Production of a novel silk-like protein from sea anemone and fabrication of wet-spun and electrospun marine-derived silk fibers

NPG Asia Materials

Volumn 5, Issue 6, 2013, Pages

  Yang, Yun Jung ^a   Choi, Yoo Seong ^b   Jung, Dooyup ^a   Park, Byung Rak ^a   Hwang, Woon Bong ^a   Kim, Hyun Woo ^c   Cha, Hyung Joon ^a  

^a Pohang University of Science and Technology (POSTECH)   (South Korea)

^b Chungnam National University   (South Korea)

^c Pukyong National University   (South Korea)

Author keywords

Electrospun nanofiber; Marine silk fibers; Recombinant silk proteins; Sea anemone; Wet spun fiber

Indexed keywords

BIO-INSPIRED MATERIALS; ELECTROSPUN NANOFIBERS; FIBROUS BIOMATERIALS; INFLAMMATORY REACTION; SEA ANEMONES; SILK FIBER; SILK PROTEINS; TECHNOLOGICAL APPLICATIONS;

BIOCOMPATIBILITY; BIODEGRADATION; BIOLOGICAL MATERIALS; FIBERS; MAMMALS; MECHANICAL PROPERTIES; RECOMBINANT PROTEINS; TEXTILE INDUSTRY;

SPINNING (FIBERS);

EID: 84879929711     PISSN: 18844049     EISSN: 18844057     Source Type: Journal    
DOI: 10.1038/am.2013.19     Document Type: Article

References (34)

