Bacterial expression of Crimean-Congo hemorrhagic fever virus nucleoprotein and its evaluation as a diagnostic reagent in an indirect ELISA

Journal of Virological Methods

Volumn 179, Issue 1, 2012, Pages 70-76

  Samudzi, Rudo R ^a   Leman, Patricia A ^b   Paweska, Janusz T ^b   Swanepoel, Robert ^b   Burt, Felicity J ^a  

^a NATIONAL HEALTH LABORATORY SERVICE   (South Africa)

^b NATIONAL INSTITUTE FOR COMMUNICABLE DISEASES   (South Africa)

Author keywords

CCHFV; Diagnostic ELISA; Recombinant NP

Indexed keywords

IMMUNOGLOBULIN G ANTIBODY; VIRUS NUCLEOPROTEIN; VIRUS RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY RESPONSE; ARTICLE; BLOOD SAMPLING; CLINICAL ARTICLE; CODON; CONTROLLED STUDY; CRIMEAN CONGO HEMORRHAGIC FEVER; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; GENE SEQUENCE; HUMAN; MOUSE; NAIRO VIRUS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN MODIFICATION; SERODIAGNOSIS; VERO CELL; VIRUS GENE; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, VIRAL; ANTIGENS, VIRAL; BLOTTING, WESTERN; CERCOPITHECUS AETHIOPS; CHILD; CHILD, PRESCHOOL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENE EXPRESSION; HEMORRHAGIC FEVER VIRUS, CRIMEAN-CONGO; HUMANS; IMMUNOGLOBULIN G; INFANT; INFANT, NEWBORN; MICE; NUCLEOPROTEINS; RECOMBINANT PROTEINS; VERO CELLS;

BACTERIA (MICROORGANISMS); CRIMEAN-CONGO HEMORRHAGIC FEVER VIRUS; ESCHERICHIA COLI; HEXAPODA; IXODIDA; MAMMALIA; MUS;

EID: 84855234140     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2011.09.023     Document Type: Article

References (19)

1. Ahmed J., Bouloy M., Ergonul O., Fooks A., Paweska J., Chevalier V., Drosten C., Moormann R., Tordo N., Vatansever Z., Calistri P., Estrada-Pena A., Mirazimi A., Unger H., Yin H., Seitzer U. International network for capacity building for the control of emerging viral vector-borne zoonotic diseases: ARBO-ZOONET. Euro. Surveill. 2009, 14:19160-19163.
2. Bradel-Tretheway B.G., Khen Z., Dewhurst S. Effects of codon-optimization on protein expression by the human herpesvirus 6 and 7 U51 open reading frame. J. Virol. Methods 2003, 111:145-156.
3. Burt F.J., Leman P.A., Abbott J.C., Swanepoel R. Serodiagnosis of Crimean-Congo haemorrhagic fever. Epidemiol. Infect. 1994, 113:551-562.
4. Garcia S., Chinikar S., Coudrier D., Billecocq A., Hooshmand B., Crance J.M., Garin D., Boulov M. Evaluation of a Crimean-Congo hemorrhagic fever virus recombinant antigen expressed by Semliki Forest suicide virus for IgM and IgG antibody detection in human and animal sera collected in Iran. J. Clin. Virol. 2006, 35:154-159.
5. Han J.H., Choi Y.S., Kim W.J., Jeon Y.H., Lee S.K., Lee B.J., Ryu K.S. Codon optimization enhances protein expression of human peptide deformylase in E. coli. Protein Expr. Purif. 2009, 70:224-230.
6. Hoogstraal H. The epidemiology of tick-borne Crimean-Congo hemorrhagic fever in Asia, Europe, and Africa. J. Med. Entomol. 1979, 15:307-417.
7. Ikemura T. Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: a proposal for a synonymous codon choice that is optimal for the E. coli system. J. Mol. Biol. 1981, 151:389-409.
8. Johnson K.M., Elliott L.H., Heymann D.L. Preparation of polyvalent viral immunofluorescent intracellular antigens and use in human serosurveys. J. Clin. Microbiol. 1981, 14:527-529.
9. Li Z., Hong G., Wu Z., Hu B., Xu J., Li L. Optimization of the expression of hepatitis B virus e gene in Pichia pastoris and immunological characterization of the product. J. Biotechnol. 2008, 138:1-8.
10. Maltezou H.C., Andonova L., Andraghetti R., Bouloy M., Ergonul O., Jongejan F., Kalvatchev N., Nichol S., Niedrig M., Platonov A., Thomson G., Leitmeyer K., Zeller H. Crimean-Congo hemorrhagic fever in Europe: current situation calls for preparedness. Euro Surveill. 2010, 15:19504-19508.
11. Marriott A.C., Polyzoni T., Antoniadis A., Nuttall P.A. Detection of human antibodies to Crimean-Congo haemorrhagic fever virus using expressed viral nucleocapsid protein. J. Gen. Virol. 1994, 75:2157-2216.
12. Paweska J.T., Jansen van Vuren P., Swanepoel R. Validation of an indirect ELISA based on a recombinant nucleocapsid protein of Rift Valley fever virus for the detection of IgG antibody in humans. J. Virol. Methods 2007, 146:119-124.
13. Saijo M., Qing T., Niikura M., Maeda A., Ikegami T., Prehaud C., Kurane I., Morikawa S. Recombinant nucleoprotein-based enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies to Crimean-Congo hemorrhagic fever virus. J. Clin. Microbiol. 2002, 40:1587-1591.
14. Saijo M., Qing T., Niikura M., Maeda A., Ikegami T., Sakai K., Prehaud C., Kurane I., Morikawa S. Immunofluorescence technique using HeLa cells expressing recombinant nucleoprotein for detection of immunoglobulin G antibodies to Crimean-Congo hemorrhagic fever virus. J. Clin. Microbiol. 2005, 40:372-375.
15. Sharp P.M., Li W.H. The codon adaptation index a measure of directional synonymous codon usage bias, and its potential applications. Nucleic Acids Res. 1987, 15:1281-1295.
16. Shepherd A.J., Swanepoel R., Gill D.E. Evaluation of enzyme-linked immunosorbent assay and reversed passive hemagglutination for detection of Crimean-Congo hemorrhagic fever virus antigen. J. Clin. Microbiol. 1988, 26:347-353.
17. Simabuco F.M., Tamura R.E., Carromeu C., Farinha-Arcieri L.E., Ventura A.M. Gene optimization leads to robust expression of human respiratory syncytial virus nucleoprotein and phosphoprotein in human cells and induction of humoral immunity in mice. J. Virol. Methods 2009, 158:93-99.
18. Swanepoel R., Shepherd A.J., Leman P.A., Shepherd S.P., McGillivray G.M., Erasmus M.J., Searle L.A., Gill D.E. Epidemiologic and clinical features of Crimean-Congo hemorrhagic fever in southern Africa. Am. J. Trop. Med. Hyg. 1987, 36:120-132.
19. Tang Q., Saijo M., Zhang Y., Asiguma M., Tianshu D., Han L., Shimayi B., Maeda A., Kurane I., Morikawa S. A patient with Crimean-Congo hemorrhagic fever serologically diagnosed by recombinant nucleoprotein-based antibody detection systems. Clin Diagn. Lab. Immunol 2003, 10. 489-491.s.