Optimization of human corneal endothelial cells for culture: The removal of corneal stromal fibroblast contamination using magnetic cell separation

International Journal of Biomaterials

Volumn , Issue , 2012, Pages

  Peh, Gary S L ^a   Lee, Man Xin ^a   Wu, Fei Yi ^a   Toh, Kah Peng ^a   Balehosur, Deepashree ^a   Mehta, Jodhbir S ^a,b,c,d  

^a SINGAPORE EYE RESEARCH INSTITUTE   (Singapore)

^b SINGAPORE NATIONAL EYE CENTRE   (Singapore)

^c DUKE NUS MEDICAL SCHOOL   (Singapore)

^d NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84863014263     PISSN: 16878787     EISSN: 16878795     Source Type: Journal    
DOI: 10.1155/2012/601302     Document Type: Article

References (38)

1. Maurice D. M., The location of the fluid pump in the cornea Journal of Physiology 1972 221 1 43 54
2. Fischbarg J., Lim J. J., Role of cations, anions and carbonic anhydrase in fluid transport across rabbit corneal endothelium Journal of Physiology 1974 241 3 647 675
3. Bourne W. M., Clinical estimation of corneal endothelial pump function Transactions of the American Ophthalmological Society 1998 96 229 242 (Pubitemid 29012915)
4. Carlson K. H., Bourne W. M., McLaren J. W., Brubaker R. F., Variations in human corneal endothelial cell morphology and permeability to fluorescein with age Experimental Eye Research 1988 47 1 27 41
5. Bourne W. M., Nelson L. I. L., Hodge D. O., Central corneal endothelial cell changes over a ten-year period Investigative Ophthalmology and Visual Science 1997 38 3 779 782 (Pubitemid 27143150)
6. Murphy C., Alvarado J., Juster R., Maglio M., Prenatal and postnatal cellularity of the human corneal endothelium. A quantitative histologic study Investigative Ophthalmology and Visual Science 1984 25 3 312 322 (Pubitemid 14164779)
7. Williams K. K., Noe R. L., Grossniklaus H. E., Drews-Botsch C., Edelhauser H. F., Correlation of histologic corneal endothelial cell counts with specular microscopic cell density Archives of Ophthalmology 1992 110 8 1146 1149
8. Peh G. S.L., Beuerman R. W., Colman A., Tan D. T., Mehta J. S., Human corneal endothelial cell expansion for corneal endothelium transplantation: an overview Transplantation 2011 91 8 811 819
9. Armitage W. J., Moss S. J., Easty D. L., Bradley B. A., Supply of corneal tissue in the United Kingdom British Journal of Ophthalmology 1990 74 11 685 687 (Pubitemid 20365697)
10. McColgan K., Corneal transplant surgery Journal of perioperative practice 2009 19 2 51 54
11. Ruberti J. W., Zieske J. D., Prelude to corneal tissue engineeringGaining control of collagen organization Progress in Retinal and Eye Research 2008 27 5 549 577
12. Engelmann K., Bednarz J., Valtink M., Prospects for endothelial transplantation Experimental Eye Research 2004 78 3 573 578 (Pubitemid 38183336)
13. Joyce N. C., Zhu C. C., Human corneal endothelial cell proliferation: potential for use in regenerative medicine Cornea 2004 23 8 S8 S19
14. Baum J. L., Niedra R., Davis C., Yue B. T., Mass culture of human corneal endothelial cells Archives of Ophthalmology 1979 97 6 1136 1140 (Pubitemid 9187849)
15. Bednarz J., Doubilei V., Wollnik P. C. M., Engelmann K., Effect of three different media on serum free culture of donor corneas and isolated human corneal endothelial cells British Journal of Ophthalmology 2001 85 12 1416 1420 (Pubitemid 34032904)
16. Chen K. H., Azar D., Joyce N. C., Transplantation of adult human corneal endothelium ex vivo: a morphologic study Cornea 2001 20 7 731 737 (Pubitemid 34506472)
17. Fabricant R. N., Alpar A. J., Centifanto Y. M., Kaufman H. E., Epidermal growth factor receptors on corneal endothelium Archives of Ophthalmology 1981 99 2 305 308 (Pubitemid 11184149)
18. Ishino Y., Sano Y., Nakamura T., Connon C. J., Rigby H., Fullwood N. J., Kinoshita S., Amniotic membrane as a carrier for cultivated human corneal endothelial cell transplantation Investigative Ophthalmology and Visual Science 2004 45 3 800 806 (Pubitemid 38868373)
