In vitro suppression assay for functional assessment of human regulatory t cells

Methods in Molecular Biology

Volumn 979, Issue , 2013, Pages 199-212

  Tran, Dat Q ^a  

^a UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

Author keywords

Autoimmunity; FOXP3; Immunosuppression; Regulatory T cells; Suppression assay; Tregs

Indexed keywords

CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; CD3 ANTIBODY; CD4 ANTIGEN; HLA DR ANTIGEN; THYMIDINE; TRANSCRIPTION FACTOR FOXP3; 5 (6) CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; 5-(6)-CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; FLUORESCEIN DERIVATIVE; SUCCINIMIDE DERIVATIVE;

ANTIGEN PRESENTING CELL; ARTICLE; CD4+ CD25+ T LYMPHOCYTE; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNE CELL FUNCTION ASSAY; IMMUNOSUPPRESSIVE TREATMENT; IN VITRO STUDY; LYMPHOCYTE PROLIFERATION; PRIORITY JOURNAL; REGULATORY T LYMPHOCYTE; CELL PROLIFERATION; CYTOLOGY; FLOW CYTOMETRY; IMMUNOLOGY; LYMPHOCYTE ACTIVATION; METABOLISM; METHODOLOGY; STAINING;

ANTIGEN-PRESENTING CELLS; CELL PROLIFERATION; CYTOLOGICAL TECHNIQUES; FLOW CYTOMETRY; FLUORESCEINS; HLA-DR ANTIGENS; HUMANS; LYMPHOCYTE ACTIVATION; STAINING AND LABELING; SUCCINIMIDES; T-LYMPHOCYTES, REGULATORY; THYMIDINE;

MLCS; MLOWN;

EID: 84880071619     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-290-2_16     Document Type: Article

References (10)

1. Sakaguchi S, Miyara M, Costantino CM, Hafler DA (2010) FOXP3+ regulatory T cells in the human immune system. Nat Rev Immunol 10:490-500
2. Rudensky AY (2011) Regulatory T cells and Foxp3. Immunol Rev 241:260-8
3. van der Vliet HJ, Nieuwenhuis EE (2007) IPEX as a result of mutations in FOXP3. Clin Dev Immunol 2007:89017
4. Miyara M, Sakaguchi S (2011) Human FoxP3(+) CD4(+) regulatory T cells: their knowns and unknowns. Immunol Cell Biol 89:346-51
5. Cvetanovich GL, Ha fl er DA (2010) Human regulatory T cells in autoimmune diseases. Curr Opin Immunol 22:753-60
6. Sakaguchi S, Wing K, Onishi Y, Prieto-Martin P, Yamaguchi T (2009) Regulatory T cells: how do they suppress immune responses? Int Immunol 21:1105-11
7. Tran DQ, Glass DD, Uzel G, Darnell DA, Spalding C, Holland SM et al (2009) Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function. J Immunol 182:2929-38
8. Tang Q, Adams JY, Tooley AJ, Bi M, Fife BT, Serra P et al (2006) Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic mice. Nat Immunol 7:83-92
9. Onishi Y, Fehervari Z, Yamaguchi T, Sakaguchi S (2008) Foxp3+ natural regulatory T cells preferentially form aggregates on dendritic cells in vitro and actively inhibit their maturation. Proc Natl Acad Sci U S A 105: 10113-10118
10. Quah BJC, Parish CR (2010) The use of carboxy fl uorescein diacetate succinimidyl ester (CFSE) to monitor lymphocyte proliferation. J Vis Exp 44:e2259