Evaluation of the lightcycler methicillin-resistant Staphylococcus aureus (MRSA) advanced test for detection of MRSA nasal colonization

Journal of Clinical Microbiology

Volumn 51, Issue 9, 2013, Pages 2869-2874

  Yam, W C ^a   Siu, Gilman K H ^a   Ho, P L ^a   Ng, T K ^b   Que, T L ^c   Yip, K T ^c   Fok, Cathie P K ^c   Chen, Jonathan H K ^a   Cheng, Vincent C C ^a   Yuen, K Y ^a  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

^b PRINCESS MARGARET HOSPITAL   (Hong Kong)

^c TUEN MUN HOSPITAL   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL COLONIZATION; BACTERIAL GENE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATE; CLONAL SPECIES; CONTROLLED STUDY; GENE IDENTIFICATION; HIGH TEMPERATURE PROCEDURES; INTERMETHOD COMPARISON; MEC GENE; MEDICAL DEVICE; MELTING POINT; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MOLECULAR CLONING; NONHUMAN; NOSE SMEAR; ORFX GENE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; SINGLE NUCLEOTIDE POLYMORPHISM; TEMPERATURE SENSITIVITY;

BACTERIOLOGICAL TECHNIQUES; CARRIER STATE; HONG KONG; HUMANS; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; MOLECULAR DIAGNOSTIC TECHNIQUES; NASAL MUCOSA; POLYMORPHISM, SINGLE NUCLEOTIDE; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; STAPHYLOCOCCAL INFECTIONS;

EID: 84882786765     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00488-13     Document Type: Article

References (24)

