Synthetic phytochelatins complement a phytochelatin-deficient Arabidopsis mutant and enhance the accumulation of heavy metal(loid)s

Biochemical and Biophysical Research Communications

Volumn 434, Issue 3, 2013, Pages 664-669

  Shukla, Devesh ^a   Tiwari, Manish ^b   Tripathi, Rudra D ^b   Nath, Pravendra ^b   Trivedi, Prabodh Kumar ^b  

^a INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Italy)

^b CSIR NATIONAL BOTANICAL RESEARCH INSTITUTE   (India)

Author keywords

Arabidopsis; Cad1 3 mutant; Heavy metal(loid); Mutant complementation; Phytoremediation; Synthetic phytochelatin

Indexed keywords

HEAVY METAL; METALLOID; PHYTOCHELATIN; RNA; SYNTHETIC DNA;

ARABIDOPSIS; ARTICLE; CONTROLLED STUDY; GENE CONSTRUCT; GENE EXPRESSION; GENE SYNTHESIS; METAL BINDING; PHENOTYPE; PLANT GROWTH; PRIORITY JOURNAL; RNA EXTRACTION; ROOT LENGTH; GENETIC COMPLEMENTATION; GENETICS; METABOLISM; MOLECULAR CLONING; MUTATION; PLANT GENE; TRANSCRIPTION INITIATION;

ARABIDOPSIS;

ARABIDOPSIS; CLONING, MOLECULAR; GENES, PLANT; GENETIC COMPLEMENTATION TEST; METALS, HEAVY; MUTATION; PHYTOCHELATINS; TRANSCRIPTIONAL ACTIVATION;

MLCS; MLOWN;

EID: 84880486760     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2013.03.138     Document Type: Article

References (25)

1. E. Grill, S. Loffler, E.L. Winnacker, et al., Phytochelatins, the heavy metal-binding peptides of plants, are synthesized from glutathione by a specific γ-glutamylcysteine dipeptidyl transpeptidase (phytochelatin synthase), Proceedings of the National Academy of Sciences USA 86 (1989) 6838-6842.
2. C.S. Cobbett, P.B. Goldsbrough, Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis, Annual Review of Plant Biology 53 (2002) 159-182.
3. P.A. Rea, O.K. Vatamaniuk, D.J. Rigden, Weeds, worms, and more. Papain's long-lost cousin, phytochelatin synthase, Plant Physiology 136 (2004) 2463-2474.
4. S. Wojas, S. Clemens, J. Hennig, et al., Overexpression of phytochelatin synthase in tobacco: distinctive effects of AtPCS1 and CePCS genes on plant response to cadmium, Journal of Experimental Botany 92 (2008) 1-15.
5. D. Shukla, R. Kesari, S. Mishra, et al., Expression of phytochelatin synthase from aquatic macrophyte Ceratophyllum demersum L. enhances cadmium and arsenic accumulation in tobacco, Plant Cell Reports 31 (2012) 1687-1699.
6. S. Lee, J.S. Moon, T.S. Ko, et al., Overexpression of Arabidopsis phytochelatin synthase paradoxically leads to hypersensitivity to cadmium stress, Plant Physiology 131 (2003) 656-663.
7. M. Pomponi, V. Censi, V. Di Girolamo, et al., Overexpression of Arabidopsis phytochelatin synthase in tobacco plants enhances Cd2+ tolerance and accumulation but not translocation to the shoot, Planta 223 (2006) 180-190.
8. W. Bae, R.K. Mehra, Metal-binding characteristics of a phytochelatin analog (Glu-Cys)2Gly, Journal of Inorganic Biochemisry 68 (1997) 201-210.
9. C.T. Dameron, D.R. Winge, Characterization of peptide-coated cadmium-sulfide crystallites, Inorganic Chemisry 29 (1990) 1343-1348.
10. H. Satofuka, T. Fukui, M. Takagi, et al., Metal-binding properties of phytochelatin-related peptides, Journal of Inorganic Biochemistry 86 (2001) 595-602.
11. W. Bae, W. Chen, A. Mulchandani, et al., Enhanced bioaccumulation of heavy metals by bacterial cells displaying synthetic phytochelatins, Biotechnology Bioengineering 70 (2000) 518-524.
12. W. Bae, R.K. Mehra, A. Mulchandani, et al., Genetic engineering of Escherichia coli for enhanced uptake and bioaccumulation of mercury, Applied and Environmental Microbiology 67 (2001) 5335-5338.
13. W. Bae, A. Mulchandani, W. Chen, Cell surface display of synthetic phytochelatins using ice nucleation protein for enhanced heavy metal bioaccumulation, Journal of Inorganic Biochemistry 88 (2002) 223-227.
14. I. Bontidean, J. Ahlqvist, A. Mulchandani, et al., Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection, Biosensors and Bioelectronics 18 (2003) 547-553.
15. F. Pinaud, D. King, H.P. Moore, et al., Bioactivation and cell targeting of semiconductor cdse/zns nanocrystals with phytochelatin-related peptides, Journal of American Chemical Society 126 (2004) 6115-6123.
16. U.L. Lao, A. Chen, M.R. Matsumoto, et al., Cadmium removal from contaminated soil by thermally responsive elastin (ELPEC20), Biopolymers Biotechnology and Boengineering 98 (2007) 349-355.
17. S.J. Clough, A.F. Bent, Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana, Plant Journal 16 (1998) 735-743.
18. M.E. Schmoger, M. Oven, E. Grill, Detoxification of arsenic by phytochelatins in plants, Plant Physiology 122 (2000) 793-801.
19. R.D. Tripathi, S. Srivastava, S. Mishra, et al., Arsenic hazards: strategies for tolerance and remediation by plants, Trends Biotechnology 25 (2007) 158-165.
20. W.Y. Song, J. Park, D.G. Mendoza-Cózatl, et al., Arsenic tolerance in Arabidopsis is mediated by two ABCC-type phytochelatin transporters, Proceedings of the National Academy of Sciences USA 107 (2010) 21187-21192.
21. Y. Li, O.M. Dhankher, L. Carreira, et al., Overexpression of phytochelatin synthase in Arabidopsis leads to enhanced arsenic tolerance and cadmium hypersensitivity, Plant Cell Physiology 45 (2004) 1787-1797.
22. I.J. Pickering, R.C. Prince, M.J. George, et al., Reduction and coordination of arsenic in Indian mustard, Plant Physiology 122 (2000) 1171-1177.
23. J.M. Gong, D.A. Lee, J.I. Schroeder, Long-distance root to-shoot transport of phytochelatins and cadmium in Arabidopsis, Proceedings of the National Academy of Sciences USA 100 (2003) 10118-10123.
24. A. Chen, E.A. Komives, J.I. Schroeder, An improved grafting technique for mature Arabidopsis plants demonstrates long-distance shoot-to-root transport of phytochelatins in Arabidopsis, Plant Physiology 141 (2006) 108-120.
25. D.G. Mendoza-Cózatl, E. Butko, F. Springer, et al., Identification of high levels of phytochelatins, glutathione and cadmium in the phloem sap of Brassica napus. A role for thiol-peptides in the long-distance transport of cadmium and the effect of cadmium on iron translocation, Plant Journal 54 (2008) 249-259.