Preparation of cell lines for single-cell analysis of transcriptional activation dynamics

Methods in Molecular Biology

Volumn 977, Issue , 2013, Pages 249-258

  Rafalska Metcalf, Ilona U ^a   Janicki, Susan M ^a  

^a NONE

Author keywords

Chromatin; Live cell imaging; Stable cell lines; Transcription; Transgene array

Indexed keywords

ARTICLE; CELL LINE; GENE CONTROL; MOLECULAR DYNAMICS; MOLECULAR IMAGING; PRIORITY JOURNAL; REGULATORY MECHANISM; SINGLE CELL ANALYSIS; TRANSCRIPTION INITIATION; ANIMAL; CHEMISTRY; CULTURE TECHNIQUE; GENETIC TRANSFECTION; HUMAN; METHODOLOGY; REPORTER GENE; TRANSGENE;

CALCIUM PHOSPHATE;

ANIMALS; CALCIUM PHOSPHATES; CELL CULTURE TECHNIQUES; CELL LINE; GENES, REPORTER; HUMANS; SINGLE-CELL ANALYSIS; TRANSCRIPTIONAL ACTIVATION; TRANSFECTION; TRANSGENES;

MLCS; MLOWN;

EID: 84880999878     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-284-1_20     Document Type: Article

References (11)

1. Rafalska-Metcalf I.U., Janicki S.M. (2007) Show and tell: visualizing gene expression in living cells. J Cell Sci 120: 2301-2307
2. Wu B, Piatkevich KD, Lionnet T, Singer R.H., Verkhusha V.V. (2011) Modern fluorescent proteins and imaging technologies to study gene expression, nuclear localization, and dynamics. Curr Opin Cell Biol 23: 310-317
3. Darzacq X, Yao J, Larson D.R., Causse SZ, Bosanac L, de Turris V, Ruda VM, Lionnet T, Zenklusen D., Guglielmi B, Tjian R, Singer R.H. (2009) Imaging transcription in living cells. Annu Rev Biophys 38: 173-196
4. Belmont AS (2001) Visualizing chromosome dynamics with GFP. Trends Cell Biol 11: 250-257
5. Keryer-Bibens C., Barreau C, Osborne H.B. (2008) Tethering of proteins to RNAs by bac-teriophage proteins. Biol Cell 100: 125-138
6. Paige JS, Wu KY, Jaffrey S.R. (2011) RNA mimics of green fluorescent protein. Science 333: 642-646
7. Tsukamoto T, Hashiguchi N, Janicki S.M., Tumbar T., Belmont AS, Spector D.L. (2000) Visualization of gene activity in living cells. Nat Cell Biol 2: 871-878
8. Janicki SM, Tsukamoto T, Salghetti S.E., Tansey WP, Sachidanandam R, Prasanth KV, Ried T, Shav-Tal Y, Bertrand E., Singer RH, Spector D.L. (2004) From silencing to gene expression: realtime analysis in single cells. Cell 116: 683-698
9. Rafalska-Metcalf I.U., Powers SL, Joo L.M., LeRoy G., Janicki S.M. (2010) Single cell analysis of transcriptional activation dynamics. PLoS One 5: e10272
10. Masui O, Bonnet I, Le Baccon P, Brito I, Pollex T., Murphy N, Hupe P, Barillot E., Belmont AS, Heard E. (2011) Live-cell chromosome dynamics and outcome of X chromosome pairing events during ES cell differentiation. Cell 145: 447-458
11. Shav-Tal Y., Darzacq X, Shenoy S.M., Fusco D., Janicki SM, Spector DL, Singer R.H. (2004) Dynamics of single mRNPs in nuclei of living cells. Science 304: 1797-1800