Bat white-nose syndrome: A real-time taqman polymerase chain reaction test targeting the intergenic spacer region of geomyces destructans

Mycologia

Volumn 105, Issue 2, 2013, Pages 253-259

  Muller, Laura ^a   Lorch, Jeffrey ^b   Lindner, Daniel ^c   O'Connor, Michael ^d   Gargas, Andrea ^e   Blehert, David ^a  

^a NATIONAL WILDLIFE HEALTH CENTER   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

^c Center for Forest Mycology Research   (United States)

^d UNIVERSITY OF WISCONSIN MADISON   (United States)

^e Symbiology LLC   (United States)

Author keywords

Diagnostic test; IGS; Pathogen; PCR; Wildlife disease

Indexed keywords

BAT; FUNGAL DISEASE; FUNGUS; GENOME; HIBERNATION; HISTOPATHOLOGY; POLYMERASE CHAIN REACTION;

FUNGAL DNA; RIBOSOMAL SPACER DNA; SPACER DNA;

ANIMAL; ANIMAL DISEASE; ARTICLE; ASCOMYCETES; BAT; CHEMISTRY; CLASSIFICATION; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; MYCOSIS; PHYLOGENY; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SKIN; SPECIES DIFFERENCE; TIME; UNITED STATES;

ANIMALS; ASCOMYCOTA; CHIROPTERA; DNA, FUNGAL; DNA, INTERGENIC; DNA, RIBOSOMAL SPACER; MYCOSES; PHYLOGENY; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SKIN; SPECIES SPECIFICITY; TIME FACTORS; UNITED STATES;

EID: 84881350661     PISSN: 00275514     EISSN: 15572536     Source Type: Journal    
DOI: 10.3852/12-242     Document Type: Article

References (24)

