Nuclear localization of P-glycoprotein is responsible for protection of the nucleus from doxorubicin in the resistant LoVo cell line

Biomedicine and Pharmacotherapy

Volumn 67, Issue 6, 2013, Pages 497-502

  Szaflarski, Witold ^a   Sujka Kordowska, Patrycja ^a   Januchowski, Radosław ^a   Wojtowicz, Karolina ^a   Andrzejewska, Małgorzata ^a   Nowicki, Michał ^a   Zabel, Maciej ^a,b  

^a POZNAN UNIVERSITY OF MEDICAL SCIENCES   (Poland)

^b WROCLAW MEDICAL UNIVERSITY   (Poland)

Author keywords

Cancer; Nuclear localization; P glycoprotein

Indexed keywords

DOXORUBICIN; MULTIDRUG RESISTANCE PROTEIN;

AGAR GEL ELECTROPHORESIS; ARTICLE; CANCER STEM CELL; CELL ISOLATION; CELL MEMBRANE; CELL NUCLEUS MEMBRANE; CONTROLLED STUDY; CYTOPLASM; DNA CLEAVAGE; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IN VITRO STUDY; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SURVIVAL RATE; WESTERN BLOTTING;

CANCER; NUCLEAR LOCALIZATION; P-GLYCOPROTEIN;

CELL LINE, TUMOR; CELL NUCLEUS; CYTOPLASM; DOXORUBICIN; DRUG RESISTANCE, MULTIPLE; HUMANS; NUCLEAR ENVELOPE; P-GLYCOPROTEIN; PROTEIN TRANSPORT;

EID: 84880931788     PISSN: 07533322     EISSN: 19506007     Source Type: Journal    
DOI: 10.1016/j.biopha.2013.03.011     Document Type: Article

References (20)

1. Urry D.W., Urry K.D., Szaflarski W., Nowicki M., Zabel M. Function and frustration of multi-drug ABC exporter protein and design of model proteins for drug delivery using protein hydration thermodynamics. Curr Pharm Des 2009, 15:2833-2867.
2. Hsu S.I., Lothstein L., Horwitz S.B. Differential overexpression of three mdr gene family members in multidrug-resistant J774.2 mouse cells. Evidence that distinct P-glycoprotein precursors are encoded by unique mdr genes. J Biol Chem 1989, 264:12053-12062.
3. Ueda K., Cornwell M.M., Gottesman M.M., Pastan I., Roninson I.B., Ling V., et al. The mdr1 gene, responsible for multidrug-resistance, codes for P-glycoprotein. Biochem Biophys Res Commun 1986, 141:956-962.
4. Wojtowicz K., Szaflarski W., Januchowski R., Zawierucha P., Nowicki M., Zabel M. Inhibitors of N-glycosylation as a potential tool for analysis of the mechanism of action and cellular localisation of glycoprotein P. Acta Biochim Pol 2012, 59:445-450.
5. Thiebaut F., Tsuruo T., Hamada H., Gottesman M.M., Pastan I., Willingham M.C. Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues. Proc Natl Acad Sci U S A 1987, 84:7735-7738.
6. Wang R.B., Kuo C.L., Lien L.L., Lien E.J. Structure-activity relationship: analyses of p-glycoprotein substrates and inhibitors. J Clin Pharm Ther 2003, 28:203-228.
7. Urry D.W., Urry K.D., Szaflarski W., Nowicki M. Elastic-contractile model proteins: physical chemistry, protein function and drug design and delivery. Adv Drug Deliv Rev 2010, 62:1404-14055.
8. Juliano R. Drug-resistant mutants of Chinese hamster ovary cells possess an altered cell surface carbohydrate component. J Supramol Struct 1976, 4:521-526.
9. Germann U.A. P-glycoprotein-a mediator of multidrug resistance in tumour cells. Eur J Cancer 1996, 32A:927-944.
10. Breier A., Barancik M., Sulova Z., Uhrik B. P-glycoprotein-implications of metabolism of neoplastic cells and cancer therapy. Curr Cancer Drug Targets 2005, 5:457-468.
11. Gervasoni J.E., Fields S.Z., Krishna S., Baker M.A., Rosado M., Thuraisamy K., et al. Subcellular distribution of daunorubicin in P-glycoprotein-positive and -negative drug-resistant cell lines using laser-assisted confocal microscopy. Cancer Res 1991, 51:4955-4963.
12. Molinari A., Cianfriglia M., Meschini S., Calcabrini A., Arancia G. P-glycoprotein expression in the Golgi apparatus of multidrug-resistant cells. Int J Cancer 1994, 59:789-795.
13. Willingham M.C., Richert N.D., Cornwell M.M., Tsuruo T., Hamada H., Gottesman M.M., et al. Immunocytochemical localization of P170 at the plasma membrane of multidrug-resistant human cells. J Histochem Cytochem 1987, 35:1451-1456.
14. Babakhanian K., Bendayan M., Bendayan R. Localization of P-glycoprotein at the nuclear envelope of rat brain cells. Biochem Biophys Res Commun 2007, 361:301-306.
15. Calcabrini A., Meschini S., Stringaro A., Cianfriglia M., Arancia G., Molinari A. Detection of P-glycoprotein in the nuclear envelope of multidrug resistant cells. Histochem J 2000, 32:599-606.
16. Gabbay E.J., Grier D., Fingerle R.E., Reimer R., Levy R., Pearce S.W., et al. Interaction specificity of the anthracyclines with deoxyribonucleic acid. Biochemistry 1976, 15:2062-2070.
17. Szaflarski W., Sujka-Kordowska P., Januchowski R., Jaszczyńska-Nowinka K., Andrzejewska M., Savan A., et al. Expression of cell vault components MVP, TEP1 and vPARP in cancerous ovarian tissues. Adv Clin Exp Med 2011, 20:285-294.
18. Kasprzak A., Szaflarski W., Szmeja J., Andrzejewska M., Przybyszewska W., Kaczmarek E., et al. Differential expression of IGF-1 mRNA isoforms in colorectal carcinoma and normal colon tissue. Int J Oncol 2013, 42:305-316.
19. Poland J., Schadendorf D., Lage H., Schnolzer M., Celis J.E., Sinha P. Study of therapy resistance in cancer cells with functional proteome analysis. Clin Chem Lab Med 2002, 40:221-234.
20. Fu D., Arias I.M. Intracellular trafficking of P-glycoprotein. Int J Biochem Cell Biol 2012, 44:461-464.