In vitro cytotoxicity assessment of ulvan, a polysaccharide extracted from green algae

Phytotherapy Research

Volumn 27, Issue 8, 2013, Pages 1143-1148

  Alves, Anabela ^a   Sousa, Rui A ^a   Reis, Rui L ^a  

^a UNIVERSITY OF MINHO   (Portugal)

Author keywords

cytotoxicity; DNA quantification; green algae; MTS; protein; Ulvan

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; ALGAL EXTRACT; DOUBLE STRANDED DNA; HYALURONIC ACID; ULVAN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL ASSAY; CELL COUNT; CELL METABOLISM; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG CYTOTOXICITY; FIBROBLAST; IN VITRO STUDY; INCUBATION TIME; MOUSE; NONHUMAN; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; ULVA;

CHLOROPHYTA; ULVA LACTUCA;

CYTOTOXICITY; DNA QUANTIFICATION; GREEN ALGAE; MTS; PROTEIN; ULVAN;

ANIMALS; CELL LINE; CELL SURVIVAL; CYTOTOXINS; FIBROBLASTS; HYALURONIC ACID; MICE; POLYSACCHARIDES; TETRAZOLIUM SALTS; THIAZOLES; ULVA;

EID: 84880723916     PISSN: 0951418X     EISSN: 10991573     Source Type: Journal    
DOI: 10.1002/ptr.4843     Document Type: Article

References (50)

