A cellular replicon-based phenotyping assay to determine susceptibility of hepatitis C virus clinical isolates to NS3/4A protease inhibitors

Methods in Molecular Biology

Volumn 1030, Issue , 2013, Pages 105-117

  Vijgen, Leen ^a   Verbeeck, Jannick ^b   Van Kerckhove, Barbara ^a   Berke, Jan Martin ^a   Koletzki, Diana ^a   Fanning, Gregory ^a   Lenz, Oliver ^a  

^a JANSSEN RESEARCH FOUNDATION   (Belgium)

^b UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

HCV; Hepatitis C virus; NS3 4A; Phenotyping assay; Protease inhibitor; Replicon; Transient assay

Indexed keywords

NONSTRUCTURAL PROTEIN 3; NONSTRUCTURAL PROTEIN 4A; PLASMID DNA; PROTEINASE INHIBITOR; VIRUS DNA;

AMINO ACID SEQUENCE; ANTIVIRAL SUSCEPTIBILITY; ARTICLE; DEPHOSPHORYLATION; DNA ISOLATION; DNA TRANSCRIPTION; GEL ELECTROPHORESIS; GENE SEQUENCE; HEPATITIS C VIRUS; HUMAN; HUMAN CELL; IN VITRO STUDY; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPLICON; SEQUENCE ANALYSIS;

ANTIVIRAL AGENTS; CELL LINE; CELL-FREE SYSTEM; CLONING, MOLECULAR; GENETIC VECTORS; HEPACIVIRUS; HUMANS; MICROBIAL SENSITIVITY TESTS; POLYMERASE CHAIN REACTION; PROTEASE INHIBITORS; TRANSCRIPTION, GENETIC; VIRAL NONSTRUCTURAL PROTEINS; VIRUS REPLICATION;

EID: 84880099805     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-484-5_9     Document Type: Article

References (3)

1. Pawlotsky JM (2011) The results of phase III clinical trials with telaprevir and boceprevir presented at the Liver Meeting 2010: a new standard of care for hepatitis C virus genotype 1 infection, but with issues still pending. Gastroenterology 140:746-754
2. Vijgen L, Lenz O, Berke JM et al (2010) A cellular replicon-based phenotyping assay to determine susceptibility of clinical isolates to NS3/4A protease inhibitors. Antivir Ther 15(Suppl 2): A116
3. Lohmann V, Körner F, Koch U et al (1999) Replication of subgenomic hepatitis C virus RNA in a hepatoma cell line. Science 285: 110-113