Extraction and processing of circulating DNA from large sample volumes using methylation on beads for the detection of rare epigenetic events

Clinica Chimica Acta

Volumn 425, Issue , 2013, Pages 169-175

  Keeley, Brian ^a   Stark, Alejandro ^b   Pisanic, Thomas R ^a   Kwak, Ruby ^c   Zhang, Yi ^b,d   Wrangle, John ^c   Baylin, Stephen ^c   Herman, James ^c   Ahuja, Nita ^c   Brock, Malcolm V ^c   Wang, Tza Huei ^a,b  

^a Johns Hopkins University   (United States)

^b Johns Hopkins University   (United States)

^c JOHNS HOPKINS UNIVERSITY   (United States)

^d INSTITUTE OF BIOENGINEERING AND NANOTECHNOLOGY   (Singapore)

Author keywords

Blood processing protocols; Cancer epigenetics; Circulating DNA; DNA methylation; Methylation specific PCR; Quantification

Indexed keywords

ALCOHOL; APC PROTEIN; CHLOROFORM; DNA; GENOMIC DNA; PHENOL; RAS ASSOCIATION DOMAIN FAMILY PROTEIN 1A;

ADENOMATOUS POLYPOSIS COLI GENE; ADULT; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; DNA EXTRACTION; DNA METHYLATION; FEMALE; HUMAN; INTERMETHOD COMPARISON; LUNG NON SMALL CELL CANCER; MALE; METHYLATION ON BEADS EXTRACTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RASSF1A GENE; SENSITIVITY AND SPECIFICITY;

BLOOD-PROCESSING PROTOCOLS; CANCER EPIGENETICS; CIRCULATING DNA; DNA METHYLATION; METHYLATION SPECIFIC PCR; QUANTIFICATION;

ACTINS; AGED; CARCINOMA, NON-SMALL-CELL LUNG; DNA; DNA METHYLATION; EPIGENESIS, GENETIC; FEMALE; GENE EXPRESSION; HELA CELLS; HUMANS; LUNG NEOPLASMS; MALE; MICROSPHERES; MIDDLE AGED; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SULFITES; TUMOR MARKERS, BIOLOGICAL; TUMOR SUPPRESSOR PROTEINS;

EID: 84883029814     PISSN: 00098981     EISSN: 18733492     Source Type: Journal    
DOI: 10.1016/j.cca.2013.07.023     Document Type: Article

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