Simultaneous detection of West Nile and Japanese encephalitis virus RNA by duplex TaqMan RT-PCR

Journal of Virological Methods

Volumn 193, Issue 2, 2013, Pages 554-557

  Barros, Silvia C ^a   Ramos, Fernanda ^a   Zé Zé, Líbia ^b   Alves, Maria J ^b   Fagulha, Teresa ^a   Duarte, Margarida ^a   Henriques, Margarida ^a   Luís, Tiago ^a   Fevereiro, Miguel ^a  

^a INIAV   (Portugal)

^b NATIONAL INSTITUTE OF HEALTH DR RICARDO JORGE   (Portugal)

Author keywords

Diagnosis; Duplex RT PCR; JEV; WNV

Indexed keywords

VIRUS RNA;

ARTICLE; CONTROLLED STUDY; GENE DOSAGE; IN VITRO STUDY; JAPANESE ENCEPHALITIS VIRUS; KUNJIN VIRUS; NONHUMAN; NS2A GENE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SENSITIVITY ANALYSIS; VIRUS GENE; WEST NILE FLAVIVIRUS;

DIAGNOSIS; DUPLEX RT-PCR; JEV; WNV;

ENCEPHALITIS VIRUS, JAPANESE; ENCEPHALITIS, JAPANESE; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; WEST NILE FEVER; WEST NILE VIRUS;

EID: 84882685381     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2013.07.025     Document Type: Article

References (26)

