Extracellular location of Thermobifida fusca cutinase expressed in Escherichia coli BL21(DE3) without mediation of a signal peptide

Applied and Environmental Microbiology

Volumn 79, Issue 14, 2013, Pages 4192-4198

  Su, Lingqia ^a   Woodard, Ronald W ^b   Chen, Jian ^a   Wu, Jing ^a  

^a JIANGNAN UNIVERSITY   (China)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMICAL CHARACTERIZATION; ENZYMATIC PROPERTIES; ESCHERICHIA COLI BL21; HYDROLYTIC ACTIVITIES; IRREGULAR MORPHOLOGY; MEMBRANE PERMEABILITY; THERMOBIFIDA FUSCA; TRUNCATED VERSIONS;

CELL MEMBRANES; CYTOLOGY; ENZYMES; ESCHERICHIA COLI; INDUSTRIAL APPLICATIONS; PHOSPHOLIPIDS;

GENE ENCODING;

BACTERIAL PROTEIN; CARBOXYLESTERASE; CUTINASE; ESCHERICHIA COLI PROTEIN; RECOMBINANT PROTEIN; SIGNAL PEPTIDE;

BIOCHEMISTRY; ENZYME ACTIVITY; GENE EXPRESSION; HYDROLYSIS; MICROBIAL COMMUNITY; PEPTIDE; PHOSPHOLIPID;

ACTINOMYCETALES; ARTICLE; BIOSYNTHESIS; CULTURE MEDIUM; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; METABOLISM; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; SECRETION (PROCESS); SECRETORY PATHWAY; TRANSMISSION ELECTRON MICROSCOPY; WESTERN BLOTTING;

ACTINOMYCETALES; BACTERIAL PROTEINS; BLOTTING, WESTERN; CARBOXYLIC ESTER HYDROLASES; CLONING, MOLECULAR; CULTURE MEDIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC VECTORS; MICROSCOPY, ELECTRON, TRANSMISSION; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS; SECRETORY PATHWAY; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; THERMOBIFIDA FUSCA;

EID: 84879811582     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00239-13     Document Type: Article

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