Optimization of fixed-permeabilized cell monolayers for high throughput micro-neutralizing antibody assays: Application to the zebrafish/viral hemorrhagic septicemia virus (vhsv) model

Journal of Virological Methods

Volumn 193, Issue 2, 2013, Pages 627-632

  Chinchilla, Blanca ^a   Encinas, Paloma ^a   Estepa, Amparo ^b   Coll, Julio ^a   Gomez Casado, Eduardo ^a  

^a INIA   (Spain)

^b MIGUEL HERNÁNDEZ UNIVERSITY   (Spain)

Author keywords

Assay; Fixed permeabilized monolayers; Highthroughput; Neutralizing antibodies; VHSV; Zebrafish

Indexed keywords

MONOCLONAL ANTIBODY; NEUTRALIZING ANTIBODY; SAPONIN; VIRUS PROTEIN;

ANIMAL CELL; ANTIBODY BLOOD LEVEL; ANTIBODY DETECTION; ANTIGEN ANTIBODY COMPLEX; ARTICLE; BLOOD ANALYSIS; CELL DAMAGE; CELL PERMEABILIZATION; CELL SUSPENSION; CONTROLLED STUDY; FLOW CYTOMETRY; HIGH THROUGHPUT MICRONEUTRALIZING ANTIBODY ASSAY; IMMUNOASSAY; INCUBATION TIME; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; VIRAL HEMORRHAGIC SEPTICEMIA; ZEBRA FISH;

ASSAY; FIXED-PERMEABILIZED MONOLAYERS; HIGHTHROUGHPUT; NEUTRALIZING ANTIBODIES; VHSV; ZEBRAFISH;

ANIMALS; ANTIBODIES, NEUTRALIZING; CELL CULTURE TECHNIQUES; HIGH-THROUGHPUT SCREENING ASSAYS; NEUTRALIZATION TESTS; NOVIRHABDOVIRUS; SPECIMEN HANDLING; ZEBRAFISH;

EID: 84882938694     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2013.07.049     Document Type: Article

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