High Throughput Gene Expression Analysis Identifies Reliable Expression Markers of Human Corneal Endothelial Cells

PLoS ONE

Volumn 8, Issue 7, 2013, Pages

  Chng, Zhenzhi ^a   Peh, Gary S L ^b   Herath, Wishva B ^c   Cheng, Terence Y D ^a   Ang, Heng Pei ^b   Toh, Kah Peng ^b   Robson, Paul ^c   Mehta, Jodhbir S ^b,d,e   Colman, Alan ^a  

^a INSTITUTE OF MEDICAL BIOLOGY   (Singapore)

^b SINGAPORE EYE RESEARCH INSTITUTE   (Singapore)

^c GENOME INSTITUTE OF SINGAPORE   (Singapore)

^d SINGAPORE NATIONAL EYE CENTRE   (Singapore)

^e DUKE NUS MEDICAL SCHOOL   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

RNA;

ADULT; AGED; ARTICLE; CELL ACTIVATION; CELL CULTURE; CELL ISOLATION; CELL TYPE; COL8A2 GENE; CORNEA CELL; CORNEA ENDOTHELIUM; CORNEA STROMA; CYYR1 GENE; ENDOTHELIUM CELL; FEMALE; FIBROBLAST; GENE; GENE EXPRESSION; GENE IDENTIFICATION; GENETIC ANALYSIS; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; IN VIVO STUDY; MALE; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; RELIABILITY; RNA SEQUENCE; SLC4A11 GENE; TISSUES;

ADULT; AGED; ANION TRANSPORT PROTEINS; ANTIPORTERS; AUTOPSY; BIOLOGICAL MARKERS; COLLAGEN TYPE VIII; CORNEAL KERATOCYTES; CORNEAL STROMA; ENDOTHELIAL CELLS; ENDOTHELIUM, CORNEAL; FEMALE; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMANS; MALE; MEMBRANE PROTEINS; MIDDLE AGED; ORGAN SPECIFICITY; PRIMARY CELL CULTURE;

EID: 84879778406     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0067546     Document Type: Article

References (49)

