Virus-induced gene silencing using artificial miRNAs in Nicotiana benthamiana

Methods in Molecular Biology

Volumn 975, Issue , 2013, Pages 99-107

  Tang, Yang ^a   Lai, Yizhen ^a   Liu, Yule ^a  

^a TSINGHUA UNIVERSITY   (China)

Author keywords

Artificial miRNA; Cabbage leaf curl virus; MIR VIGS; RNA silencing; Virus induced gene silencing

Indexed keywords

MICRORNA;

ARTICLE; BACTERIUM TRANSFORMATION; BEGOMOVIRUS; GENE SILENCING; GENE VECTOR; GENETICS; METHODOLOGY; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PLANT GENE; RHIZOBIUM RADIOBACTER; RNA INTERFERENCE; TOBACCO; VIROLOGY;

AGROBACTERIUM TUMEFACIENS; BASE SEQUENCE; BEGOMOVIRUS; CLONING, MOLECULAR; GENE KNOCKDOWN TECHNIQUES; GENES, PLANT; GENETIC VECTORS; MICRORNAS; RNA INTERFERENCE; TOBACCO; TRANSFORMATION, BACTERIAL;

MLCS; MLOWN;

EID: 84880172797     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-278-0_8     Document Type: Article

References (20)

1. Robertson D (2004) VIGS vectors for gene silencing: many targets, many tools. Annu Rev Plant Biol 55:495-519
2. Schwab R, Ossowski S, Riester M et al (2006) Highly specific gene silencing by artificial microRNAs in Arabidopsis. Plant Cell 18:1121-1133
3. Ossowski S, Schwab R, Weigel D (2008) Gene silencing in plants using artificial microRNAs and other small RNAs. Plant J 53:674-690
4. Tang Y, Wang F, Zhao J et al (2010) Virusbased microRNA expression for gene functional analysis in plants. Plant Physiol 153: 632-641
5. Schwab R, Palatnik JF, Riester M et al (2005) Specific effects of microRNAs on the plant transcriptome. Dev Cell 8:517-527
6. Hill JE, Strandberg JO, Hiebert E et al (1998) Asymmetric infectivity of pseudorecombinants of cabbage leaf curl virus and squash leaf curl virus: implications for bipartite geminivirus evolution and movement. Virology 250: 283-292
7. Paximadis M, Idris AM, Torres-Jerez I et al (1999) Characterization of tobacco geminiviruses in the Old and New World. Arch Virol 144:703-717
8. Turnage MA, Muangsan N, Peele CG et al (2002) Geminivirus-based vectors for gene silencing in Arabidopsis. Plant J 30:107-114
9. Muangsan N, Robertson D (2004) Geminivirus vectors for transient gene silencing in plants. Methods Mol Biol 265:101-115
10. Wise AA, Liu Z, Binns AN (2006) Three methods for the introduction of foreign DNA into Agrobacterium. Methods Mol Biol 343: 43-53
11. Chen C, Ridzon DA, Broomer AJ et al (2005) Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res 33:e179
12. Tang F, Hajkova P, Barton SC et al (2006) MicroRNA expression profiling of single whole embryonic stem cells. Nucleic Acids Res 34:e9
13. Varkonyi-Gasic E, Wu R, Wood M et al (2007) Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs. Plant Methods 3:12
14. Khraiwesh B, Fattash I, Arif MA et al (2011) Gene function analysis by artificial microRNAs in Physcomitrella patens. Methods Mol Biol 744:57-79
15. Manavella PA, Rubio-Somoza I (2011) Engineering elements for gene silencing: the artificial microRNAs technology. Methods Mol Biol 732:121-130
16. Schwab R, Ossowski S, Warthmann N et al (2010) Directed gene silencing with artificial microRNAs. Methods Mol Biol 592:71-88
17. Eamens AL, Waterhouse PM (2011) Vectors and methods for hairpin RNA and artificial microRNA-mediated gene silencing in plants. Methods Mol Biol 701:179-197
18. Varkonyi-Gasic E, Hellens RP (2010) qRTPCR of Small RNAs. Methods Mol Biol 631:109-122
19. Chen C, Tan R, Wong L et al (2011) Quantitation of microRNAs by real-time RT-qPCR. Methods Mol Biol 687:113-134
20. Varkonyi-Gasic E, Hellens RP (2011) Quantitative stem-loop RT-PCR for detection of microRNAs. Methods Mol Biol 744: 145-157