Nephrin phosphorylation regulates podocyte adhesion through the PINCH-1-ILK-α-parvin complex

BMB Reports

Volumn 46, Issue 4, 2013, Pages 230-235

  Zha, Dongqing ^a   Chen, Cheng ^a   Liang, Wei ^a   Chen, Xinghua ^a   Ma, Tean ^a   Yang, Hongxia ^a   van Goor, Harry ^b   Ding, Guohua ^a  

^a RENMIN HOSPITAL OF WUHAN UNIVERSITY   (China)

^b UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

Author keywords

Cell adhesion; Nephrin phosphorylation; PINCH 1 ILK parvin complex; Podocyte

Indexed keywords

ACTIN; ACTIN BINDING PROTEIN; ANTINEOPLASTIC ANTIBIOTIC; INTEGRIN LINKED KINASE; INTEGRIN-LINKED KINASE; LIM PROTEIN; LIMS1 PROTEIN, MOUSE; MEMBRANE PROTEIN; NEPHRIN; PARVA PROTEIN, MOUSE; PROTEIN SERINE THREONINE KINASE; PUROMYCIN AMINONUCLEOSIDE; SIGNAL TRANSDUCING ADAPTOR PROTEIN; SMALL INTERFERING RNA;

ANIMAL; ARTICLE; CELL ADHESION; CELL LINE; CHEMICALLY INDUCED DISORDER; CYTOLOGY; CYTOSKELETON; DRUG ANTAGONISM; DRUG EFFECT; GENETICS; GLOMERULUS; MALE; METABOLISM; MOUSE; NEPHROSIS; PATHOLOGY; PHOSPHORYLATION; PODOCYTE; RAT; RNA INTERFERENCE; WISTAR RAT;

ACTINS; ADAPTOR PROTEINS, SIGNAL TRANSDUCING; ANIMALS; ANTIBIOTICS, ANTINEOPLASTIC; CELL ADHESION; CELL LINE; CYTOSKELETON; KIDNEY GLOMERULUS; LIM DOMAIN PROTEINS; MALE; MEMBRANE PROTEINS; MICE; MICROFILAMENT PROTEINS; NEPHROSIS; PHOSPHORYLATION; PODOCYTES; PROTEIN-SERINE-THREONINE KINASES; PUROMYCIN AMINONUCLEOSIDE; RATS; RATS, WISTAR; RNA INTERFERENCE; RNA, SMALL INTERFERING;

EID: 84880606332     PISSN: 19766696     EISSN: 1976670X     Source Type: Journal    
DOI: 10.5483/BMBRep.2013.46.4.270     Document Type: Article

References (21)