1. Alves A, Caridade SG, Mano JF, Sousa RA, Reis RL,. 2010. Extraction and physico-chemical characterization of a versatile biodegradable polysaccharide obtained from green algae. Carbohydr Res 345 (15): 2194-2200.
2. d' Ayala G, Malinconico M, Laurienzo P,. 2008. Marine Derived Polysaccharides for Biomedical Applications: Chemical Modification Approaches. Molecules 13 (9): 2069-2106.
3. Barile FA, Dierickx PJ, Kristen U,. 1994. In vitro cytotoxicity testing for prediction of acute human toxicity. Cell Biol Toxicol 10 (3): 155-162.
4. Bobin-Dubigeon C, Lahaye M, Barry J-L,. 1997. Human Colonic Bacterial Degradability of Dietary Fibres from Sea-Lettuce (Ulva sp.). J Sci Food Agric 73 (2): 149-159.
5. Bocanegra A, Bastida S, Benedi J, Rodenas S, Sanchez-Muniz FJ,. 2009. Characteristics and Nutritional and Cardiovascular-Health Properties of Seaweeds. J Med Food 12 (2): 236-258.
6. Brading JWE, Georg-Plant MMT, Hardy DM,. 1954. The Polysaccharide from the Alga Ulva lactuca-Purification, Hydrolysis, and Methylation of the Polysaccharide. J Chem Soc Part I: 319-324.
7. Castro R, Zarra I, Lamas J,. 2004. Water-soluble seaweed extracts modulate the respiratory burst activity of turbot phagocytes. Aquaculture 229 (1-4): 67-78.
8. Castro R, Piazzon MC, Zarra I, Leiro J, Noya M, Lamas J,. 2006. Stimulation of turbot phagocytes by Ulva rigida C. Agardh polysaccharides. Aquaculture 254 (1-4): 9-20.
9. Costa C, Alves A, Pinto P, et al,. 2012. Characterization of ulvan extracts to assess the effect of different steps in the extraction procedure. Carbohydr Polym 88: 537-546.
10. Cruz DMG, Ivirico JLE, Gomes MM, et al,. 2008. Chitosan microparticles as injectable scaffolds for tissue engineering. J Tissue Eng Regen Med 2 (6): 378-380.
11. Dash M, Chiellini F, Ottenbrite RM, Chiellini E,. 2011. Chitosan-A versatile semi-synthetic polymer in biomedical applications. Prog Polym Sci 36 (8): 981-1014.
12. Ehlers EM, Behrens P, Wünsch L, Kühnel W, Russlies M,. 2001. Effects of hyaluronic acid on the morphology and proliferation of human chondrocytes in primary cell culture. Ann Anat 183 (1): 13-17.
13. Ertesvag H, Valla S,. 1998. Biosynthesis and applications of alginates. Polym Degrad Stab 59 (1-3): 85-91.
14. Harada N, Maeda M,. 1998. Chemical Structure of Antithrombin-active Rhamnan Sulfate from Monostrom nitidum. Biosci Biotechnol Biochem 62 (9): 1647-1652.
15. ISO document 10993. 5. Biological compatibility of medical devices. 1992. Test for cytotoxicity: In vitro methods.
16. Jensen A,. 1993. Present and future needs for algae and algal products. Hydrobiologia 260-261 (1): 15-23.
17. Kaeffer B, Benard C, Lahaye M, Blottiere HM, Cherbut C,. 1999. Biological properties of ulvan, a new source of green seaweed sulfated polysaccharides, on cultured normal and cancerous colonic epithelial tells. Planta Med 65 (6): 527-531.
18. Kirkpatrick CJ, Mittermayer C,. 1990. Theoretical and practical aspects of testing potential biomaterials in vitro. J Mater Sci Mater Med 1 (1): 9-13.
19. Kogan G, Šoltés L, Stern R, Gemeiner P,. 2007. Hyaluronic acid: a natural biopolymer with a broad range of biomedical and industrial applications. Biotechnol Lett 29 (1): 17-25.
20. Kutty JK, Cho E, Soo Lee J, Vyavahare NR, Webb K,. 2007. The effect of hyaluronic acid incorporation on fibroblast spreading and proliferation within PEG-diacrylate based semi-interpenetrating networks. Biomaterials 28 (33): 4928-4938.
21. Lahaye M, Axelos MAV,. 1993. Gelling properties of water-soluble polysaccharides from proliferating marine green seaweeds (Ulva spp.). Carbohydr Polym 22 (4): 261-265.
22. Lahaye M, Robic A,. 2007. Structure and Functional Properties of Ulvan, a Polysaccharide from Green Seaweeds. Biomacromolecules 8 (6): 1765-1774.
23. 13C-NMR spectroscopic characterisation of ulvans from Ulva (Ulvales, Chlorophyta). J Appl Phycol 11 (1): 1-7.
24. Mabeau S, Fleurence J,. 1993. Seaweed in food products: biochemical and nutritional aspects. Trends Food Sci Technol 4 (4): 103-107.
25. Mano JF, Silva GA, Azevedo HS, et al,. 2007. Natural origin biodegradable systems in tissue engineering and regenerative medicine: present status and some moving trends. J R Soc Interface 4 (17): 999-1030.
26. Mao W, Zang X, Li Y, Zhang H,. 2006. Sulfated polysaccharides from marine green algae Ulva conglobata and their anticoagulant activity. J Appl Phycol 18 (1): 9-14.
