Rotavirus shedding in symptomatic and asymptomatic children using reverse transcription-quantitative PCR

Journal of Medical Virology

Volumn 85, Issue 9, 2013, Pages 1661-1668

  Mukhopadhya, Indrani ^a   Sarkar, Rajiv ^a   Menon, Vipin Kumar ^a   Babji, Sudhir ^a   Paul, Anu ^a   Rajendran, Priya ^a   Sowmyanarayanan, Thuppal V ^a   Moses, Prabhakar D ^a   Iturriza Gomara, Miren ^b   Gray, James J ^b   Kang, Gagandeep ^a  

^a CHRISTIAN MEDICAL COLLEGE   (India)

^b PUBLIC HEALTH ENGLAND   (United Kingdom)

Author keywords

Children; Rotavirus; RT qPCR; Virus shedding

Indexed keywords

COMPLEMENTARY DNA;

ARTICLE; ASYMPTOMATIC INFECTION; CHILD; CHILDHOOD; CLINICAL ARTICLE; CLINICAL ASSESSMENT; CONTROLLED STUDY; DIARRHEA; DNA SYNTHESIS; FECES ANALYSIS; FEMALE; GENOTYPING TECHNIQUE; HUMAN; MALE; PLASMID; PRESCHOOL CHILD; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ROTAVIRUS INFECTION; VIRUS GENE; VIRUS SHEDDING;

CHILDREN; ROTAVIRUS; RT-QPCR; VIRUS SHEDDING;

ANTIGENS, VIRAL; ASYMPTOMATIC DISEASES; CAPSID PROTEINS; CHILD, PRESCHOOL; FECES; FEMALE; HUMANS; INFANT; MALE; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; ROTAVIRUS INFECTIONS; VIRAL LOAD; VIROLOGY; VIRUS SHEDDING;

ROTAVIRUS; ROTAVIRUS A;

EID: 84880332535     PISSN: 01466615     EISSN: 10969071     Source Type: Journal    
DOI: 10.1002/jmv.23641     Document Type: Article

References (36)

