Human defined antigenic region on the nucleoprotein of Crimean-Congo hemorrhagic fever virus identified using truncated proteins and a bioinformatics approach

Journal of Virological Methods

Volumn 193, Issue 2, 2013, Pages 706-712

  Burt, F J ^a,b   Samudzi, R R ^a   Randall, C ^a   Pieters, D ^a   Vermeulen, J ^a   Knox, C M ^c  

^a UNIVERSITY OF THE FREE STATE   (South Africa)

^b NATIONAL HEALTH LABORATORY SERVICE   (South Africa)

^c RHODES UNIVERSITY   (South Africa)

Author keywords

Antigenicity; Crimean Congo hemorrhagic fever virus; Nucleoprotein

Indexed keywords

EPITOPE; IMMUNOGLOBULIN G ANTIBODY; RECOMBINANT ANTIGEN; VIRUS NUCLEOPROTEIN;

ANTIBODY COMBINING SITE; ANTIBODY DETECTION; ANTIBODY RESPONSE; ARTICLE; BIOINFORMATICS; CARBOXY TERMINAL SEQUENCE; CLINICAL ARTICLE; CONTROLLED STUDY; CRIMEAN CONGO HEMORRHAGIC FEVER; ENZYME LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; HUMAN; HYDROPHOBICITY; NONHUMAN; PREDICTION; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS; VIRUS IDENTIFICATION;

ANTIGENICITY; CRIMEAN-CONGO HEMORRHAGIC FEVER VIRUS; NUCLEOPROTEIN;

ANTIBODIES, VIRAL; ANTIGENS, VIRAL; COMPUTATIONAL BIOLOGY; DNA MUTATIONAL ANALYSIS; EPITOPE MAPPING; EPITOPES; HEMORRHAGIC FEVER VIRUS, CRIMEAN-CONGO; HUMANS; IMMUNOGLOBULIN G; NUCLEOPROTEINS; RECOMBINANT PROTEINS;

EID: 84882980672     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2013.07.055     Document Type: Article

References (16)

1. Amexis G., Young N.S. Multiple antigenic peptides as vaccine platform for the induction of humoral responses against dengue 2 virus. Viral Immunology 2007, 20:657-663.
2. Burt F.J., Leman P.A., Abbott J.C., Swanepoel R. Serodiagnosis of Crimean-Congo haemorrhagic fever. Epidemiology and Infection 1994, 113:551-562.
3. Burt F.J., Paweska J.T., Ashkettle B., Swanepoel R. Genetic relationship in southern African Crimean-Congo hemorrhagic fever virus isolates: evidence for occurrence of reassortment. Epidemiology and Infection 2009, 137:1302-1308.
4. Casey J.L., Coley A.M., Parisi K., Foley M. Peptide mimics selected from immune sera using phage display technology can replace native antigens in the diagnosis of Epstein-Barr virus infections. Protein Engineering, Design and Selection 2009, 22:85-91.
5. Clerx J.P., Casals J., Bishop D.H. Structural characteristics of nairoviruses (genus Nairovirus, Bunyaviridae). Journal of General Virology 1981, 55:165-178.
6. Guo H., Zhou E.M., Sun Z.F., Meng X.J. Immunodominant epitopes mapped by synthetic peptides on the capsid protein of avian hepatitis E virus are non-protective. Viral Immunology 2008, 21:61-67.
7. Honda E.R., Zanchi F., Rios K., Lira E., Deusilenevieira da Silva L.H., De Paula S.O. Design and heterologous expression of dengue virus envelope protein (E) peptides and their use for serological diagnosis. Journal of Virological Methods 2012, 186:55-61.
8. Hoogstraal H. The epidemiology of tick-borne Crimean-Congo hemorrhagic fever in Asia, Europe, and Africa. Journal of Medical Entomology 1979, 15:307-417.
9. Larsen J.E.P., Lund O., Nielsen M. Improved method for predicting linear B-cell epitopes. Immunome Research 2006, 24:2.
10. Maltezou H.C., Andonova L., Andraghetti R. Crimean-Congo hemorrhagic fever in Europe: current situation calls for preparedness. Eurosurveillance 2010, 15:19504-19508.
11. Pellequer J.L., Westhof E., Regenhof M.H. Predicting location of continuous epitopes in proteins from their primary structures. Methods in Enzymology 1991, 203:176-201.
12. Rangel da Silva A.N., Nascimento E.J., Cordeiro M.T., Gil L.H., Abath F.G., Montenegro S.M., Marques E.T. Identification of continuous human B-cell epitopes in the envelope glycoprotein of dengue virus type 3 (DENV-3). PLoS ONE 2009, 13:7425.
13. Samudzi R.R., Leman P.A., Paweska J.T., Swanepoel R., Burt F.J. Bacterial expression of Crimean-Congo hemorrhagic fever virus nucleoprotein and its evaluation as a diagnostic reagent in an indirect ELISA. Journal of Virological Methods 2012, 179:70-76.
14. Sanchez A.J., Vincent M.J., Nichol S.T. Characterization of the glycoproteins of Crimean-Congo hemorrhagic fever virus. Journal of Virology 2002, 76:7263-7275.
15. Singh A.K., Rath S.K., Misra K. Identification of epitopes in Indian human papilloma virus 16 E6: a bioinformatics approach. Journal of Virological Methods 2011, 177:26-30.
16. Wu H.-C., Jung M.-Y., Chiu C.-Y., Chao T.-T., Lai S.-C., Jan J.-T., Shaio M.-F. Identification of a dengue type 2 (DEN-2) serotype-specific B cell epitope and detection of DEN-2-immunized animal serum samples using an epitope based peptide antigen. Journal of General Virology 2003, 84:2771-2779.