1. Faul, C., Asanuma, K., Yanagida-Asanuma, E., Kim, K. and Mundel, P. (2007) Actin up: regulation of podocyte structure and function by components of the actin cytoskeleton. Trends Cell Biol. 17, 428-437.
2. Schermer, B. and Benzing, T. (2009) Lipid-protein interactions along the slit diaphragm of podocytes. J. Am. Soc. Nephrol. 20, 473-478.
3. Miner, J. H. (2012) Life without nephrin: it's for the birds. J. Am. Soc. Nephrol. 23, 369-371.
4. Qin, X. S., Tsukaguchi, H., Shono, A., Yamamoto, A., Kurihara, H. and Doi, T. (2009) Phosphorylation of nephrin triggers its internalization by raft-mediated endocytosis. J. Am. Soc. Nephrol. 20, 2534-2545.
5. Reiser, J., Pixley, F. J., Hug, A., Kriz, W., Smoyer, W. E., Stanley, E. R. and Mundel, P. (2000) Regulation of mouse podocyte process dynamics by protein tyrosine phosphatases rapid communication. Kidney Int. 57, 2035-2042.
6. Lahdenperä, J., Kilpeläinen, P., Liu, X. L., Pikkarainen, T., Reponen, P., Ruotsalainen, V. and Tryggvason, K. (2003) Clustering-induced tyrosine phosphorylation of nephrin by Src family kinases. Kidney Int. 64, 404-413.
7. New, L. A., Keyvani Chahi, A. and Jones, N. (2013) Direct regulation of nephrin tyrosine phosphorylation by nck adaptor proteins. J. Biol. Chem. 288, 1500-1510.
8. Jim, B., Ghanta, M., Qipo, A., Fan, Y., Chuang, P. Y., Cohen, H. W., Abadi, M., Thomas, D. B. and He, J. C. (2012) Dysregulated nephrin in diabetic nephropathy of type 2 diabetes: a cross sectional study. PLoS One 7, e36041.
9. Wu, C. (2004) The PINCH-ILK-parvin complexes: assembly, functions and regulation. Biochim. Biophys. Acta. 1692, 55-62.
10. Wu, C. (2005) PINCH, N(i)ck and the ILK: network wiring at cell-matrix adhesions. Trends Cell Biol. 15, 460-466.
11. Braun, A., Bordoy, R., Stanchi, F., Moser, M., Kostka, G. G., Ehler, E., Brandau, O. and Fässler, R. (2003) PINCH2 is a new five LIM domain protein, homologous to PINCH and localized to focal adhesions. Exp. Cell Res. 284, 239-250.
12. Attwell, S., Mills, J., Troussard, A., Wu, C. and Dedhar, S. (2003) Integration of cell attachment, cytoskeletal localization, and signaling by integrin-linked kinase (ILK), CH-ILKBP, and the tumor suppressor PTEN. Mol. Biol. Cell 14, 4813-4825.
13. Legate, K. R., Montañez, E., Kudlacek, O. and Fässler, R. (2006) ILK, PINCH and parvin: the tIPP of integrin signalling. Nat. Rev. Mol. Cell Biol. 7, 20-31.
14. Dai, C., Stolz, D. B., Bastacky, S. I., St-Arnaud, R., Wu, C., Dedhar, S. and Liu, Y. (2006) Essential role of integrin-linked kinase in podocyte biology: Bridging the integrin and slit diaphragm signaling. J. Am. Soc. Nephrol. 17, 2164-2175.
15. Verma, R., Kovari, I., Soofi, A., Nihalani, D., Patrie, K. and Holzman, L. B. (2006) Nephrin ectodomain engagement results in src kinase activation, nephrin phosphorylation, nck recruitment, and actin polymerization. J. Clin. Invest. 116, 1346-1359.
16. Jones, N., Blasutig, I. M., Eremina, V., Ruston, J. M., Bladt, F., Li, H., Huang, H., Larose, L., Li, S. S., Takano, T., Quaggin, S. E. and Pawson, T. (2006) Nck adaptor proteins link nephrin to the actin cytoskeleton of kidney podocytes. Nature 440, 818-823.
17. Uchida, K., Suzuki, K., Iwamoto, M., Kawachi, H., Ohno, M., Horita, S. and Nitta, K. (2008) Decreased tyrosine phosphorylation of nephrin in rat and human nephrosis. Kidney Int. 73, 926-932.
18. Ren, Z., Liang, W., Chen, C., Yang, H., Singhal, P. C. and Ding, G. (2012) Angiotensin II induces nephrin dephosphorylation and podocyte injury: Role of caveolin-1. Cellular Signalling 24, 443-450.
19. Yang, Y., Guo, L., Blattner, S. M., Mundel, P., Kretzler, M. and Wu, C. (2005) Formation and phosphorylation of the PINCH1-integrin linked kinase-α-parvin complex are important for regulation of renal glomerular podocyte adhesion, architecture, and survival. J. Am. Soc. Nephrol. 16, 1966-1976.
20. Jung, K. Y., Chen, K., Kretzler, M. and Wu, C. (2007) TGF-b1 Regulates the PINCH-1-Integrin-Linked Kinase-α-Parvin Complex in Glomerular Cells. J. Am. Soc. Nephrol. 18, 66-73.
21. Humphries, M. J. (2009) Cell adhesion assays. Methods Mol. Biol. 522, 203-210.