A simple methodology to assess endolysosomal protease activity involved in antigen processing in human primary cells

BMC Cell Biology

Volumn 14, Issue 1, 2013, Pages

  Vaithilingam, Archana ^a,b   Lai, Nicole Y ^a   Duong, Ellen ^a   Boucau, Julie ^a,b   Xu, Yang ^a   Shimada, Mariko ^a   Gandhi, Malini ^a   Le Gall, Sylvie ^a,b  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Antigen processing; Cathepsins; Endolysosome; HIV; Mass spectrometry; MHC; Primary cells; Proteases; Protein degradation; T cell epitope production

Indexed keywords

CATHEPSIN B; CATHEPSIN D; CATHEPSIN K; CATHEPSIN S; LYSOSOME ASSOCIATED MEMBRANE PROTEIN 1; PEPTIDES AND PROTEINS; PROTEIN B27KK10; PROTEIN B57KF11; PROTEINASE; UNCLASSIFIED DRUG; ANTIGEN; CATHEPSIN; LYSOSOMAL PROTEINS; PEPTIDE HYDROLASE; PROTEIN;

ACIDITY; ANTIGEN PRESENTATION; ARTICLE; CENTRIFUGATION; CONTROLLED STUDY; ENDOLYSOSOME; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SPECIFICITY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS; LYSOSOME; MASS SPECTROMETRY; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIMARY CELL CULTURE; PROTEIN DEGRADATION; PROTEIN DETERMINATION; PROTEIN FUNCTION; PROTEIN PROCESSING; AMINO ACID SEQUENCE; CELL CULTURE; CYTOLOGY; ENZYMOLOGY; EVALUATION STUDY; IMMUNOLOGY; METABOLISM; MOLECULAR GENETICS; MONONUCLEAR CELL; PH; PHYSIOLOGY; PROCEDURES;

MUS;

AMINO ACID SEQUENCE; ANTIGEN PRESENTATION; ANTIGENS; CATHEPSINS; CELLS, CULTURED; HUMANS; HYDROGEN-ION CONCENTRATION; LEUKOCYTES, MONONUCLEAR; LYSOSOMES; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDE HYDROLASES; PROTEINS;

EID: 84881126449     PISSN: None     EISSN: 14712121     Source Type: Journal    
DOI: 10.1186/1471-2121-14-35     Document Type: Article

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