1. Peh GS, Beuerman RW, Colman A, Tan DT, Mehta JS, (2011) Human corneal endothelial cell expansion for corneal endothelium transplantation: an overview. Transplantation 91: 811-819.
2. Bourne WM, Nelson LR, Hodge DO, (1997) Central corneal endothelial cell changes over a ten-year period. Investigative ophthalmology & visual science 38: 779-782.
3. Joyce NC, (2003) Proliferative capacity of the corneal endothelium. Progress in retinal and eye research 22: 359-389.
4. Kaufman HE, Katz JI, (1977) Pathology of the corneal endothelium. Investigative ophthalmology & visual science 16: 265-268.
5. Edelhauser HF, (2000) The resiliency of the corneal endothelium to refractive and intraocular surgery. Cornea 19: 263-273.
6. Melles GR, Ong TS, Ververs B, van der Wees J, (2006) Descemet membrane endothelial keratoplasty (DMEK). Cornea 25: 987-990.
7. Rose L, Kelliher C, Jun AS, (2009) Endothelial keratoplasty: historical perspectives, current techniques, future directions. Canadian journal of ophthalmology Journal canadien d'ophtalmologie 44: 401-405.
8. Tan DT, Anshu A, Mehta JS, (2009) Paradigm shifts in corneal transplantation. Annals of the Academy of Medicine, Singapore 38: 332-338.
9. Barry PA, Petroll WM, Andrews PM, Cavanagh HD, Jester JV, (1995) The spatial organization of corneal endothelial cytoskeletal proteins and their relationship to the apical junctional complex. Investigative ophthalmology & visual science 36: 1115-1124.
10. Sugrue SP, Zieske JD, (1997) ZO1 in corneal epithelium: association to the zonula occludens and adherens junctions. Experimental eye research 64: 11-20.
11. Savion N, Farzame N, (1986) Characterization of the Na, K-ATPase pump in cultured bovine corneal endothelial cells. Experimental eye research 43: 355-363.
12. Corti C, Xuereb JH, Crepaldi L, Corsi M, Michielin F, et al. (2011) Altered levels of glutamatergic receptors and Na+/K+ ATPase-alpha1 in the prefrontal cortex of subjects with schizophrenia. Schizophrenia research 128: 7-14.
13. Sanchez C, Corrias A, Bueno-Orovio A, Davies M, Swinton J, et al. (2012) The Na+/K+ pump is an important modulator of refractoriness and rotor dynamics in human atrial tissue. American journal of physiology Heart and circulatory physiology 302: H1146-1159.
14. Sune G, Sarro E, Puigmule M, Lopez-Hellin J, Zufferey M, et al. (2010) Cyclophilin B interacts with sodium-potassium ATPase and is required for pump activity in proximal tubule cells of the kidney. PloS one 5: e13930.
15. Bonting SL, Simon KA, Hawkins NM, (1961) Studies on sodium-potassium-activated adenosine triphosphatase. I. Quantitative distribution in several tissues of the cat. Archives of biochemistry and biophysics 95: 416-423.
16. Wang Z, Gerstein M, Snyder M, (2009) RNA-Seq: a revolutionary tool for transcriptomics. Nature reviews Genetics 10: 57-63.
17. Huang da W, Sherman BT, Lempicki RA, (2009) Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nature protocols 4: 44-57.
18. Mi H, Dong Q, Muruganujan A, Gaudet P, Lewis S, et al. (2010) PANTHER version 7: improved phylogenetic trees, orthologs and collaboration with the Gene Ontology Consortium. Nucleic acids research 38: D204-210.
19. Tang F, Barbacioru C, Wang Y, Nordman E, Lee C, et al. (2009) mRNA-Seq whole-transcriptome analysis of a single cell. Nature methods 6: 377-382.
20. Sakai R, Kinouchi T, Kawamoto S, Dana MR, Hamamoto T, et al. (2002) Construction of human corneal endothelial cDNA library and identification of novel active genes. Investigative ophthalmology & visual science 43: 1749-1756.
21. Gottsch JD, Seitzman GD, Margulies EH, Bowers AL, Michels AJ, et al. (2003) Gene expression in donor corneal endothelium. Archives of ophthalmology 121: 252-258.
22. Bonanno JA, (2012) Molecular mechanisms underlying the corneal endothelial pump. Experimental eye research 95: 2-7.
23. Madan P, Rose K, Watson AJ, (2007) Na/K-ATPase beta1 subunit expression is required for blastocyst formation and normal assembly of trophectoderm tight junction-associated proteins. The Journal of biological chemistry 282: 12127-12134.
24. Violette MI, Madan P, Watson AJ, (2006) Na+/K+-ATPase regulates tight junction formation and function during mouse preimplantation development. Developmental biology 289: 406-419.
25. Sugi K, Musch MW, Field M, Chang EB, (2001) Inhibition of Na+,K+-ATPase by interferon gamma down-regulates intestinal epithelial transport and barrier function. Gastroenterology 120: 1393-1403.
26. Sonoda S, Sreekumar PG, Kase S, Spee C, Ryan SJ, et al. (2010) Attainment of polarity promotes growth factor secretion by retinal pigment epithelial cells: relevance to age-related macular degeneration. Aging 2: 28-42.