1. Ho P, Yuen K, Yam W, Sai-yin Wong S, Luk W. 1995. Changing patterns of susceptibilities of blood, urinary and respiratory pathogens in Hong Kong. J. Hosp. Infect. 31:305-317.
2. Chambers HF. 1997. Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin. Microbiol. Rev. 10: 781-791.
3. Lina G, Piemont Y, Godail-Gamot F, Bes M, Peter MO, Gauduchon V, Vandenesch F, Etienne J. 1999. Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin. Infect. Dis. 29:1128-1132.
4. Reischl U, Tuohy MJ, Hall GS, Procop GW, Lehn N, Linde H. 2007. Rapid detection of Panton-Valentine leukocidin-positive Staphylococcus aureus by real-time PCR targeting the lukS-PV gene. Eur. J. Clin. Microbiol. Infect. Dis. 26:131-135.
5. Datta R, Huang SS. 2008. Risk of infection and death due to methicillinresistant Staphylococcus aureus in long-term carriers. Clin. Infect. Dis. 47: 176-181.
6. Fang H, Hedin G. 2003. Rapid screening and identification of methicillinresistant Staphylococcus aureus from clinical samples by selective-broth and real-time PCR assay. J. Clin. Microbiol. 41:2894-2899.
7. Reischl U, Frick J, Hoermansdorfer S, Melzl H, Bollwein M, Linde HJ, Becker K, Kock R, Tuschak C, Busch U, Sing A. 2009. Single-nucleotide polymorphism in the SCCmec-orfX junction distinguishes between livestock-associated MRSA CC398 and human epidemic MRSA strains. Euro Surveill. 14(49):pii-19436. http://www.eurosurveillance.org/ViewArticle .aspx?ArticleId-19436.
8. Ho PL, Chuang SK, Choi YF, Lee RA, Lit AC, Ng TK, Que TL, Shek KC, Tong HK, Tse CW, Tung WK, Yung RW. 2008. Community-associated methicillin-resistant and methicillin-sensitive Staphylococcus aureus: skin and soft tissue infections in Hong Kong. Diagn. Microbiol. Infect. Dis. 61:245-250.
9. Enright MC, Day NP, Davies CE, Peacock SJ, Spratt BG. 2000. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J. Clin. Microbiol. 38:1008-1015.
10. Hagen RM, Seegmuller I, Navai J, Kappstein I, Lehn N, Miethke T. 2005. Development of a real-time PCR assay for rapid identification of methicillin-resistant Staphylococcus aureus from clinical samples. Int. J. Med. Microbiol. 295:77-86.
11. Ho PL, Chiu SS, Chan MY, Gan Y, Chow KH, Lai EL, Lau YL. 2012. Molecular epidemiology and nasal carriage of Staphylococcus aureus and methicillin-resistant S. aureus among young children attending day care centers and kindergartens in Hong Kong. J. Infect. 64:500-506.
12. DeLeo FR, Otto M, Kreiswirth BN, Chambers HF. 2010. Communityassociated meticillin-resistant Staphylococcus aureus. Lancet 375:1557-1568.
13. Peterson LR, Liesenfeld O, Woods CW, Allen SD, Pombo D, Patel PA, Mehta MS, Nicholson B, Fuller D, Onderdonk A. 2010. Multicenter evaluation of the LightCycler methicillin-resistant Staphylococcus aureus (MRSA) advanced test as a rapid method for detection of MRSA in nasal surveillance swabs. J. Clin. Microbiol. 48:1661-1666.
14. Louie L, Goodfellow J, Mathieu P, Glatt A, Louie M, Simor AE. 2002. Rapid detection of methicillin-resistant staphylococci from blood culture bottles by using a multiplex PCR assay. J. Clin. Microbiol. 40:2786-2790.
15. Francois P, Pittet D, Bento M, Pepey B, Vaudaux P, Lew D, Schrenzel J. 2003. Rapid detection of methicillin-resistant Staphylococcus aureus directly from sterile or nonsterile clinical samples by a new molecular assay. J. Clin. Microbiol. 41:254-260.
16. Maes N, Magdalena J, Rottiers S, De Gheldre Y, Struelens MJ. 2002. Evaluation of a triplex PCR assay to discriminate Staphylococcus aureus from coagulase-negative staphylococci and determine methicillin resistance from blood cultures. J. Clin. Microbiol. 40:1514-1517.
17. Park SH, Jang YH, Sung H, Kim MN, Kim JS, Park YJ. 2009. Performance evaluation ofBDGeneOhmMRSAPCRassay for detection of nasal colonization of methicillin-resistant Staphylococcus aureus at endemic intensive care units. Korean J. Lab. Med. 29:439-447. (In Korean.)
18. Blanc DS, Basset P, Nahimana-Tessemo I, Jaton K, Greub G, Zanetti G. 2011. High proportion of wrongly identified methicillin-resistant Staphylococcus aureus carriers by use of a rapid commercial PCR assay due to presence of staphylococcal cassette chromosome element lacking the mecA gene. J. Clin. Microbiol. 49:722-724.
19. Laurent C, Bogaerts P, Schoevaerdts D, Denis O, Deplano A, Swine C, Struelens MJ, Glupczynski Y. 2010. Evaluation of the Xpert MRSA assay for rapid detection of methicillin-resistant Staphylococcus aureus from nares swabs of geriatric hospitalized patients and failure to detect a specific SCCmec type IV variant. Eur. J. Clin. Microbiol. Infect. Dis. 29:995-1002.
20. Lindqvist M, Isaksson B, Grub C, Jonassen TO, Hallgren A. 2012. Detection and characterisation of SCCmec remnants in multiresistant methicillin- susceptible Staphylococcus aureus causing a clonal outbreak in a Swedish county. Eur. J. Clin. Microbiol. Infect. Dis. 31:141-147.
21. Ho PL, Lai EL, Chow KH, Chow LS, Yuen KY, Yung RW. 2008. Molecular epidemiology of methicillin-resistant Staphylococcus aureus in residential care homes for the elderly in Hong Kong. Diagn. Microbiol. Infect. Dis. 61:135-142.
22. Jukes L, Mikhail J, Bome-Mannathoko N, Hadfield SJ, Harris LG, El-Bouri K, Davies AP, Mack D. 2010. Rapid differentiation of Staphylococcus aureus, Staphylococcus epidermidis and other coagulase-negative staphylococci and meticillin susceptibility testing directly from growthpositive blood cultures by multiplex real-time PCR. J. Med. Microbiol. 59:1456-1461.
23. Kim JU, Cha CH, An HK, Lee HJ, Kim MN. 2013. Multiplex real-time PCR assay for detection of methicillin-resistant Staphylococcus aureus (MRSA) strains suitable in regions of high MRSA endemicity. J. Clin. Microbiol. 51:1008-1013.
24. Ho PL, Chow KH, Lo PY, Lee KF, Lai EL. 2009. Changes in the epidemiology of methicillin-resistant Staphylococcus aureus associated with spread of the ST45 lineage in Hong Kong. Diagn. Microbiol. Infect. Dis. 64:131-137.