Expression and Purification of Dengue Virus NS5 Polymerase and Development of a High-Throughput Enzymatic Assay for Screening Inhibitors of Dengue Polymerase

Methods in Molecular Biology

Volumn 1030, Issue , 2013, Pages 237-247

  Gong, Edwin Yunhao ^a   Kenens, Hannah ^a   Ivens, Tania ^b   Dockx, Koen ^a   Vermeiren, Katrien ^b   Vandercruyssen, Geneviève ^b   Devogelaere, Benoit ^b   Lory, Pedro ^a   Kraus, Guenter ^a  

^a JANSSEN RESEARCH FOUNDATION   (Belgium)

^b Biocartis SA   (Belgium)

Author keywords

Dengue virus; High throughput screening assay; NS5; Polymerase inhibitors; Protein purification; Protein expression; RNA dependent RNA polymerase; Scintillation proximity assay

Indexed keywords

BIOTIN; GUANOSINE TRIPHOSPHATE; METHYLTRANSFERASE; NONSTRUCTURAL PROTEIN 5; PLASMID VECTOR; POLYCYTIDYLIC ACID; RNA DIRECTED RNA POLYMERASE; STREPTAVIDIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIAL CELL; DENGUE VIRUS 2; ENZYMATIC ASSAY; HIGH THROUGHPUT SCREENING; LIQUID SCINTILLATION COUNTING; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RADIOACTIVITY; SCINTILLATION; VIRUS REPLICATION;

ANTIVIRAL AGENTS; DENGUE VIRUS; ENZYME ASSAYS; ENZYME INHIBITORS; ESCHERICHIA COLI; GENE EXPRESSION; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; PLASMIDS; RECOMBINANT PROTEINS; TRANSFORMATION, BACTERIAL; VIRAL NONSTRUCTURAL PROTEINS;

EID: 84880084672     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-484-5_19     Document Type: Article

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