In vitro assays of orphan glycosyltransferases and their application to identify notch xylosyltransferases

Methods in Molecular Biology

Volumn 1022, Issue , 2013, Pages 307-320

  Sethi, Maya K ^a   Buettner, Falk F R ^a   Ashikov, Angel ^a   Bakker, Hans ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Epidermal growth factor (EGF) repeat; Glycosyltransferase assay; Hydrolysis; Notch; Nucleotide sugar; UDP xylose; Xylosyltransferase

Indexed keywords

GLYCOSYLTRANSFERASE; NUCLEOTIDE;

ARTICLE; ENZYME ACTIVITY; ENZYME SPECIFICITY; HYDROLYSIS; IN VITRO STUDY; PRIORITY JOURNAL;

EID: 84880493956     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-465-4_23     Document Type: Article

References (17)

1. Beyer TA, Sadler JE, Rearick JI, Paulson JC, Hill RL (1981) Glycosyltransferases and their use in assessing oligosaccharide structure and structure-function relationships. Adv Enzymol Relat Areas Mol Biol 52:23-175
2. Henrissat B, Surolia A, Stanley P (2009) A genomic view of glycobiology (chapter 7). In: Varki A, Cummings RD, Esko JD, Freeze HH, Stanley P, Bertozzi CR, Hart GW, Etzler ME (eds) Essentials of glycobiology, 2nd edn. Cold Spring Harbor, NY
3. Sethi MK, Buettner FF, Krylov VB, Takeuchi H, Nifantiev NE, Haltiwanger RS, Gerardy-Schahn R, Bakker H (2010) Identifi cation of glycosyltransferase 8 family members as xylosyltransferases acting on O-glucosylated notch epidermal growth factor repeats. J Biol Chem 285:1582-1586
4. Sethi MK, Buettner FF, Ashikov A, Krylov VB, Takeuchi H, Nifantiev NE, Haltiwanger RS, Gerardy-Schahn R, Bakker H (2012) Molecular cloning of a xylosyltransferase that transfers the second xylose to O-glucosylated epidermal growth factor repeats of notch. J Biol Chem 287:2739-2748
5. Larsen RD, Rajan VP, Ruff MM, Kukowska-Latallo J, Cummings RD, Lowe JB (1989) Isolation of a cDNA encoding a murine UDPgalactose: Β-D-galactosyl- 1,4-N-Acetyl-D-glucosaminide α-1,3-galactosyltransferase: Expression cloning by gene transfer. Proc Natl Acad Sci USA 86:8227-8231
6. Shinkai A, Shinoda K, Sasaki K, Morishita Y, Nishi T, Matsuda Y, Takahashi I, Anazawa H (1997) High-Level expression and purifi cation of a recombinant human α-1, 3-fucosyltransferase in baculovirus-infected insect cells. Protein Expr Purif 10:379-385
7. Prieels JP, Monnom D, Dolmans M, Beyer TA, Hill RL (1981) Co-purifi cation of the Lewis blood group N-Acetylglucosaminide α1 → 4 fucosyltransferase and an N-Acetylglucosaminide α1 → 3 fucosyltransferase from human milk. J Biol Chem 256:10456-10463
8. Palcic MM, Heerze LD, Pierce M, Hindsgaul O (1988) The use of hydrophobic synthetic glycosides as acceptors in glycosyltransferase assays. Glycoconj J 5:49-63
9. Shevchenko A, Wilm M, Vorm O, Mann M (1996) Mass spectrometric sequencing of proteins silver-Stained polyacrylamide gels. Anal Chem 68:850-858
10. Johswich A, Kraft B, Wuhrer M, Berger M, Deelder AM, Hokke CH, Gerardy-Schahn R, Bakker H (2009) Golgi targeting of Drosophila melanogaster β4GalNAcTB requires a DHHC protein family-related protein as a pilot. J Cell Biol 184:173-183
11. Kraft B, Johswich A, Kauczor G, Scharenberg M, Gerardy-Schahn R, Bakker H (2011) "Add-on" domains of Drosophila β1,4- Nacetylgalactosaminyltransferase B in the stem region and its pilot protein. Cell Mol Life Sci 68:4091-4100
12. Schwientek T, Keck B, Levery SB, Jensen MA, Pedersen JW, Wandall HH, Stroud M, Cohen SM, Amado M, Clausen H (2002) The Drosophila gene brainiac encodes a glycosyltransferase putatively involved in glycosphingolipid synthesis. J Biol Chem 277: 32421-32429
13. Palcic MM (1994) Glycosyltransferases in glycobiology. Methods Enzymol 230:300-316
14. Haines N, Irvine KD (2005) Functional analysis of Drosophila β1,4-N-Acetylgalactosaminyltransferases. Glycobiology 15:335-346
15. Moller S, Croning MD, Apweiler R (2001) Evaluation of methods for the prediction of membrane spanning regions. Bioinformatics 17:646-653
16. Senn HJ, Wagner M, Decker K (1983) Ganglioside biosynthesis in rat liver. Characterization of UDPgalactoseglucosylceramide galactosyltransferase and UDPgalactose-GM2 galactosyltransferase. Eur J Biochem 135:231-236
17. Krylov V, Ustyuzhanina N, Grachev A, Bakker H, Nifantiev N (2007) Stereoselective synthesis of the 3-Aminopropyl glycosides of α-D-Xyl-(1 → 3)β-D-Glc and α-D-Xyl-(1 → 3)-α-D-Xyl-(1 → 3)-β-D-Glc and of their corresponding N-octanoyl derivatives. Synthesis 2007:3147-3154