PCR testing can be as accurate as culture for diagnosis of Ichthyophonus hoferi in Yukon River Chinook salmon Oncorhynchus tshawytscha

Diseases of Aquatic Organisms

Volumn 105, Issue 1, 2013, Pages 21-25

  Hamazaki, Toshihide ^a   Kahler, Eryn ^a   Borba, Bonnie M ^a   Burton, Tamara ^a  

^a ALASKA DEPARTMENT OF FISH AND GAME   (United States)

Author keywords

Accuracy; Amplification; Diagnostic; Genetic; Parasite; Sensitivity; Specificity

Indexed keywords

ACCURACY ASSESSMENT; DISEASE PREVALENCE; HOST-PATHOGEN INTERACTION; PARASITE INFESTATION; POLYMERASE CHAIN REACTION; PROTIST; SALMONID CULTURE;

ANIMAL; ARTICLE; CANADA; FISH DISEASE; ICHTHYOSPOREA; ICHTHYOSPOREA INFECTION; ISOLATION AND PURIFICATION; PARASITOLOGY; RIVER; SALMON;

ANIMALS; CANADA; FISH DISEASES; MESOMYCETOZOEA; MESOMYCETOZOEA INFECTIONS; RIVERS; SALMON;

YUKON RIVER;

EID: 84880624361     PISSN: 01775103     EISSN: 16161580     Source Type: Journal    
DOI: 10.3354/dao02606     Document Type: Article

References (11)

1. Kahler E, Burton T, Hamazaki T, Borba BM, Jasper JR, Dehn LA (2007) Assessment of Ichthyophonus in Chinook salmon within the Yukon River drainage, 2004. Fishery Data Series No. 07-64. Alaska Department of Fish & Game, Anchorage, AK
2. Kahler E, Borba BM, Burton T, Dehn LA, Hamazaki T, Jasper JR (2011) Prevalence of Ichthyophonus in Chinook sal-mon entering the Yukon River and Tanana stock spawning grounds, 2004-2006. Fishery Data Series No. 11-11. Alaska Department of Fish & Game, Anchorage, AK
3. Kocan R (2009) Evidence that PCR diagnostics under-estimate infection prevalence of Ichthyophonus in Yukon River Chinook salmon. Available at www.rapidsresearch. com/2009-Kocan-PCR-as-diagnostic-tool-.pdf (accessed 1 July 2012)
4. Kocan RM, Hershberger PK, Winton J (2004) Ichthyophoniasis: an emerging disease of Chinook salmon in the Yukon River. J Aquat Anim Health 16: 58-72
5. Kocan R, Dolan H, Hershberger P (2011) Diagnostic methodology is critical for accurately determining the prevalence of Ichthyophonus infections in wild fish populations. J Parasitol 97: 344-348
6. Kramer-Schadt S, Holst JC, Skagen D (2010) Analysis of variables associated with the Ichthyophonus hoferi epizootics in Norwegian spring spawning herring, 1992-2009. Can J Fish Aquat Sci 67: 1862-1873
7. McNemar Q (1947) Note on the sampling error of the difference between correlated proportions or percentages. Psychometrika 12: 153-157
8. McVicar AH (1999) Ichthyophonus and related organisms. In: Woo PTK, Bruno DW (eds) Fish diseases and disorders, Vol 3: Viral, bacterial and fungal infections. CABI Publishing, New York, NY
9. Meyers TR (2009) Fish pathology section laboratory manual, 3rd edn. Special Publications No. 12. Alaska Department of Fish and Game, CF Division, Juneau, AK
10. Stanley PM (2003) The presence of nucleic acids does not demonstrate the presence of living microbes. Am J Infect Control 31: 515
11. Whipps CM, Burton T, Watral VG, St-Hilaire S, Kent ML (2006) Assessing the accuracy of a polymerase chain reaction test for Ichthyophonus hoferi in Yukon River Chinook salmon Oncorhynchus tshawytscha. Dis Aquat Org 68: 141-147