Mucin-type proteins produced in the Trichoplusia ni and Spodoptera frugiperda insect cell lines carry novel O-glycans with phosphocholine and sulfate substitutions

Glycobiology

Volumn 23, Issue 7, 2013, Pages 778-796

  Gaunitz, Stefan ^a   Jin, Chunsheng ^b   Nilsson, Anki ^c   Liu, Jining ^d   Karlsson, Niclas G ^b   Holgersson, Jan ^d  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^b UNIVERSITY OF GOTHENBURG   (Sweden)

^c Novum   (Sweden)

^d SAHLGRENSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

insect cells; mass spectrometry; O glycosylation; phosphocholine; sulfate

Indexed keywords

GALACTURONIC ACID; GLUCURONIC ACID; GLYCAN DERIVATIVE; HEXOSE; HEXURONIC ACID; HYBRID PROTEIN; MUCIN TYPE PROTEIN; N ACETYLGALACTOSAMINE; OLIGOSACCHARIDE; P SELECTIN GLYCOPROTEIN LIGAND 1; PHOSPHORYLCHOLINE; PROTEIN; SULFATE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; CARBOHYDRATE ANALYSIS; CONTROLLED STUDY; CULTURE MEDIUM; GEL FILTRATION CHROMATOGRAPHY; INSECT; INSECT CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN PURIFICATION; SPODOPTERA FRUGIPERDA; TANDEM MASS SPECTROMETRY; TRICHOPLUSIA NI; WESTERN BLOTTING;

INSECT CELLS; MASS SPECTROMETRY; O-GLYCOSYLATION; PHOSPHOCHOLINE; SULFATE;

ACETYLGALACTOSAMINE; ANIMALS; CELL LINE; GLYCOSYLATION; HEXURONIC ACIDS; MEMBRANE GLYCOPROTEINS; MICE; MOTHS; MUCINS; PHOSPHORYLCHOLINE; POLYSACCHARIDES; RECOMBINANT FUSION PROTEINS; SF9 CELLS; SPODOPTERA; SULFATES;

EID: 84882778713     PISSN: 09596658     EISSN: 14602423     Source Type: Journal    
DOI: 10.1093/glycob/cwt015     Document Type: Article

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