1. Angel, M. V. Biodiversity of the pelagic ocean. Conserv. Biol. 7, 760-772 (1993).
2. Committee on Marine Biotechnology Marine Biotechnology in the Twenty-first Century: Problems, Promise, and Products 1-100 (The National Academies Press, Washington, 2002).
3. Greiner, A. & Wendorff, J. H. Electrospinning: a fascinating method for the preparation of ultrathin fibers. Angew. Chem. Int. Ed. 46, 5670-5703 (2007).
4. Kaplan, D. L., Adams, W. W., Farmer, B. & Viney, C. in Silk Polymers: Materials Science and Biotechnology (eds Kaplan, D. L., Adams, W. W., Farmer, B. & Viney, C.) 2-16 (American Chemical Society, Washington, 1994).
5. Altman, G. H., Diaz, F., Jakuba, C., Calabro, T., Horan, R. L., Chen, J., Lu, H., Richmond, J. & Kaplan, D. L. Silk-based biomaterials. Biomaterials 24, 401-416 (2003).
6. Shao, Z. & Vollrath, F. Surprising strength of silkworm silk. Nature 418, 741 (2002).
7. Heim, M., Keerl, D. & Scheibel, T. Spider silk: from soluble protein to extraordinary fiber. Angew. Chem. Int. Ed. 48, 3584-3596 (2009).
8. Vollrath, F. & Knight, D. P. Liquid crystalline spinning of spider silk. Nature 410, 541-548 (2001).
9. Omenetto, F. G. & Kaplan, D. L. New opportunities for an ancient material. Science 329, 528-531 (2010).
10. Ehrlich, H. Biological Materials of Marine Origin 3-21 (Springer, Berlin, 2010).
11. Darling, J. A., Reitzel, A. R., Burton, P. M., Ryan, J. F., Sullivan, J. C. & Finnerty, J. R. Rising starlet: the starlet sea anemone, Nematostella vectensis. Bioessays 27, 211-221 (2005).
12. Ozbek, S., Balasubramanian, P. G. & Holstein, T. W. Cnidocyst structure and the biomechanics of discharge. Toxicon 54, 1038-1045 (2009).
13. David, C. N., Ozbek, S., Adamczyk, P., Meier, S., Pauly, B., Chapman, J., Hwang, J. S., Gojobori, T. & Holstein, T. W. Evolution of complex structures: minicollagens shape the cnidarian nematocyst. Trends Genet. 24, 431-438 (2008).
14. Putnam, N. H., Srivastava, M., Hellsten, U., Dirks, B., Chapman, J., Salamov, A., Terry, A., Shapiro, H., Lindquist, E., Kapitonov, V. V., Jurka, J., Genikhovich, G., Grigoriev, I. V., Lucas, S. M., Steele, R. E., Finnerty, J. R., Technau, U., Martindale, M. Q. & Rokhsar, D. S. Sea anemone genome reveals ancestral eumetazoan gene repertoire and genomic organization. Science 317, 86-94 (2007).
15. Fournier, E. in Current Protocols in Food Analytical Chemistry (ed. Wrolstad, W. E). Ch. E, E1.1.1-E1.1.8 (John Wiley and Sons, Hoboken, 2001).
16. Izard, J. & Limberger, R. J. Rapid screening method for quantitation of bacterial cell lipids from whole cells. J. Microbiol. Methods 55, 411-418 (2003).
17. Holstein, T. W., Benoit, M., Herder, G. V., David, C. N., Wanner, G. & Gaub, H. E. Fibrous mini-collagens in hydra nematocysts. Science 265, 402-404 (1994).
18. Trevino, M., Stefanik, D. J., Rodriguez, R., Harmon, S. & Burton, P. M. Induction of canonical Wnt signaling by alsterpaullone is sufficient for oral tissue fate during regeneration and embryogenesis in Nematostella vectensis. Dev. Dyn. 240, 2673-2679 (2011).
19. Kluge, J. A., Rabotyagova, O., Leisk, G. G. & Kaplan, D. L. Spider silks and their applications. Trends Biotechnol. 26, 244-251 (2008).
20. Bandmann, N. & Nygren, P. A. Combinatorial expression vector engineering for tuning of recombinant protein production in Escherichia coli. Nucleic Acids Res 35, e32 (2007).
21. Goldberg, H. A. & Warner, K. J. The staining of acidic proteins on polyacrylamide gels: enhanced sensitivity and stability of 'Stains-all' staining in combination with silver nitrate. Anal. Biochem. 251, 227-233 (1997).
22. Xia, X. X., Qian, Z. G., Ki, C. S., Park, Y. H., Kaplan, D. L. & Lee, S. Y. Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber. Proc. Natl Acad. Sci. USA. 107, 14059-14063 (2010).
23. Northolt, M. G., den Decker, P., Picken, S. J., Baltussen, J. J. M. & Schlatmann, R. The tensile strength of polymer fibres. Adv. Polym. Sci. 178, 1-108 (2005).
24. Meyer, D. E. & Chilkoti, A. Purification of recombinant proteins by fusion with thermally-responsive polypeptides. Nat. Biotechnol. 17, 1112-1115 (1999).
25. Mello, C. M., Soares, J. W., Arcidiacono, S. & Butler, M. M. Acid extraction and purification of recombinant spider silk proteins. Biomacromolecules 5, 1849-1852 (2004).
26. Teulé, F., Cooper, A. R., Furin, W. A., Bittencourt, D., Rech, E. L., Brooks, A. & Lewis, R. V. A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning. Nat. Protoc. 4, 341-355 (2009).
27. Chen, X., Shao, Z. & Vollrath, F. The spinning processes for spider silk. Soft Matter 2, 448-451 (2006).
28. Brooks, A. E., Stricker, S. M., Joshi, S. B., Kamerzell, T. J., Middaugh, C. R. & Lewis, R. V. Properties of synthetic spider silk fibers based on Argiope aurantia MaSp2. Biomacromolecules 9, 1506-1510 (2008).
29. Teulé, F., Furin, W. A., Cooper, A. R., Duncan, J. R. & Lewis, R. V. Modifications of spider silk sequences in an attempt to control the mechanical properties of the synthetic fibers. J. Mater. Sci. 42, 8974-8985 (2007).
30. Pollock, C. M. & Shadwick, R. E. Relationship between body mass and biomechanical properties of limb tendons in adult mammals. Am. J. Physiol. 266, R1016-R1021 (1994).
31. Koombhongse, S., Liu, W. & Reneker, D. H. Flat polymer ribbons and other shapes by electrospinning. J. Polym. Sci. Pol. Phys. 39, 2598-2606 (2001).
32. Huang, L., McMillan, R. A., Apkarian, R. P., Pourdeyhimi, B., Conticello, V. P. & Chaikof, E. L. Generation of synthetic elastin-mimetic small diameter fibers and fiber networks. Macromolecules 33, 2989-2997 (2000).
33. Um, I. C., Kweon, H., Lee, K. G., Ihm, D. W., Lee, J.-H. & Park, Y. H. Wet spinning of silk polymer: I. Effect of coagulation conditions on the morphological feature of filament. Int. J. Biol. Macromol. 34, 89-105 (2004).
34. Wang, M., Jin, H. J., Kaplan, D. L. & Rutledge, G. C. Mechanical properties of electrospun silk fibers. Macromolecules 37, 6856-6864 (2004).