Quantification of microglial phagocytosis by a flow cytometer-based assay

Methods in Molecular Biology

Volumn 1041, Issue , 2013, Pages 121-127

  Pul, Refik ^a   Chittappen, Kandiyil Prajeeth ^a   Stangel, Martin ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Assay; Flow cytometry; Microglia; Phagocytosis; Polystyrene latex beads

Indexed keywords

LATEX; POLYSTYRENE;

ARTICLE; CELL ASSAY; CELL PHAGOCYTOSIS; FLOW CYTOMETRY; FLUORESCENCE; HISTOGRAM; MICROGLIA; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS;

ANIMALS; BIOLOGICAL ASSAY; FLOW CYTOMETRY; HUMANS; IMMUNOHISTOCHEMISTRY; MICROGLIA; PHAGOCYTOSIS;

EID: 84880088838     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-520-0_14     Document Type: Article

References (20)

1. Hamburger HJ (1912) Physikalisch-chemische untersuchungen über phagozyten. Ihre bedeutung von allgemein biologischem und pathologischem gesichtspunkt. In: Bergmann JF (ed). Wiesbaden
2. Fenn WO (1921) The phagocytosis of solid particles : I. Quartz. J Gen Physiol 3: 439-464
3. Harvath L, Terle DA (1999) Assay for phagocytosis. Methods Mol Biol 115:281-290
4. Crismon JM, Fuhrman FA (1947) Production of regional ischemia by intravascular injection of glass and plastic microspheres in graded sizes. Fed Proc 6:93
5. Sbarra AJ, Karnovsky ML (1959) The biochemical basis of phagocytosis. I. Metabolic changes during the ingestion of particles by polymorphonuclear leukocytes. J Biol Chem 234:1355-1362
6. Wilkins DJ (1964) Fluorescent Labelling of Polystyrene Latex for Tracing in Biological Systems. Nature 202:798-799
7. Dunn PA, Tyrer HW (1981) Quantitation of neutrophil phagocytosis, using fl uorescent latex beads. Correlation of microscopy and fl ow cytometry. J Lab Clin Med 98:374-381
8. Steinkamp JA, Wilson JS, Saunders GC et al (1982) Phagocytosis: fl ow cytometric quantitation with fl uorescent microspheres. Science 215:64-66
9. Schroeder F, Kinden DA (1983) Measurement of phagocytosis using fl uorescent latex beads. J Biochem Biophys Methods 8:15-27
10. Sanchez-Martin RM, Muzerelle M, Chitkul N et al (2005) Bead-based cellular analysis, sorting and multiplexing. Chembiochem 6: 1341-1345
11. von Zahn J, Moller T, Kettenmann H et al (1997) Microglial phagocytosis is modulated by pro-and anti-infl ammatory cytokines. Neuroreport 8:3851-3856
12. Pul R, Moharregh-Khiabani D, Skuljec J et al (2011) Glatiramer acetate modulates TNFalpha and IL-10 secretion in microglia and promotes their phagocytic activity. J Neuroimmune Pharmacol 6:381-388
13. Stangel M, Joly E, Scolding NJ et al (2000) Normal polyclonal immunoglobulins ('IVIg') inhibit microglial phagocytosis in vitro. J Neuroimmunol 106:137-144
14. Tambuyzer BR, Casteleyn C, Van Cruchten S et al (2012) Interferon-gamma modulates the functional profi le of in-vitro-cultured porcine microglia. Neuroreport 23:519-524
15. Dijkstra S, Geisert EE Jr, Dijkstra CD et al (2001) CD81 and microglial activation in vitro: proliferation, phagocytosis and nitric oxide production. J Neuroimmunol 114:151-159
16. Nagai A, Mishima S, Ishida Y et al (2005) Immortalized human microglial cell line: phenotypic expression. J Neurosci Res 81:342-348
17. Nagano T, Kimura SH, Takemura M (2010) Prostaglandin E2 reduces amyloid beta-induced phagocytosis in cultured rat microglia. Brain Res 1323:11-17
18. Lorenzi R, Brickell PM, Katz DR et al (2000) Wiskott-Aldrich syndrome protein is necessary for effi cient IgG-mediated phagocytosis. Blood 95:2943-2946
19. Aslam R, Kim M, Speck ER et al (2007) Platelet and red blood cell phagocytosis kinetics are differentially controlled by phosphatase activity within mononuclear cells. Transfusion 47:2161-2168
20. Keizer GD, Te Velde AA, Schwarting R et al (1987) Role of p150,95 in adhesion, migration, chemotaxis and phagocytosis of human monocytes. Eur J Immunol 17:1317-1322