Chloroplast-targeted expression of the codon-optimized truncated cry1Ah gene in transgenic tobacco confers a high level of protection against insects

Plant Cell Reports

Volumn 32, Issue 8, 2013, Pages 1299-1308

  Li, Xiuying ^a,b   Li, Shengyan ^b,c   Lang, Zhihong ^b   Zhang, Jie ^b   Zhu, Li ^b   Huang, Dafang ^a,b  

^a NORTHEAST AGRICULTURAL UNIVERSITY   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

^c CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Chloroplast targeting; Codon optimization; Insect resistance; Transgenic tobacco; Truncated cry1Ah gene

Indexed keywords

BACTERIAL PROTEIN; ENDOTOXIN; HEMOLYSIN; INSECTICIDAL CRYSTAL PROTEIN, BACILLUS THURINGIENSIS; MESSENGER RNA;

ALLELE; ANIMAL; ARTICLE; BIOLOGICAL PEST CONTROL; CHLOROPLAST; CODON; DNA BASE COMPOSITION; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; LARVA; LEPIDOPTERA; METABOLISM; PARASITOLOGY; PHYSIOLOGY; POLYMERASE CHAIN REACTION; TOBACCO; TRANSGENIC PLANT;

ALLELES; ANIMALS; BACTERIAL PROTEINS; BASE COMPOSITION; CHLOROPLASTS; CODON; ENDOTOXINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENETIC VECTORS; HEMOLYSIN PROTEINS; LARVA; LEPIDOPTERA; PEST CONTROL, BIOLOGICAL; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; RNA, MESSENGER; TOBACCO; TRANSFORMATION, GENETIC;

AGROBACTERIUM TUMEFACIENS; BACILLUS THURINGIENSIS; HELICOVERPA ARMIGERA; HEXAPODA; NICOTIANA TABACUM;

EID: 84880508006     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-013-1444-z     Document Type: Article

References (33)