27. Marques AP, Cruz HR, Coutinho OP, Reis RL,. 2005. Effect of starch-based biomaterials on the in vitro proliferation and viability of osteoblast-like cells. J Mater Sci Mater Med 16 (9): 833-842.
28. Oliveira JM, Grech JMR, Leonor IB, Mano JF, Reis RL,. 2007. Calcium-phosphate derived from mineralized algae for bone tissue engineering applications. Mater Lett 61 (16): 3495-3499.
29. Paradossi G, Cavalieri F, Pizzoferrato L, Liquori AM,. 1999. A physico-chemical study on the polysaccharide ulvan from hot water extraction of the macroalga Ulva. Int J Biol Macromol 25 (4): 309-315.
30. Pengzhan Y, Ning L, Xiguang L, Gefei Z, Quanbin Z, Pengcheng L,. 2003a. Antihyperlipidemic effects of different molecular weight sulfated polysaccharides from Ulva pertusa (Chlorophyta). Pharmacol Res 48 (6): 543-549.
31. Pengzhan Y, Quanbin Z, Ning L, Zuhong X, Yanmei W, Zhi'en L,. 2003b. Polysaccharides from Ulva pertusa (Chlorophyta) and preliminary studies on their antihyperlipidemia activity. J Appl Phycol V15 (1): 21-27.
32. Percival E, Wold JK,. 1963. Acid Polysaccharide from Green Seaweed Ulva lactuca.2. Site of Ester Sulphate. J Chem Soc Part V: 5459-5468.
33. Pizzoferrato A, Ciapetti G, Stea S, et al,. 1994. Cell culture methods for testing Biocompatibility. Clin Mater 15 (3): 173-180.
34. Qi H, Zhang Q, Zhao T, et al,. 2005a. Antioxidant activity of different sulfate content derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta) in vitro. Int J Biol Macromol 37 (4): 195-199.
35. Qi H, Zhao T, Zhang Q, Li Z, Zhao Z, Xing R,. 2005b. Antioxidant activity of different molecular weight sulfated polysaccharides from Ulva pertusa Kjellm (Chlorophyta). J Appl Phycol 17 (6): 527-534.
36. Quemener B, Lahaye M, Bobin-Dubigeon C,. 1997. Sugar determination in ulvans by a chemical-enzymatic method coupled to high performance anion exchange chromatography. J Appl Phycol V9 (2): 179-188.
37. Ray B, Lahaye M,. 1995a. Cell-wall polysaccharides from the marine green alga Ulva "rigida" (Ulvales, Chlorophyta). Chemical structure of ulvan. Carbohydr Res 274: 313-318.
38. Ray B, Lahaye M,. 1995b. Cell-wall polysaccharides from the marine green alga Ulva rigida (Ulvales, Chlorophyta). Chemical structure of ulvan. Carbohydr Res 274: 313-318.
39. Ray B, Lahaye M,. 1995c. Cell-wall polysaccharides from the marine green alga Ulva rigida (Ulvales, Chlorophyta). Extraction and chemical composition. Carbohydr Res 274: 251-261.
40. Renn DW,. 1984. Agar and agarose: indispensable partners in biotechnology. Ind Eng Chem Prod Res Dev 23 (1): 17-21.
41. Rinaudo M,. 2006. Chitin and chitosan: Properties and applications. Prog Polym Sci 31 (7): 603-632.
42. Robic A, Bertrand D, Sassi JF, Lerat Y, Lahaye M,. 2009. Determination of the chemical composition of ulvan, a cell wall polysaccharide from Ulva spp. (Ulvales, Chlorophyta) by FT-IR and chemometrics. J Appl Phycol 21: 451-456.
43. Salgado AJ, Coutinho OP, Reis RL,. 2004. Novel Starch-Based Scaffolds for Bone Tissue Engineering: Cytotoxicity, Cell Culture, and Protein Expression. Tissue Eng 10 (3-4): 465-474.
44. Skjelbred B, Horsberg TE, Tollefsen KE, Andersen T, Edvardsen B,. 2011. Toxicity of the ichthyotoxic marine flagellate Pseudochattonella (Dictyochophyceae, Heterokonta) assessed by six bioassays. Harmful Algae 10 (2): 144-154.
45. Song E, Kim SY, Chun T, Byun H-J, Lee YM,. 2006. Collagen scaffolds derived from a marine source and their biocompatibility. Biomaterials 27 (15): 2951-2961.
46. Tonnesen HH, Karlsen J,. 2002. Alginate in Drug Delivery Systems. Drug Dev Ind Pharm 28 (6): 621-630.
47. Tuzlakoglu K, Alves CM, Mano JF, Reis RL,. 2004. Production and Characterization of Chitosan Fibers and 3-D Fiber Mesh Scaffolds for Tissue Engineering Applications. Macromol Biosci 4 (8): 811-819.
48. Wang L, Shelton RM, Cooper PR, Lawson M, Triffitt JT, Barralet JE,. 2003. Evaluation of sodium alginate for bone marrow cell tissue engineering. Biomaterials 24 (20): 3475-3481.
49. Warrand J,. 2006. Healthy polysaccharides-The next chapter in food products. Food Technol Biotech 44 (3): 355-370.
50. Wiegand C, Hipler U-C,. 2008. Methods for the measurement of cell and tissue compatibility including tissue regeneration processes. Krankenhhyg Interdiszip 3 (1): Doc12. Tecnologia