Heavy methyl-SILAC labeling coupled with liquid chromatography and high-resolution mass spectrometry to study the dynamics of site-specific histone methylation

Methods in Molecular Biology

Volumn 977, Issue , 2013, Pages 299-313

  Cao, Xing Jun ^a   Zee, Barry M ^a   Garcia, Benjamin A ^a  

^a NONE

Author keywords

Dynamics; Heavy methionine; Histone; Mass spectrometry; Methylation; Quantitation; SILAC

Indexed keywords

METHIONINE; ACID ANHYDRIDE; CARBON; HISTONE; PROPIONIC ACID ANHYDRIDE; PROPIONIC ACID DERIVATIVE; TRYPSIN;

ARTICLE; CELL LABELING; HISTONE METHYLATION; HUMAN; HUMAN CELL; ISOTOPE LABELING; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR DYNAMICS; PRIORITY JOURNAL; PROTEIN PURIFICATION; ANIMAL; CELL FRACTIONATION; CELL LINE; CELL NUCLEUS; CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; METHYLATION; PROTEIN DEGRADATION; PROTEIN PROCESSING; REVERSED PHASE LIQUID CHROMATOGRAPHY; TANDEM MASS SPECTROMETRY;

ANHYDRIDES; ANIMALS; CARBON ISOTOPES; CELL FRACTIONATION; CELL LINE; CELL NUCLEUS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; HISTONES; HUMANS; ISOTOPE LABELING; METHIONINE; METHYLATION; PROPIONATES; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEOLYSIS; TANDEM MASS SPECTROMETRY; TRYPSIN;

MLCS; MLOWN;

EID: 84881010295     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-284-1_24     Document Type: Article

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