Isolation and analysis of DNA derived from nucleosome-free regions

Methods in Molecular Biology

Volumn 977, Issue , 2013, Pages 35-51

  Murtha, Matthew ^a   Wang, Yatong ^a   Basilico, Claudio ^a   Dailey, Lisa ^a  

^a NONE

Author keywords

Chr omatin; Cis Regulator y modules; Enhancers; Functional assay; Nucleosome free regions; Pr omoters; Transcription

Indexed keywords

DNA; TRANSCRIPTION FACTOR; FIXATIVE; FORMALDEHYDE;

ARTICLE; DNA DETERMINATION; DNA ISOLATION; ENHANCER REGION; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENOME ANALYSIS; MAMMAL; NONHUMAN; NUCLEOSOME; PRIORITY JOURNAL; PROMOTER REGION; ANIMAL; CELL CULTURE; CELL NUCLEUS; CHEMISTRY; CHROMATIN IMMUNOPRECIPITATION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; TISSUE FIXATION;

ANIMALS; CELL NUCLEUS; CELLS, CULTURED; CHROMATIN IMMUNOPRECIPITATION; CLONING, MOLECULAR; DNA; FIXATIVES; FORMALDEHYDE; HUMANS; NUCLEOSOMES; POLYMERASE CHAIN REACTION; TISSUE FIXATION;

MLCS; MLOWN;

EID: 84881019325     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-284-1_4     Document Type: Article

References (5)

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2. Xi H, Shulha HP, Lin JM et al (2007) Identification and characterization of cell type-specific and ubiquitous chromatin regulatory structures in the human genome. PLoS Genet 3(8): 1377-1388
3. Yaragatti M, Basilico C, Dailey L. (2008) Identification of active transcriptional regulatory modules by the functional assay of DNA from nucleosome-free regions. Genome Res 18(6): 930-938
4. Giresi PG, Kim J, McDanielle RM et al (2007) FAIRE (Formaldehyde-Assisted Isolation of Regulatory Elements) isolates active regulatory elements from human chromatin. Genome Res 17(6): 877-885
5. Song L, Zhang Z, Grasfeder LL et al (2011) Open chromatin defined by DNaseI and FAIRE identifies regulatory elements that shape cell-type identity. Genome Res 21(10): 1757-1767