Chloroplast targeting signal of a rice rbcS gene enhances transgene expression

Molecular Breeding

Volumn 9, Issue 2, 2002, Pages 81-91

  Jang, In Cheol ^a   Lee, Kyung Hee ^a   Nahm, Baek Hie ^a   Kim, Ju Kon ^a  

^a Myong Ji University   (South Korea)

Author keywords

Green fluorescent protein; Mutant transit peptide; Plastid targeting; Transgene expression; Transgenic rice

Indexed keywords

CYTOLOGY; GENE ENCODING; GENE EXPRESSION; PEPTIDES; PLANTS (BOTANY);

EXPRESSION VECTORS; GREEN FLUORESCENT PROTEIN; MUTANT TRANSIT PEPTIDE; PEPTIDE SEQUENCES; PLASTID TARGETING; RBCS TRANSIT PEPTIDE; TRANSGENE EXPRESSION; TRANSGENEIC PLANTS; TRANSGENIC RICE; TRANSIT PEPTIDES;

FLUORESCENCE;

AGROBACTERIUM; EMBRYOPHYTA; EUKARYOTA;

EID: 0036345311     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026787308828     Document Type: Article

References (25)

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3. Engineered GFP as a vital reporter in plants
4. Messenger RNA targeting of rice seed storge proteins to specific ER subdomains
5. Transgenic expression of two marker genes under the control of an Arabidopsis rbcS promoter: Sequence encoding the rubisco transit peptide increase expression levels
6. Sequences downstream of translation start regulate quantitative expression of two petunia rbcS genes
7. Transgenic rice plants harboring an introduced potato proteinase inhibitor II gene are insect resistant
8. The sequence surrounding the translation initiation codon of the pea plasrocyanin gene increases translational efficiency of a reporter gene
9. Improved metabolic action of a bacterial lysine decarboxylase gene in tobacco hairy root cultures by its fusion to a rbcS transit peptide coding sequence
10. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries T-DNA
11. Subcellular targeting of green fluorescent protein to plastids in transgenic rice plants provides a high-level expression system
12. Targeting of proteins into chloroplasts
13. Molecular and genetic analysis of transgenic rice plants expressing the maize ribosome-inactivating protein b-32 gene and the herbicide resistance bar gene
14. Transit peptide mutations that impair in vitro and in vivo chloroplasts protein import do not affect accumulation of the α-subunit of chloroplast ATPase
15. The green fluorescent protein as a marker to visualize plant mitochondria in vivo
16. Vecvtors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers
17. Light-regulated and cell-specific expression of tomato rbcS-gusA and rice rbcS-gusA fusion gene in transgenic rice
18. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
19. Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation
20. Regions in the transit peptide of SSU essential for transport into chloroplasts
21. Molecular cloning
22. Domain structure of mitochondrial and chloroplast targeting peptides
23. Vicilin with carbosy-terminal KDEL is retained in the endoplasmic reticulum and accumulates to high levels in the leaves of transgenic plants
24. The importance of the transit peptide and the transported protein for protein import into chloroplasts
25. Arabidopsis thaliana small subunit leader and transit peptide enhance the expression of Bacillus thuringiensis proteins in transgenic plants