1. Anderson JB, Stasovski E. 1992. Molecular phylogeny of northern hemisphere species of Armillaria. Mycologia 84:505-516, doi:10.2307/3760315
2. Blehert DS, Hicks AC, Behr M, Meteyer CU, Berlowski-Zier BM, Buckles EL, Coleman JTH, Darling SR, Gargas A, Niver R, Okoniewski JC, Rudd RJ, Stone WB. 2009. Bat white-nose syndrome: an emerging fungal pathogen? Science 323:227, doi:10.1126/science.1163874
3. Boyle DG, Boyle DB, Olsen V, Morgan JA, Hyatt AD. 2004. Rapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using real-time TaqMan PCR assay. Dis Aquat Organ 60:141-148, doi:10.3354/dao060141
4. Boyles JG, Cryan PM, McCracken GF, Kunz TH. 2011. Economic importance of bats in agriculture. Science 332:41-42, doi:10.1126/science.1201366
5. Chaturvedi S, Rudd RJ, Davis A, Victor TR, Li X, Appler KA, Rajkumar SS, Chaturvedi V. 2011. Rapid real-time PCR assay for culture and tissue identification of Geomyces destructans: the etiologic agent of bat geomycosis 258 MYCOLOGIA (white-nose syndrome). Mycopathologia 172:247-256, doi:10.1007/s11046-011-9435-5
6. Gargas A, Trest MT, Christensen M, Volk TJ, Blehert DS. 2009. Geomyces destructans sp. nov. associated with bat white-nose syndrome. Mycotaxon 108:147-154, doi:10.5248/108.147
7. Hillis DM, Dixon MT. 1991. Ribosomal DNA: molecular evolution and phylogenetic inference. Q Rev Biol 66: 411-453, doi:10.1086/417338
8. Jackson CJ, Barton RC, Evans EGV. 1999. Species identification and strain differentiation of dermatophyte fungi by analysis of ribosomal-DNA intergenic spacer regions. J Clin Microbiol 37:931-936.
9. King JL, Guidry C. 2004. Müller cell production of insulin-like growth factor-binding proteins in vitro: modulation with phenotype and growth factor stimulation. InvestOphthalmol Vis Sci 45:4535-4542.
10. Kunz TH, Braun de Torrez EC, Bauer DM, Lobova TA, Fleming TH. 2011. Ecosystem services provided by bats. In: Ostfeld RS, Schlesinger WH, eds. The year in ecology and conservation (Annals of the New York Academy of Sciences). New York: Wiley-Blackwell. p 1-38.
11. Lindner DL, Banik MT. 2009. Effects of cloning and root-tip size on observations of fungal ITS sequences from Picea glauca roots. Mycologia 101:157-165, doi:10.3852/08-034
12. Lindner DL, Gargas A, Lorch JM, Banik MT, Glaeser J, Kunz TH, Blehert DS. 2011. DNA-based detection of the fungal pathogen Geomyces destructans in soils from bat hibernacula. Mycologia 103:241-246, doi:10.3852/10-262
13. Ló pez-Flores I, Robles F, Vlencia JM, Grau A, Villalba A, de la Herrán R, Garrido-Ramos MA, Ruiz-Rejón C, Ruiz- Rejón M, Navas JI. 2008. Detection of Marteilia refringens using nested PCR and in situ hybridization in Chamelea gallina from the Balearic Islands (Spain). Dis Aquat Organ 82:79-87, doi:10.3354/dao01966
14. Lorch JM, Gargas A, Meteyer CU, Berlowski-Zier BM, Green DE, Shearn-Bochsler V, Thomas NJ, Blehert DS. 2010. Rapid polymerase chain reaction diagnosis of whitenose syndrome in bats. J Vet Diagn Invest 22:224-230, doi:10.1177/104063871002200208
15. Lorch JM, Lindner DL, Gargas A, Muller LK, Minnis AM, Blehert DS. 2013. A culture-based survey of fungi in soil from bat hibernacula in the eastern United States and its implications for detection of Geomyces destructans, the causal agent of bat white-nose syndrome. Mycologia 105:237-252, doi:10.3852/12-207
16. Lorch JM, Meteyer CU, Behr MJ, Boyles JG, Cryan PM, Hicks AC, Ballmann AE, Coleman JTH, Redell D, Reeder DM, Blehert DS. 2011. Experimental infection of bats with Geomyces destructans cause white-nose syndrome. Nature 480:376-378, doi:10.1038/nature10590
17. Meteyer CU, Buckles EL, Blehert DS, Hicks AC, Green DE, Shearn-Bochsler V, Thomas NJ, Gargas A, Behr MJ. 2009. Histopathologic criteria to confirm white-nose syndrome in bats. J Vet Diagn Invest 21:411-414, doi:10.1177/104063870902100401
18. Radford SA, Johnson EM, Leeming JP, Millar MR, Cornish JM, Foot ABM, Warnock DW. 1998. Molecular epidemiological study of Aspergillus fumigatus in a bone marrow transplantation unit by PCR amplification of ribosomal intergenic spacer sequences. J Clin Microbiol 36:1294-1299.
19. Suarez MB, Walsh K, Boonham N, O'Neill T, Pearson S, Barker I. 2005. Development of real-time PCR (Taq- Man) assays for the detection and quantification of Botrytis cinerea in planta. Plant Physiol Biochem 43: 890-899.
20. Turner GG, Reeder DM, Coleman JTH. 2011. A five-year assessment of mortality and geographic spread of white-nose syndrome in North American bats and a look to the future. Bat Res News 52:13-27.
21. Turner GG, Reeder DM. 2009. Update of white-nose syndrome in bats, September 2009. Bat Res News 50:47-53.
22. Warnecke L, Turner JM, Bollinger TK, Lorch JM, Misra V, Cryan PM, Wibbelt G, Blehert DS, Willis CKR. 2012. Inoculation of bats with European Geomyces destructans supports the novel pathogen hypothesis for the origin of white-nose syndrome. Proc Natl Acad Sci USA. 109: 6999-7003, doi:10.1073/pnas.1200374109
23. White TJ, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M, Gelfand D, Sninsky J, White T, eds. PCR protocols: a guide to methods and applications. San Diego: Academic Press. p 315-322.
24. Williamson EC, Speers D, Arthur IH, Harnett G, Ryan G, Inglis TJ. 2001. Molecular epidemiology of Scedosporium apiospermum infection determined by PCR amplification of ribosomal intergenic spacer sequences in patients with chronic lung disease. J Clin Microbiol 39: 47-50, doi:10.1128/JCM.39.1.47-50.2001