19. Tripathi R. C., Tripathi B. J., Human trabecular endothelium, corneal endothelium, keratocytes, and scleral fibroblasts in primary cell culture. A comparative study of growth characteristics, morphology, and phagocytic activity by light and scanning electron microscopy Experimental Eye Research 1982 35 6 611 624 (Pubitemid 13223637)
20. Yue B. Y., Sugar J., Gilboy J. E., Elvart J. L., Growth of human corneal endothelial cells in culture Investigative Ophthalmology and Visual Science 1989 30 2 248 253 (Pubitemid 19055602)
21. Li W., Sabater A. L., Chen Y. T., Hayashida Y., Chen S. Y., He H., Tseng S. C. G., A novel method of isolation, preservation, and expansion of human corneal endothelial cells Investigative Ophthalmology and Visual Science 2007 48 2 614 620 (Pubitemid 351260692)
22. West-Mays J. A., Dwivedi D. J., The keratocyte: corneal stromal cell with variable repair phenotypes International Journal of Biochemistry and Cell Biology 2006 38 10 1625 1631 (Pubitemid 43947647)
23. Engelmann K., Bohnke M., Friedl P., Isolation and long-term cultivation of human corneal endothelial cells Investigative Ophthalmology and Visual Science 1988 29 11 1656 1662
24. Edelhauser H. F., The resiliency of the corneal endothelium to refractive and intraocular surgery Cornea 2000 19 3 263 273 (Pubitemid 30322446)
25. Grtzkau A., Radbruch A., Small but mighty: how the MACS1-technology based on nanosized superparamagnetic particles has helped to analyze the immune system within the last 20 years Cytometry A 2010 77 7 643 647
26. Miltenyi S., Muller W., Weichel W., Radbruch A., High gradient magnetic cell separation with MACS Cytometry 1990 11 2 231 238 (Pubitemid 20097230)
27. Fong C. Y., Peh G., Subramanian A., Gauthaman K., Bongso A., The use of discontinuous density gradients in stem cell research and application Stem Cell Reviews and Reports 2010 5 4 428 434
28. Klyce S. D., Beuerman R. W., Kaufman H. E., Barron B. A., McDonald M. B., Waltman S. R., Structure and function of the cornea The Cornea 1988 New York, NY, USA Churchill Livingstone 3 28
29. Yee R. W., Matsuda M., Schultz R. O., Edelhauser H. F., Changes in the normal corneal endothelial cellular pattern as a function of age Current Eye Research 1985 4 6 671 678 (Pubitemid 15071017)
30. Engelmann K., Friedl P., Growth of human corneal endothelial cells in a serum-reduced medium Cornea 1995 14 1 62 70
31. Joyce N. C., Proliferative capacity of the corneal endothelium Progress in Retinal and Eye Research 2003 22 3 359 389 (Pubitemid 38352553)
32. Bednarz J., Different characteristics of endothelial cells from central and peripheral human cornea in primary culture and after subculture In Vitro Cellular and Developmental BiologyAnimal 1998 34 2 149 153
33. Mimura T., Joyce N. C., Replication competence and senescence in central and peripheral human corneal endothelium Investigative Ophthalmology and Visual Science 2006 47 4 1387 1396 (Pubitemid 46780745)
34. Gilbert S. F., Migeon B. R., D valine as a selective agent for normal human and rodent epithelial cells in culture Cell 1975 5 1 11 17
35. Zhu C., Joyce N. C., Proliferative response of corneal endothelial cells from young and older donors Investigative Ophthalmology and Visual Science 2004 45 6 1743 1751 (Pubitemid 38868967)
36. Zahler S., Kowalski C., Brosig A., Kupatt C., Becker B. F., Gerlach E., The function of neutrophils isolated by a magnetic antibody cell separation technique is not altered in comparison to a density gradient centrifugation method Journal of Immunological Methods 1997 200 1-2 173 179 (Pubitemid 27035699)
37. Geroski D. H., Matsuda M., Yee R. W., Edelhauser H. F., Pump function of the human corneal endothelium. Effects of age and cornea guttata Ophthalmology 1985 92 6 759 763 (Pubitemid 15060003)
38. McCartney M. D., Wood T. O., McLaughlin B. J., Immunohistochemical localization of ATPase in human dysfunctional corneal endothelium Current Eye Research 1987 6 12 1479 1488 (Pubitemid 18027498)