Measurement of phagocytic engulfment of apoptotic cells by macrophages using phrodo succinimidyl ester

Current Protocols in Immunology

Volumn , Issue SUPPL.100, 2013, Pages

  Aziz, Monowar ^a   Yang, Weng Lang ^a   Wang, Ping ^a  

^a DONALD AND BARBARA ZUCKER SCHOOL OF MEDICINE AT HOFSTRA NORTHWELL   (United States)

Author keywords

Apoptotic cells; Flow cytometry; Macrophage; Phagocytosis; PHrodo SE

Indexed keywords

ESTER; PHRODO SUCCINIMIDYL ESTER; UNCLASSIFIED DRUG; FLUORESCENT DYE; SUCCINIMIDE DERIVATIVE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL PHAGOCYTOSIS; CONTROLLED STUDY; COVALENT BOND; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; LYSOSOME; MACROPHAGE; MOUSE; NONHUMAN; PHAGOCYTE; PHAGOLYSOSOME; PHAGOSOME; PRIORITY JOURNAL; ANIMAL; BAGG ALBINO MOUSE; C57BL MOUSE; CHEMISTRY; IMMUNOLOGY; METHODOLOGY; PH; PHAGOCYTOSIS;

ANIMALS; APOPTOSIS; FLOW CYTOMETRY; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; MACROPHAGES; MICE; MICE, INBRED BALB C; MICE, INBRED C57BL; PHAGOCYTOSIS; SUCCINIMIDES;

MLCS; MLOWN;

EID: 84880713815     PISSN: 19343671     EISSN: 1934368X     Source Type: Journal    
DOI: 10.1002/0471142735.im1431s100     Document Type: Article

References (19)

1. Albert, M.L., Kim, J.I., and Birge, R.B. 2000. Alphavbeta5 integrin recruits the CrkII-Dock180- rac1 complex for phagocytosis of apoptotic cells. Nat. Cell Biol. 2:899-905.
2. Fadok, V.A., Voelker, D.R., Campbell, P.A., Cohen, J.J., Bratton, D.L., and Henson, P.M. 1992. Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. J. Immunol. 148:2207-2216.
3. Hanayama, R., Tanaka, M., Miwa, K., Shinohara, A., Iwamatsu, A., and Nagata, S. 2002. Identification of a factor that links apoptotic cells to phagocytes. Nature 417:182-187.
4. Hanayama, R., Miyasaka, K., Nakaya, M., and Nagata, S. 2006. MFG-E8-dependent clearance of apoptotic cells, and autoimmunity caused by its failure. Curr. Dir. Autoimmun. 9:162-172.
5. Kam, P.C. and Ferch, N.I. 2000. Apoptosis: Mechanisms and clinical implications. Anaesthesia 55:1081-1093.
6. Kerr, J.F., Wyllie, A.H., and Currie, A.R. 1972. Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics. Br. J. Cancer 26:239-257.
7. Kobayashi, N., Karisola, P., Pẽna-Cruz, V., Dorfman, D.M., Jinushi, M., Umetsu, S.E., Butte, M.J., Nagumo, H.,Chernova, I.,Zhu, B., Sharpe, A.H., Ito, S., Dranoff, G., Kaplan, G.G., Casasnovas, J.M., Umetsu, D.T., Dekruyff, R.H., and Freeman, G.J. 2007. TIM-1 and TIM-4 glycoproteins bind phosphatidylserine and mediate uptake of apoptotic cells. Immunity 27:927-940.
8. Lauber, K.,Blumenthal, S.G.,Waibel, M., andWesselborg, S. 2004. Clearance of apoptotic cells: getting rid of the corpses. Mol. Cell 14:277-287.
9. Lyons, A.B. 2000. Analyzing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution. J. Immunol. Methods 243:147-154.
10. Maurin, M., Benoliel, A.M., Bongrand, P., and Raoult, D. 1992. Phagolysosomes of Coxiella burnetii-infected cell lines maintain an acidic pH during persistent infection. Infect. Immun. 60:5013-5016.
11. Miksa, M., Komura, H., Wu, R., Shah, K.G., and Wang, P. 2009. A novel method to determine the engulfment of apoptotic cells by macrophages using pHrodo succinimidyl ester. J. Immunol. Methods 342:71-77.
12. Nagata, S. 2010. Apoptosis and autoimmune diseases. Ann. N.Y. Acad. Sci. 1209:10-16.
13. Park,D., Tosello-Trampont,A.C., Elliott,M.R., Lu, M., Haney, L.B., Ma, Z., Klibanov, A.L., Mandell, J.W., Ravichandran, K.S. 2007. BAI1 is an engulfment receptor for apoptotic cells upstream of the ELMO/Dock180/Rac module. Nature 450:430-434.
14. Park, S.Y., Jung, M.Y., Kim, H.J., Lee, S.J., Kim, S.Y., Lee, B.H., Kwon, T.H., Park, R.W., and Kim, I.S. 2008. Rapid cell corpse clearance by stabilin-2, a membrane phosphatidylserine receptor. Cell Death Differ. 15:192-201.
15. Ravichandran, K.S. and Lorenz, U. 2007. Engulfment of apoptotic cells: Signals for a good meal. Nat. Rev. Immunol. 7:964-974.
16. Savill, J., Dransfield, I., Hogg, N., and Haslett, C. 1990. Vitronectin receptor-mediated phagocytosis of cells undergoing apoptosis. Nature 343:170-173.
17. Scott, R.S.,McMahon, E.J., Pop, S.M., Reap, E.A., Caricchio, R., Cohen, P.L., Earp, H.S., and Matsushima, G.K. 2001. Phagocytosis and clearance of apoptotic cells is mediated by MER. Nature 411:207-211.
18. Savill, J., Dransfield, I., Gregory, C., and Haslett, C. 2002. A blast from the past: Clearance of apoptotic cells regulates immune responses. Nat. Rev. Immunol. 2:965-975.
19. Scott, R.S.,McMahon, E.J., Pop, S.M., Reap, E.A., Caricchio, R., Cohen, P.L., Earp, H.S., and Matsushima, G.K. 2001. Phagocytosis and clearance of apoptotic cells is mediated by MER. Nature 411:207-211.