Revealing neuronal circuitry using stem cell-derived neurons

Current Protocols in Stem Cell Biology

Volumn 1, Issue SUPPL.25, 2013, Pages

  Garcia, Isabella ^a   Kim, Cynthia ^a   Arenkiel, Benjamin R ^a,b  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b TEXAS CHILDREN S HOSPITAL   (United States)

Author keywords

Circuit; Embryonic stem cells; Monosynaptic; Neuron; Rabies; Rabies G; Retrograde; Synapse; TVA

Indexed keywords

BALANCED SALT SOLUTION; GELATIN; GLASS; POLY DEXTRO LYSINE; POLYLYSINE;

ANIMAL CELL; ARTICLE; BRAIN CELL; BRAIN SLICE; BRAIN TISSUE; CELL SUSPENSION; DNA MODIFICATION; ELECTROPORATION; EMBRYONIC STEM CELL; EMBRYONIC STEM CELL DERIVED NEURON; EX VIVO STUDY; FEEDER CELL; FIBROBLAST; GLIA CELL; IN VITRO STUDY; IN VIVO STUDY; MOUSE; NERVE CELL CULTURE; NERVE CELL DIFFERENTIATION; NERVE CELL NETWORK; NEURAL STEM CELL; NONHUMAN; PETRI DISH; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; RABIES VIRUS; STABLE TRANSFECTION; SUSPENSION CELL CULTURE; SYNAPSE; SYNAPTOGENESIS; TISSUE CULTURE; WILD TYPE; ANIMAL; BRAIN; C57BL MOUSE; CELL CULTURE; CELL DIFFERENTIATION; CELL PROLIFERATION; CYTOLOGY; DRUG EFFECT; HUMAN; METABOLISM; NERVE CELL; TISSUE CULTURE TECHNIQUE;

RABIES VIRUS;

ANIMALS; BRAIN; CELL DIFFERENTIATION; CELL PROLIFERATION; CELLS, CULTURED; EMBRYONIC STEM CELLS; FEEDER CELLS; GELATIN; HUMANS; MICE; MICE, INBRED C57BL; NERVE NET; NEURONS; POLYLYSINE; SYNAPSES; TISSUE CULTURE TECHNIQUES;

EID: 84883173338     PISSN: 19417322     EISSN: 19388969     Source Type: Journal    
DOI: 10.1002/9780470151808.sc02d15s25     Document Type: Article

References (12)

1. Bissonnette, C.J., Lyass, L., Bhattacharyya, B.J., Belmadani, A., Miller, R.J., and Kessler, J.A. 2011. The controlled generation of functional basal forebrain cholinergic neurons from human embryonic stem cells. Stem Cells 29:802-811.
2. Caiazzo, M., Dell'Anno, M.T., Dvoretskova, E., Lazarevic, D., Taverna, S., Leo, D., Sotnikova, T.D., Menegon, A., Roncaglia, P., Colciago, G., Russo, G., Carninci, P., Pezzoli, G., Gainetdinov, R.R., Gustincich, S., Dityatev, A., and Broccoli, V. 2011. Direct generation of functional dopaminergic neurons from mouse and human fibroblasts. Nature 476:224-227.
3. Callaway, E.M. 2008. Transneuronal circuit tracing with neurotropic viruses. Curr. Opin. Neurobiol. 18:617-623.
4. Cho, E.G., Zaremba, J.D., McKercher, S.R., Talantova, M., Tu, S., Masliah, E., Chan, S.F., Nakanishi, N., Terskikh, A., and Lipton, S.A. 2011. MEF2C enhances dopaminergic neuron differentiation of human embryonic stem cells in a parkinsonian rat model. PLoS One 6: e24027.
5. Donovan, J. and Brown, P. 2006. Euthanasia. Curr. Protoc. Immunol. 73:1.8.1-1.8.4.
6. Garcia, I., Huang, L., Ung, K., and Arenkiel, B.R. 2012. Tracing synaptic connectivity onto embryonic stem cell-derived neurons. Stem Cells 30:2140-2151.
7. Phelan, M.C. 2006. Techniques for mammalian cell tissue culture. Curr. Protoc. Mol. Biol. 74:A.3F.1-A.3F.18.
8. Takahashi, K. and Yamanaka, S. 2006. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663-676.
9. Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S. 2007. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861-872.
10. Tonnesen, J., Parish, C.L., Sorensen, A.T., Andersson, A., Lundberg, C., Deisseroth, K., Arenas, E., Lindvall, O., and Kokaia, M. 2011. Functional integration of grafted neural stem cellderived dopaminergic neurons monitored by optogenetics in an in vitro Parkinson model. PLoS One 6:e17560.
11. Wickersham, I.R., Finke, S., Conzelmann, K.K., and Callaway, E.M. 2007. Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat. Methods 4:47-49.
12. Wickersham, I.R., Lyon, D.C., Barnard, R.J., Mori, T., Finke, S., Conzelmann, K.K., Young, J.A., and Callaway, E.M. 2007. Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons. Neuron 53:639-647.