1. Bakonyi T., Hubalek Z., Rudolf I., Nowotny N. Novel flavivirus or new lineage of West Nile virus, central Europe. Emerging Infectious Diseases 2005, 11:225-231.
2. Botha E.M., Markotter W., Wolfaardt M., Paweska J.T., Swanepoel R., Palacios G., Nel L.H., Venter M. Genetic determinants of virulence in pathogenic lineage 2 West Nile virus strains. Emerging Infectious Diseases 2008, 14:222-230.
3. Buckley A., Dawson A., Moss S.R., Hinsley S.A., Bellamy P.E., Gould E.A. Serological evidence of West Nile virus, USUTU virus and Sindbis virus infection in birds in the UK. Journal of General Virology 2003, 84:2807-2817.
4. De Filette M., Ulbert S., Diamond M., Sanders N. Recent progress in West Nile virus diagnosis and vaccination. Veterinary Research 2012, 43:16.
5. Del Amo J., Sotelo E., Fernández-Pinero J., Gallardo C., Llorente F., Agvero M., Jiménez-Clavero M.A. A novel quantitative multiplex real-time RT-PCR for the simultaneous detection and differentiation of West Nile virus lineages 1 and 2, and of USUTU virus. Journal of Virological Methods 2013, 189:321-327.
6. Eiden M., Vina-Rodriguez A., Hoffmann B., Ziegler U., Groschup H.M. Two new real-time quantitative reverse transcription polymerase chain reaction assays with unique target sites for the specific and sensitive detection of lineages 1 and 2 West Nile virus strains. Journal of Veterinary Diagnostic Investigation 2010, 22:748-753.
7. Gamino V., Gutiérrez-Guzmán A.-V., Fernández-de-Mera I.G., Ortíz J.-A., Durán-Martín M., de la Fuente J., Gortázar C., Höfle U. Natural Bagaza virus infection in game birds in southern Spain. Veterinary Research 2012, 43:65.
8. Ghosh D., Basu A. Japanese encephalitis - a pathological and clinical perspective. PLoS Neglected Tropical Diseases 2009, 3:e437.
9. Gould E.A., de Lamballerie X., Zanotto P.M., Holmes E.C. Origins, evolution, and vector/host coadaptations within the genus flavivirus. Advances in Virus Research 2003, 59:277-314.
10. Jimenez-Clavero M.A., Aguero M., Rojo G., Gomez-Tejedor C. A new fluorogenic real-time RT-PCR assay for detection of lineage 1 and lineage 2 West Nile viruses. Journal of Veterinary Diagnostic Investigation 2006, 18:459-462.
11. Kramer L.D., Styer L.M., Ebel G.D. A global perspective on the epidemiology of West Nile virus. Annual Review of Entomology 2008, 53:61-81.
12. Lanciotti R.S., Roehrig J., Deubel V., Smith J., Parker M., Steele K., Crise B., Volpe K.E., Crabtree M.B., Scherret J.H., Hall R.A., MacKenzie J.S., Cropp C.B., Panigrahy B., Ostlund E., Schmitt B., Malkinsin M., Banet C., Weissman J., Komar N., Savage H.M., Stone W., McNamara T., Gubler D.J. Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States. Science 1999, 286:2333-2337.
13. Lanciotti R.S., Kerst A., Nasci R., Godsey M., Mitchell C., Savage H., Komar N., Panella N.A., Allen B.C., Volpe K.E., David B.S., Roehrig J.T. Rapid detection of West Nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCR assay. Journal of Clinical Microbiology 2000, 38:4066-4071.
14. Linke S., Ellerbrok H., Niedrig M., Nitsche A., Pauli G. Detection of West Nile virus lineages 1 and 2 by real-time PCR. Journal of Virological Methods 2007, 146:355-358.
15. Linke S., Mackay W., Scott C., Wallace P., Niedrig M. Second external quality assessment of the molecular diagnostic of West Nile virus: are there improvements towards the detection of WNV?. Journal of Clinical Virology 2011, 52:257-260.
16. Mackay I.M., Arden K.E., Nitsche A. Real-time PCR in virology. Nucleic Acids Research 2002, 30:1292-1305.
17. Pfaffl M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research 2001, 29:e45.
18. Platonov A., Rossi G., Karan L., Mironov K., Busani L., Rezza G. Does the Japanese encephalitis virus (JEV) represent a threat for human health in Europe? Detection of JEV RNA sequences in birds collected in Italy. Euro Surveillance 2012, 17(32). pii:20241.
19. Ravanini P., Huhtamo E., Ilaria V., Crobu M.G., Nicosia A.M., Servino L., Rivasi F., Allegrini S., Miglio U., Magri A., Minisini R., Vapalahti O., Boldorini R. Japanese encephalitis virus RNA detected in Culex pipiens mosquitoes in Italy. Eurosurveillance 2012, 17:2.
20. Shirato K., Mizutani T., Kariwa H., Takashima I. Discrimination of Wst Nile virus and Japanese encephalitis virus strains using RT-PCR RFLP analysis. Microbiology and Immunology 2003, 47:439-445.
21. Shirato K., Miyoshi H., Kariwa H., Takashima I. Detection of West Nile virus and Japanese encephalitis virus using real-time PCR with a probe common to both viruses. Journal of Virological Methods 2005, 126:119-125.
22. Solomon T., Ooi M.H., Beasley D.W., Mallewa M. West Nile encephalitis. BMJ 2003, 326:865-869.
23. Vásquez A., Jiménez-Clavero M.A., Franco L., Donoso-Mantke O., Sambri V., Niedrig M., Zeller H., Tenorio A. Usutu vírus-potential risk of human disease in Europe. Eurosurveillance 2011, 16(31). pii:19935.
24. Vaughn D.W., Hoke C.H. The epidemiology of Japanese encephalitis: prospects for prevention. Epidemiologic Reviews 1992, 14:197-221.
25. Venter M., Human S., Zaayman D., Gerdes G.H., Williams J., Steyl J., Leman P.A., Paweska J.T., Setzkorn H., Rous G., Murray S., Parker R., Donellan C., Swanepoel R. Lineage 2 West Nile virus as cause of fatal neurologic disease in horses. South Africa - Emerging infectious diseases 2009, 15:877-884.
26. Yeh J.Y., Lee J.H., Seo H.J., Park J.Y., Moon J.S., Cho I.S., Lee J.B., Park S.Y., Song C.S., Choi I.S. Fast duplex one-step reverse transcriptase PCR for rapid differential detection of West Nile and Japanese encephalitis viruses. Journal of Clinical Microbiology 2010, 48:4010-4014.