Comparison of real-time RT-PCR assays for hepatitis E virus RNA detection

Journal of Clinical Virology

Volumn 58, Issue 1, 2013, Pages 36-40

  Mokhtari, Camelia ^a   Marchadier, Eric ^a   Haïm Boukobza, Stephanie ^a,c,d   Jeblaoui, Asma ^a   Tessé, Sophie ^b   Savary, Jeanine ^a   Roque Afonso, Anne Marie ^a,c,d  

^a HÔpital Paul Brousse   (France)

^b SERVICE D ENDOCRINOLOGIE   (France)

^c UNIVERSITÉ PARIS SACLAY   (France)

^d INSERM   (France)

Author keywords

HEV diagnosis; HEV genotypes; Real time RT PCR assay

Indexed keywords

VIRUS RNA;

ARTICLE; COMPARATIVE STUDY; GENE REPLICATION; GENETIC VARIABILITY; GENOTYPE; HEPATITIS E VIRUS; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS DETECTION; VIRUS LOAD; VIRUS REPLICATION;

EID: 84881553333     PISSN: 13866532     EISSN: 18735967     Source Type: Journal    
DOI: 10.1016/j.jcv.2013.06.038     Document Type: Article

References (13)

1. Kamar N., Bendall R., Legrand-Abravanel F., Xia N.S., Ijaz S., Izopet J., et al. Hepatitis E. Lancet 2012, 379:2477-2488.
2. Tei S., Kitajima N., Takahashi K., Mishiro S. Zoonotic transmission of hepatitis E virus from deer to human beings. Lancet 2003, 362:371-373.
3. Colson P., Borentain P., Queyriaux B., Kaba M., Moal V., Gallian P., et al. Pig liver sausage as a source of hepatitis E virus transmission to humans. J Infect Dis 2010, 202:825-834.
4. Meng X.J. Recent advances in hepatitis E virus. J Viral Hepat 2010, 17:153-161.
5. Baylis S.A., Hanschmann K.M., Blumel J., Nubling C.M. Standardization of hepatitis E virus (HEV) nucleic acid amplification technique-based assays: an initial study to evaluate a panel of HEV strains and investigate laboratory performance. J Clin Microbiol 2011, 49:1234-1239.
6. Abravanel F., Sandres-Saune K., Lhomme S., Dubois M., Mansuy J.M., Izopet J. Genotype 3 diversity and quantification of hepatitis e virus RNA. J Clin Microbiol 2012, 50:897-902.
7. Jothikumar N., Cromeans T.L., Robertson B.H., Meng X.J., Hill V.R. A broadly reactive one-step real-time RT-PCR assay for rapid and sensitive detection of hepatitis E virus. J Virol Methods 2006, 131:65-71.
8. Rolfe K.J., Curran M.D., Mangrolia N., Gelson W., Alexander G.J., L'Estrange M., et al. First case of genotype 4 human hepatitis E virus infection acquired in India. J Clin Virol 2010, 48:58-61.
9. Mansuy J.M., Peron J.M., Abravanel F., Poirson H., Dubois M., Miedouge M., et al. Hepatitis E in the south west of France in individuals who have never visited an endemic area. J Med Virol 2004, 74:419-424.
10. Colson P., Coze C., Gallian P., Henry M., De Micco P., Tamalet C., France Transfusion-associated hepatitis E. Emerg Infect Dis 2007, 13:648-649.
11. Kaba M., Davoust B., Marie J.L., Barthet M., Henry M., Tamalet C., et al. Frequent transmission of hepatitis E virus among piglets in farms in Southern France. J Med Virol 2009, 81:1750-1759.
12. Cooper K., Huang F.F., Batista L., Rayo C.D., Bezanilla J.C., Toth T.E., et al. Identification of genotype 3 hepatitis E virus (HEV) in serum and fecal samples from pigs in Thailand and Mexico, where genotype 1 and 2 HEV strains are prevalent in the respective human populations. J Clin Microbiol 2005, 43:1684-1688.
13. Ward P., Poitras E., Leblanc D., Letellier A., Brassard J., Plante D., et al. Comparative analysis of different TaqMan real-time RT-PCR assays for the detection of swine Hepatitis E virus and integration of Feline calicivirus as internal control. J Appl Microbiol 2009, 106:1360-1369.