Amplification and detection methods

Molecular Diagnostics of Infectious Diseases

Volumn , Issue , 2010, Pages 53-66

  Bustin, Stephen ^a   Kessler, Harald ^b  

^a ROYAL LONDON HOSPITAL   (United Kingdom)

^b MEDICAL UNIVERSITY OF GRAZ   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84880184911     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1515/9783110214864     Document Type: Chapter

References (10)

1. Bustin, S.A., Nolan, T. (2004) Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J. Biomol. Technol. 15:155-166.
2. Bustin, S.A., Mueller, R. (2005) Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis. Clin. Sci. (Lond.) 109:365-379.
3. Bustin, S.A. (2008) Molecular medicine, gene-expression profiling and molecular diagnostics: putting the cart before the horse. Biomark. Med. 2:201-207.
4. Bustin, S.A. (2008) Real-time quantitative PCR-opportunities and pitfalls. Eur. Pharm. Rev. 4: 18-23.
5. Bustin, S.A. (2008) RT-qPCR and molecular diagnostics: no evidence for measles virus in the GI tract of autistic children. Eur. Pharm. Rev. Dig. 1:11-16.
6. Bustin, S.A., Benes, V., Garson, J.A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M.W., Shipley, G.L., Vandesompele, J., Wittwer, C.T. (2009) The MIQE Guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. 55:609-620.
7. Garson, J.A., Huggett, J.F., Bustin, S.A., Pfaffl, M.W., Benes, V.,Vandesompele, J., Shipley, G.L.(2009) Unreliable real-time PCR analysis of human endogenous retrovirus-W (HERV-W) RNA expression and DNA copy number in multiple sclerosis. AIDS Res. Hum. Retroviruses 25: 377-378.
8. Kessler, H.H. (2007) Design and work-up of a new molecular diagnostic assay based on real-time PCR. In: Protocols for Nucleic Acid Analysis by Nonradioactive Probes, 2nd edn, Hilario, E., Mackay, J. eds., pp. 227-236.
9. Lyon, E., Wittwer, C.T. (2009) LightCycler technology in molecular diagnostics. J. Mol. Diagn. 11:93-101.
10. Murphy, J., Bustin, S.A. (2009) Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard? Expert Rev. Mol. Diagn. 9:187-197.