The presence of nucleic acids does not demonstrate the presence of living microbes [1]

American Journal of Infection Control

Volumn 31, Issue 8, 2003, Pages 515-

  Stanley, Patricia M ^a  

^a Minntech Corporation   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; RNA;

ANTISEPSIS; DISINFECTION; DNA DETERMINATION; LETTER; MICROBIAL IDENTIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS;

EID: 0348014395     PISSN: 01966553     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0196-6553(03)00080-4     Document Type: Letter

References (4)

1. Sheridan GEC, Masters CI, Shallcross J, Mackey B. Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells. Appl Environ Microbiol 1998;64:1313-18.
2. Deva AK, Vickery K, Zou J, West RH, Harris JP, Cossart YE. Establishment of an in-use testing method for evaluating disinfection of surgical instruments using the duck hepatitis B model. J Hosp Infect 1996;33:119-30.
3. Bachoon DS, Chen F, Hodson RE. RNA recovery and detection of mRNA by RT-PCR from preserved prokaryotic samples. FEMS Microbiol Lett 2001;201:127-32.
4. Birch L, Dawson CE, Cornett JH, Keer JT. A comparison of nucleic acid amplification techniques for the assessment of bacterial viability. Lett Appl Microbiol 2001;33:296-301.