Isolation of embryonic ventricular endothelial cells

Journal of Visualized Experiments

Volumn , Issue 77, 2013, Pages

  Dyer, Laura A ^a   Patterson, Cam ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

Bioengineering; Biomedical engineering; Cardiology; Cell culture; Cell isolation; Cells; Cellular biology; Cultured; Embryo; Endothelial cells; Endothelium; Heart; Issue 77; Mammalian; Medicine; Molecular biology; Primary cells; Vascular

Indexed keywords

EID: 84880680690     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/50463     Document Type: Article

References (13)

1. Landecker, H. Culturing Life: How Cells Became Technologies. Presidents and Fellows of Harvard College, (2007).
2. Kan, C.Y., Wen, V.W., et al. Endothelial cell dysfunction and cytoskeletal changes associated with repression of p16(INK4a) during immortalization. Oncogene. doi:10.1038/onc.2011.645 (2012).
3. Dyer, L.A. & Patterson, C. Development of the endothelium: an emphasis on heterogeneity. Semin. Thromb. Hemost. 36, 227-235, doi:10.1055/s-0030-1253446 (2010).
4. Dong, Q.G., Bernasconi S., et al. A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants. Arteriosclerosis, Thrombosis, and Vascular Biology. 17, 1599-1604 (1997).
5. van Beijnum, J.R., Rousch, M., Castermans, K., van der Linden, E., & Griffioen, A.W. Isolation of endothelial cells from fresh tissues. Nat. Protoc. 3, 1085-1091 (2008).
6. Runyan, R.B. & Markwald, R.R. Invasion of mesenchyme into three-dimensional collagen gels: a regional and temporal analysis of interaction in embryonic heart tissue. Dev. Biol. 95, 108-114 (1983).
7. Arnaoutova, I. & Kleinman, H.K. In vitro angiogenesis: endothelial cell tube formation on gelled basement membrane extract. Nat. Protoc. 5, 628-635, doi:10.1038/nprot.2010.6 (2010).
8. Zeisberg, E.M., Potenta, S.E., Sugimoto, H., Zeisberg, M., & Kalluri, R. Fibroblasts in kidney fibrosis emerge via endothelial-to-mesenchymal transition. Journal of the American Society of Nephrology: JASN. 19, 2282-2287, doi:10.1681/ASN.2008050513 (2008).
9. Chang L., Noseda M., et al. Differentiation of vascular smooth muscle cells from local precursors during embryonic and adult arteriogenesis requires Notch signaling. PNAS. 109, 5993-6998, doi:10.1073/pnas.1118512109 (2012).
10. Fehrenbach, M.L., Cao, G., Williams, J.T., Finklestein, J.M., & DeLisser, H.M. Isolation of murine lung endothelial cells. American Journal of Physiology: Lung Cellular and Molecular Physiology. 296, L1095-L1103, doi:10.1152/;ajplung.90613.2008 (2009).
11. Frauli, M. & Ludwig, H. Inhibition of fibroblast proliferation in a culture of human endometrial stromal cells using a medium containing D-valine. Archives of Gynecology and Obstetrics. 241, 87-96 (1987).
12. Lazzaro, V.A., Walker, R.J., et al. Inhibition of fibroblast proliferation in L-valine reduced selective media. Research communications in chemical pathology and pharmacology. 75, 39-48 (1992).
13. Arima, S., Nishiyama, K., et al. Angiogenic morphogenesis driven by dynamic and heterogeneous collective endothelial cell movement. Development. 138, 4763-4776, doi:10.1242/dev.068023 (2011).