1. Arencibia A, Vázquez RI, Prieto D, Téllez P, Carmona ER, Coego A, Hernández L, De la Riva GA, Selman-Housein G (1997) Transgenic sugarcane plants resistant to stem borer attack. Mol Breed 3: 247-255.
2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
3. Bravo A, Gill SS, Soberón M (2007) Mode of action of Bacillus thuringiensis cry and cyt toxins and their potential for insect control. Toxicon 49: 423-435.
4. Bruce BD (2000) Chloroplast transit peptides: structure, function and evolution. Trends Cell Biol 10: 440-447.
5. Campbell WH, Gowri G (1990) Codon usage in higher plants, green algae, and cyanobacteria. Plant Physiol 92: 1-11.
6. Fan PX, Wang XC, Kuang TY, Li YX (2009) An efficient method for the extraction of chloroplast proteins compatible for 2-DE and MS analysis. Electrophore 30: 3024-3033.
7. Gamborg OL, Murashige T, Thorpe TA, Vasil IK (1976) Plant tissue culture media. In Vitr Cell Dev Biol Plant 12: 473-478.
8. Gatehouse JA (2008) Biotechnological prospects for engineering insect-resistant plants. Plant Physiol 146: 881-887.
9. Hobbs SLA, Kpodar P, DeLong CMO (1990) The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants. Plant Mol Biol 15: 851-864.
10. Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227: 1229-1231.
11. Jabeen R, Khan MS, Zafar Y, Anjum T (2010) Codon optimization of cry1Ab gene for hyper expression in plant organelles. Mol Biol Rep 37: 1011-1017.
12. Jang I-C, Nahm BH, Kim J-K (1999) Subcellular targeting of green fluorescent protein to plastids in transgenic rice plants provides a high-level expression system. Mol Breed 5: 453-461.
13. Jang I-C, Lee K-H, Nahm BH, Kim J-K (2002) Chloroplast targeting signal of a rice rbcS gene enhances transgene expression. Mol Breed 9: 81-91.
14. Jones JDG, Gilbert DE, Grady KL, Jorgensen RA (1987) T-DNA structure and gene expression in petunia plants transformed by Agrobacterium tumefaciens C58 derivatives. Mol Gen Genet 207: 478-485.
15. Kim EH, Suh SC, Park BS, Shin KS, Kweon SJ, Han EJ, Park S-H, Kim YS, Kim J-K (2009) Chloroplast-targeted expression of synthetic cry1Ac in transgenic rice as an alternative strategy for increased pest protection. Planta 230: 397-405.
16. Kudla G, Lipinski L, Caffin F, Helwak A, Zylicz M (2006) High guanine and cytosine content increases mRNA levels in mammalian cells. PLoS Biol 4: 933-942.
17. Kumar S, Chandra A, Pandey KC (2008) Bacillus thuringiensis (Bt) transgenic crop: an environment friendly insect-pest management strategy. J Environ Biol 29: 641-653.
18. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15: 473-497.
19. Murray EE, Lotzer J, Eberle M (1989) Codon usage in plant genes. Nucleic Acids Res 17: 477-498.
20. Napoli C, Lemieux C, Jorgensen R (1990) Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2: 279-289.
21. Perlak FJ, Fuchs RL, Dean DA, McPherson SL, Fischhoff DA (1991) Modification of the coding sequence enhances plant expression of insect control protein genes. Proc Natl Acad Sci USA 88: 3324-3328.
22. Perlak FJ, Stone TB, Muskopf YM, Petersen LJ, Parker GB, McPherson SA, Wyman J, Love S, Reed G, Biever D, Fischhoff DA (1993) Genetically improved potatoes: protection from damage by Colorado potato beetles. Plant Mol Biol 22: 313-321.
23. Phee B-K, Cho J-H, Park S, Jung JH, Lee Y-H, Jeon J-S, Bhoo SH, Hahn T-R (2004) Proteomic analysis of the response of Arabidopsis chloroplast proteins to high light stress. Proteomics 4: 3560-3568.
24. Porebski S, Bailey LG, Baum BR (1997) Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep 15: 8-15.
25. Schmidt GW, Bartlett SG, Grossman AR, Cashmore AR, Chua N-H (1981) Biosynthetic pathways of two polypeptide subunits of the light-harvesting chlorophyll a/b protein complex. J Cell Biol 91: 468-478.
26. Schwember AR (2008) An update on genetically modified crops. Cienc Investig Agrar 35: 231-250.
27. Vaeck M, Reynaerts A, Höfte H, Jansens S, De Beuckeleer M, Dean C, Zabeau M, Montagu MV, Leemans J (1987) Transgenic plants protected from insect attack. Nature 328: 33-37.
28. Verma D, Daniell H (2007) Chloroplast vector systems for biotechnology applications. Plant Physiol 145: 1129-1143.
29. Vinogradov AE (2003) DNA helix: the importance of being GC-rich. Nucleic Acids Res 31: 1838-1844.
30. Weng LX, Deng HH, Xu JL, Li Q, Wang LH, Jiang ZD, Zhang HB, Li QW, Zhang LH (2006) Regeneration of sugarcane elite breeding lines and engineering of stem borer resistance. Pest Manag Sci 62: 178-187.
31. Weng LX, Deng HH, Xu JL, Li Q, Zhang YQ, Jiang ZD, Li QW, Chen JW, Zhang LH (2011) Transgenic sugarcane plants expressing high levels of modified cry1Ac provide effective control against stem borers in field trials. Transgenic Res 20: 759-772.
32. Wong EY, Hironaka CM, Fischhoff DA (1992) Arabidopsis thaliana small subunit leader and transit peptide enhance the expression of Bacillus thuringiensis proteins in transgenic plants. Plant Mol Biol 20: 81-93.
33. Xue J, Liang GM, Crickmore N, Li HT, He KL, Song FP, Feng X, Huang DF, Zhang J (2008) Cloning and characterization of a novel cry1A toxin from Bacillus thuringiensis with high toxicity to the Asian corn borer and other lepidopteran insects. FEMS Microbiol Lett 280: 95-101.