1. Allen SR, Jafri M, Donnelly B, McNeal M, Witte D, Bezerra J, Ward R, Tiao GM. 2007. Effect of rotavirus strain on the murine model of biliary atresia. J Virol 81:1671-1679.
2. Banerjee I, Ramani S, Primrose B, Moses P, Iturriza-Gomara M, Gray JJ, Jaffar S, Monica B, Muliyil JP, Brown DW, Estes MK, Kang G. 2006. Comparative study of the epidemiology of rotavirus in children from a community-based birth cohort and a hospital in South India. J Clin Microbiol 44:2468-2474.
3. Banerjee I, Gladstone BP, Le Fevre AM, Ramani S, Iturriza-Gomara M, Gray JJ, Brown DW, Estes MK, Muliyil JP, Jaffar S, Kang G. 2007. Neonatal infection with G10P[11] rotavirus did not confer protection against subsequent rotavirus infection in a community cohort in Vellore, South India. J Infect Dis 195:625-632.
4. Blutt SE, Kirkwood CD, Parreno V, Warfield KL, Ciarlet M, Estes MK, Bok K, Bishop RF, Conner ME. 2003. Rotavirus antigenaemia and viraemia: A common event? Lancet 362:1445-1449.
5. Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J. 1990. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28:495-503.
6. Burns JW, Krishnaney AA, Vo PT, Rouse RV, Anderson LJ, Greenberg HB. 1995. Analyses of homologous rotavirus infection in the mouse model. Virology 207:143-153.
7. Chiappini E, Azzari C, Moriondo M, Galli L, de Martino M. 2005. Viraemia is a common finding in immunocompetent children with rotavirus infection. J Med Virol 76:265-267.
8. Estes M. 1996. Rotaviruses and their replication. In: Knipe DM, Howley PM, editors. Fields virology. 3rd edition. Philadelphia: Lippincott-Raven Publishers, pp 1625-1655.
9. Eydelloth RS, Vonderfecht SL, Sheridan JF, Enders LD, Yolken RH. 1984. Kinetics of viral replication and local and systemic immune responses in experimental rotavirus infection. J Virol 50:947-950.
10. Fischer TK, Valentiner-Branth P, Steinsland H, Perch M, Santos G, Aaby P, Molbak K, Sommerfelt H. 2002. Protective immunity after natural rotavirus infection: A community cohort study of newborn children in Guinea-Bissau, west Africa. J Infect Dis 186:593-597.
11. Fischer TK, Ashley D, Kerin T, Reynolds-Hedmann E, Gentsch J, Widdowson MA, Westerman L, Puhr N, Turcios RM, Glass RI. 2005. Rotavirus antigenemia in patients with acute gastroenteritis. J Infect Dis 192:913-919.
12. Gentsch JR, Glass RI, Woods P, Gouvea V, Gorziglia M, Flores J, Das BK, Bhan MK. 1992. Identification of group A rotavirus gene 4 types by polymerase chain reaction. J Clin Microbiol 30:1365-1373.
13. Gijbels I, Prosdocimi I. 2010. Loess. Wiley Interdiscip Rev Comput Stat 2:590-599.
14. Gladstone BP, Muliyil JP, Jaffar S, Wheeler JG, Le Fevre A, Iturriza-Gomara M, Gray JJ, Bose A, Estes MK, Brown DW, Kang G. 2008. Infant morbidity in an Indian slum birth cohort. Arch Dis Child 93:479-484.
15. Gladstone BP, Ramani S, Mukhopadhya I, Muliyil J, Sarkar R, Rehman AM, Jaffar S, Gomara MI, Gray JJ, Brown DW, Desselberger U, Crawford SE, John J, Babji S, Estes MK, Kang G. 2011. Protective effect of natural rotavirus infection in an Indian birth cohort. N Engl J Med 365:337-346.
16. Gouvea V, Glass RI, Woods P, Taniguchi K, Clark HF, Forrester B, Fang ZY. 1990. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol 28:276-282.
17. Iturriza-Gomara M, Kang G, Gray J. 2004. Rotavirus genotyping: Keeping up with an evolving population of human rotaviruses. J Clin Virol 31:259-265.
18. Jayanthi V, Chacko A, Gani IK, Mathan VI. 1989. Intestinal transit in healthy southern Indian subjects and in patients with tropical sprue. Gut 30:35-38.
19. Kang G, Iturriza-Gomara M, Wheeler JG, Crystal P, Monica B, Ramani S, Primrose B, Moses PD, Gallimore CI, Brown DW, Gray J. 2004. Quantitation of group A rotavirus by real-time reverse-transcription-polymerase chain reaction: Correlation with clinical severity in children in South India. J Med Virol 73:118-122.
20. Kapikian AZ, Hoshino Y, Chanock RM. 2001. Rotaviruses. In: Knipe DM, Howley PM, editors. Fields virology. 4th edition. Philadelphia: Lippincott-Raven Publishers, pp 1787-1833.
21. Kraft LM. 1961. Responses of the mouse to the virus of epidemic diarrhea of infant mice. Neutralizing antibodies and carrier state. Proc Anim Care Panel 11:125-136.
22. Miotti PG, Eiden J, Yolken RH. 1985. Comparative efficiency of commercial immunoassays for the diagnosis of rotavirus gastroenteritis during the course of infection. J Clin Microbiol 22:693-698.
23. Nagayoshi S, Yamaguchi H, Ichikawa T, Miyazu M, Morishima T, Ozaki T, Isomura S, Suzuki S, Hoshino M. 1980. Changes of the rotavirus concentration in faeces during the course of acute gastroenteritis as determined by the immune adherence hemagglutination test. Eur J Pediatr 134:99-102.
24. Pang XL, Lee B, Boroumand N, Leblanc B, Preiksaitis JK, Yu Ip CC. 2004. Increased detection of rotavirus using a real time reverse transcription-polymerase chain reaction (RT-PCR) assay in stool specimens from children with diarrhea. J Med Virol 72:496-501.
25. Qiao H, Zhaori G, Jiang Z, Chen Y, Chen Y, Hou D. 1999. Detection of group C rotavirus antigen in bile duct and liver tissues of an infant with extrahepatic biliary atresia. Chin Med J (Engl) 112:93-95.
26. Richardson S, Grimwood K, Gorrell R, Palombo E, Barnes G, Bishop R. 1998. Extended excretion of rotavirus after severe diarrhoea in young children. Lancet 351:1844-1848.
27. Ruuska T, Vesikari T. 1990. Rotavirus disease in Finnish children: Use of numerical scores for clinical severity of diarrhoeal episodes. Scand J Infect Dis 22:259-267.
28. Schwarz BA, Bange R, Vahlenkamp TW, Johne R, Muller H. 2002. Detection and quantitation of group A rotaviruses by competitive and real-time reverse transcription-polymerase chain reaction. J Virol Methods 105:277-285.
29. Tate JE, Burton AH, Boschi-Pinto C, Steele AD, Duque J, Parashar UD. 2012. 2008 Estimate of worldwide rotavirus-associated mortality in children younger than 5 years before the introduction of universal rotavirus vaccination programmes: A systematic review and meta-analysis. Lancet Infect Dis 12:136-141.
30. Velazquez FR, Matson DO, Calva JJ, Guerrero L, Morrow AL, Carter-Campbell S, Glass RI, Estes MK, Pickering LK, Ruiz-Palacios GM. 1996. Rotavirus infections in infants as protection against subsequent infections. N Engl J Med 335:1022-1028.
31. Vesikari T, Sarkkinen HK, Maki M. 1981. Quantitative aspects of rotavirus excretion in childhood diarrhoea. Acta Paediatr Scand 70:717-721.
32. Ward RL, Bernstein DI, Young EC, Sherwood JR, Knowlton DR, Schiff GM. 1986. Human rotavirus studies in volunteers: Determination of infectious dose and serological response to infection. J Infect Dis 154:871-880.
33. Ward RL, McNeal MM, Sheridan JF. 1990. Development of an adult mouse model for studies on protection against rotavirus. J Virol 64:5070-5075.
34. Wilde J, Yolken R, Willoughby R, Eiden J. 1991. Improved detection of rotavirus shedding by polymerase chain reaction. Lancet 337:323-326.
35. Xu L, Harbour D, McCrae MA. 1990. The application of polymerase chain reaction to the detection of rotaviruses in faeces. J Virol Methods 27:29-37.
36. Yin JL, Shackel NA, Zekry A, McGuinness PH, Richards C, Putten KV, McCaughan GW, Eris JM, Bishop GA. 2001. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for measurement of cytokine and growth factor mRNA expression with fluorogenic probes or SYBR Green I. Immunol Cell Biol 79:213-221.