Analysis of a pannexin 2-pannexin 1 chimeric protein supports divergent roles for pannexin C-termini in cellular localization

Cell Communication and Adhesion

Volumn 20, Issue 3-4, 2013, Pages 73-79

  Wicki Stordeur, Leigh E ^a   Boyce, Andrew K J ^a   Swayne, Leigh Anne ^a,b  

^a UNIVERSITY OF VICTORIA   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Chimer; Endolysosomes; Pannexin 1; Pannexin 1 C terminus; Pannexin 2; Plasma membrane targeting; Trafficking

Indexed keywords

CHIMERIC PROTEIN; PANNEXIN 1; PANNEXIN 2; SOMATOMEDIN B RECEPTOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BIOTINYLATION; CELLULAR DISTRIBUTION; IMMUNOHISTOCHEMISTRY; MICROSCOPY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN LOCALIZATION; WESTERN BLOTTING;

ANIMALS; CELLS, CULTURED; CONNEXINS; MICE; NERVE TISSUE PROTEINS; PROTEIN TRANSPORT;

EID: 84881412454     PISSN: 15419061     EISSN: 15435180     Source Type: Journal    
DOI: 10.3109/15419061.2013.791681     Document Type: Article

References (24)

1. Ambrosi C, Gassmann O, Pranskevich JN, Boassa D, Smock A, Wang J, Dahl G, Steinem C, Sosinsky GE (2010). Pannexin1 and Pannexin2 channels show quaternary similarities to connexons and different oligomerization numbers from each other. J Biol Chem. 285:24420-24431.
2. Baranova A, Ivanov D, Petrash N, Pestova A, Skoblov M, Kelmanson I, Shagin D, Nazarenko S, Geraymovych E, Litvin O, Tiunova A, Born TL, Usman N, Staroverov D, Lukyanov S, Panchin Y (2004). The mammalian pannexin family is homologous to the invertebrate innexin gap junction proteins. Genomics. 83:706-716.
3. Bargiotas P, Krenz A, Hormuzdi SG, Ridder DA, Herb A, Barakat W, Penuela S, von Engelhardt J, Monyer H, Schwaninger M (2011). Pannexins in ischemia-induced neurodegeneration. Proc Natl Acad Sci USA 108:20772-20777.
4. Bhalla-Gehi R, Penuela S, Churko JM, Shao Q, Laird DW (2010). Pannexin1 and pannexin3 delivery, cell surface dynamics, and cytoskeletal interactions. J Biol Chem. 285:9147-9160.
5. Boassa D, Ambrosi C, Qiu F, Dahl G, Gaietta G, Sosinsky G (2007). Pannexin1 channels contain a glycosylation site that targets the hexamer to the plasma membrane. J Biol Chem. 282:31733-31743.
6. Bond SR, Naus CC (2012). RF-Cloning.org: an online tool for the design of restriction-free cloning projects. Nucleic Acids Res. 40:W209-213.
7. Bruzzone R, Hormuzdi SG, Barbe MT, Herb A, Monyer H (2003). Pannexins, a family of gap junction proteins expressed in brain. Proc Natl Acad Sci USA. 100:13644-13649.
8. Bruzzone R, White TW, Paul DL (1994). Expression of chimeric connexins reveals new properties of the formation and gating behavior of gap junction channels. J Cell Sci. 107:955-967.
9. Chekeni FB, Elliott MR, Sandilos JK, Walk SF, Kinchen JM, Lazarowski ER, Armstrong AJ, Penuela S, Laird DW, Salvesen GS, Isakson BE, Bayliss DA, Ravichandran KS (2010). Pannexin 1 channels mediate ' fnd-me' signal release and membrane permeability during apoptosis. Nature. 467:863-867.
10. Gehi R, Shao Q, Laird DW (2011). Pathways regulating the Trafficking and turnover of pannexin1 protein and the role of the C-terminal domain. J Biol Chem. 286:27639-27653.
11. Ghosh P, Dahms NM, Kornfeld S (2003). Mannose 6-phosphate receptors: new twists in the tale. Nat Rev Mol Cell Biol. 4:202-212.
12. Hayashi T, Thomas GM, Huganir RL (2009). Dual palmitoylation of NR2 subunits regulates NMDA receptor Trafficking. Neuron. 64:213-226.
13. Lai CP, Bechberger JF, Naus CC (2009). Pannexin2 as a novel growth regulator in C6 glioma cells. Oncogene 28: 4402-4408.
14. Lai CP, Bechberger JF, Thompson RJ, MacVicar BA, Bruzzone R, Naus CC (2007). Tumor-suppressive effects of pannexin 1 in C6 glioma cells. Cancer Res. 67:1545-1554.
15. Olk S, Zoidl G, Dermietzel R (2009). Connexins, cell motility, and the cytoskeleton. Cell Motil Cytoskeleton. 66:1000-1016.
16. Penuela S, Bhalla R, Gong X-Q, Cowan KN, Celetti SJ, Cowan BJ, Bai D, Shao Q, Laird DW (2007). Pannexin 1 and pannexin 3 are glycoproteins that exhibit many distinct characteristics from the connexin family of gap junction proteins. J Cell Sci. 120:3772-3783.
17. Penuela S, Bhalla R, Nag K, Laird DW (2009). Glycosylation regulates pannexin intermixing and cellular localization. Mol Biol Cell. 20:4313-4323.
18. Penuela S, Gehi R, Laird DW (2012). The biochemistry and function of pannexin channels. Biochimica et Biophysica Acta. 1828:15-22.
19. Sandilos JK, Chiu YH, Chekeni FB, Armstrong AJ, Walk SF, Ravichandran KS, Bayliss DA (2012). Pannexin 1, an ATP release channel, is activated by caspase cleavage of its pore-associated C terminal autoinhibitory region. J Biol Chem. 287:11303-11311.
20. Swayne LA, Bourinet E (2008). Voltage-gated calcium channels in chronic pain: emerging role of alternative splicing. Pfugers Arch. 456:459-466.
21. Swayne LA, Sorbara CD, Bennett SA (2010). Pannexin 2 is expressed by postnatal hippocampal neural progenitors and modulates neuronal commitment. J Biol Chem. 285: 24977-24986.
22. Vanden Abeele F, Bidaux G, Gordienko D, Beck B, Panchin YV, Baranova AV, Ivanov DV, Skryma R, Prevarskaya N (2006). Functional implications of calcium permeability of the channel formed by pannexin 1. J Cell Biol. 174:535-546.
23. Wicki-Stordeur LE, Dzugalo AD, Swansburg RM, Suits JM, Swayne LA (2012). Pannexin 1 regulates postnatal neural stem and progenitor cell proliferation. Neural Dev. 7:11.
24. Yatani A, Bahinski A, Wakamori M, Tang S, Mori Y, Kobayashi T, Schwartz A (1994). Alteration of channel characteristics by exchange of pore-forming regions between two structurally related Ca2 + channels. Mol Cell Biochem. 140:93-102.