27. Desir J, Moya G, Reish O, Van Regemorter N, Deconinck H, et al. (2007) Borate transporter SLC4A11 mutations cause both Harboyan syndrome and non-syndromic corneal endothelial dystrophy. Journal of medical genetics 44: 322-326.
28. Jiao X, Sultana A, Garg P, Ramamurthy B, Vemuganti GK, et al. (2007) Autosomal recessive corneal endothelial dystrophy (CHED2) is associated with mutations in SLC4A11. Journal of medical genetics 44: 64-68.
29. Riazuddin SA, Vithana EN, Seet LF, Liu Y, Al-Saif A, et al. (2010) Missense mutations in the sodium borate cotransporter SLC4A11 cause late-onset Fuchs corneal dystrophy. Human mutation 31: 1261-1268.
30. Kapoor R, Bornstein P, Sage EH, (1986) Type VIII collagen from bovine Descemet's membrane: structural characterization of a triple-helical domain. Biochemistry 25: 3930-3937.
31. Biswas S, Munier FL, Yardley J, Hart-Holden N, Perveen R, et al. (2001) Missense mutations in COL8A2, the gene encoding the alpha2 chain of type VIII collagen, cause two forms of corneal endothelial dystrophy. Human molecular genetics 10: 2415-2423.
32. Gottsch JD, Sundin OH, Liu SH, Jun AS, Broman KW, et al. (2005) Inheritance of a novel COL8A2 mutation defines a distinct early-onset subtype of fuchs corneal dystrophy. Investigative ophthalmology & visual science 46: 1934-1939.
33. Gottsch JD, Zhang C, Sundin OH, Bell WR, Stark WJ, et al. (2005) Fuchs corneal dystrophy: aberrant collagen distribution in an L450W mutant of the COL8A2 gene. Investigative ophthalmology & visual science 46: 4504-4511.
34. Kobayashi A, Fujiki K, Murakami A, Kato T, Chen LZ, et al. (2004) Analysis of COL8A2 gene mutation in Japanese patients with Fuchs' endothelial dystrophy and posterior polymorphous dystrophy. Japanese journal of ophthalmology 48: 195-198.
35. Liskova P, Prescott Q, Bhattacharya SS, Tuft SJ, (2007) British family with early-onset Fuchs' endothelial corneal dystrophy associated with p.L450W mutation in the COL8A2 gene. The British journal of ophthalmology 91: 1717-1718.
36. Mok JW, Kim HS, Joo CK, (2009) Q455V mutation in COL8A2 is associated with Fuchs' corneal dystrophy in Korean patients. Eye 23: 895-903.
37. Yellore VS, Rayner SA, Emmert-Buck L, Tabin GC, Raber I, et al. (2005) No pathogenic mutations identified in the COL8A2 gene or four positional candidate genes in patients with posterior polymorphous corneal dystrophy. Investigative ophthalmology & visual science 46: 1599-1603.
38. Vitale L, Casadei R, Canaider S, Lenzi L, Strippoli P, et al. (2002) Cysteine and tyrosine-rich 1 (CYYR1), a novel unpredicted gene on human chromosome 21 (21q21.2), encodes a cysteine and tyrosine-rich protein and defines a new family of highly conserved vertebrate-specific genes. Gene 290: 141-151.
39. Vitale L, Frabetti F, Huntsman SA, Canaider S, Casadei R, et al. (2007) Sequence, "subtle" alternative splicing and expression of the CYYR1 (cysteine/tyrosine-rich 1) mRNA in human neuroendocrine tumors. BMC cancer 7: 66.
40. Peh GS, Lee MX, Wu FY, Toh KP, Balehosur D, et al. (2012) Optimization of human corneal endothelial cells for culture: the removal of corneal stromal fibroblast contamination using magnetic cell separation. International journal of biomaterials 2012: 601302.
41. Hay ED, (1980) Development of the vertebrate cornea. International review of cytology 63: 263-322.
42. Lwigale PY, Cressy PA, Bronner-Fraser M, (2005) Corneal keratocytes retain neural crest progenitor cell properties. Developmental biology 288: 284-293.
43. Joyce NC, Harris DL, Mello DM, (2002) Mechanisms of mitotic inhibition in corneal endothelium: contact inhibition and TGF-beta2. Investigative ophthalmology & visual science 43: 2152-2159.
44. Ittner LM, Wurdak H, Schwerdtfeger K, Kunz T, Ille F, et al. (2005) Compound developmental eye disorders following inactivation of TGFbeta signaling in neural-crest stem cells. Journal of biology 4: 11.
45. Tumer Z, Bach-Holm D, (2009) Axenfeld-Rieger syndrome and spectrum of PITX2 and FOXC1 mutations. European journal of human genetics: EJHG 17: 1527-1539.
46. Zacharias AL, Gage PJ, (2010) Canonical Wnt/beta-catenin signaling is required for maintenance but not activation of Pitx2 expression in neural crest during eye development. Developmental dynamics: an official publication of the American Association of Anatomists 239: 3215-3225.
47. Gage PJ, Qian M, Wu D, Rosenberg KI, (2008) The canonical Wnt signaling antagonist DKK2 is an essential effector of PITX2 function during normal eye development. Developmental biology 317: 310-324.
48. Peh GS, Toh KP, Wu FY, Tan DT, Mehta JS, (2011) Cultivation of human corneal endothelial cells isolated from paired donor corneas. PloS one 6: e28310.
49. Peh GS, Toh KP, Ang HP, Seah XY, George BL, et al. (2013) Optimization of human corneal endothelial cell culture: density dependency of successful cultures in vitro. BMC research notes 6: 176.