ENZYME; GOLD NANOPARTICLE; OXALOACETIC ACID; POTASSIUM CYANIDE; TROMETAMOL;

ARTICLE; BIOCATALYST; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME GOLF NANOPARTICLE BIOCONJUGATE; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME SUBSTRATE; ENZYME SYNTHESIS; FLOCCULATION; IONIC STRENGTH; MOLECULAR WEIGHT; PRIORITY JOURNAL; SPECTROPHOTOMETER; STOICHIOMETRY;

EID: 84883183845     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-468-5_13     Document Type: Article

References (11)

1. Willner I, Bazaar B, Willner B (2007) Nanoparticle-enzyme hybrid systems for nanobiotechnology. FEBS J 274:302-309
2. Keighron JD, Keating CD (2010) Enzyme: Nanoparticle bioconjugates with two sequential enzymes: Stoichiometry and activity of malate dehydrogenase and citrate synthase on Au nanoparticles. Langmuir 26:18992-19000
3. Vertegal AA, Siegel RW, Dordick JS (2004) Silica nanoparticle size in fluences on the structure and enzymatic activity of adsorbed lysozyme. Langmuir 20:6800-6807
4. Cans A-S, Dean SL, Reyes FE, Keating CD (2007) Synthesis and characterization of enzyme-Au bioconjugates: HRP an fluoresceinlabeled HP. NanoBiotechnology 3:12-22
5. Hayat MA (1989) Colloidal gold principles, methods, and applications, vol 1-3. Academic, San Diego, CA
6. Keating CD, Musick MD, Keefe MH, Natan MJ (1999) Kinetic and thermodynamics of Au colloid monolayer self-assembly: Undergraduate experiments in surface and nanomaterials chemistry. J Chem Educ 76: 949-955
7. Hermanson GT (1996) Bioconjugate techniques. Academic, San Diego, CA
8. Santoro N, Brtva T, Vander Roest S, Siegel K, Waldrop GL (2006) A high-throughput screening assay for the carboxyltransferase subunit of acetyl-CoA carboxylase. Anal Chem 354:70-77
9. Ted Pella Inc. www.tedpella.com . Accessed May 2010
10. De Roe C, Courtoy PJ, Baudhuin P (1987) A model of protein-colloidal gold interactions. J Histochem Cytochem 35:1191-1198
11. Demers LM, Mirkin CA, Mucic RC, Reynolds RA III, Letsinger RL, Elghanian R, Viswanadham G (2000) A fluorescence-based method for determining the surface coverage and hybridization ef ficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles. Anal Chem 72:5535-5541