Microdissection and culture of murine pancreatic ductal epithelial cells

Methods in Molecular Biology

Volumn 980, Issue , 2013, Pages 267-279

  Pylayeva Gupta, Yuliya ^a   Lee, Kyoung Eun ^a   Bar Sagi, Dafna ^a  

^a NONE

Author keywords

3D epithelial cell culture system; Matrigel embedding; Pancreatic cancer; Pancreatic ductal epithelial cells

Indexed keywords

ANIMAL; ARTICLE; CELL TRANSFORMATION; CYTOLOGY; EPITHELIUM CELL; METHODOLOGY; MICRODISSECTION; MOUSE; PANCREAS DUCT; PRIMARY CELL CULTURE;

ANIMALS; CELL TRANSFORMATION, NEOPLASTIC; EPITHELIAL CELLS; MICE; MICRODISSECTION; PANCREATIC DUCTS; PRIMARY CELL CULTURE;

MLCS; MLOWN;

EID: 84879582311     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-287-2_14     Document Type: Article

References (9)

1. Maitra A, Hruban RH (2008) Pancreatic cancer. Annu Rev Pathol 3:157-188
2. Hingorani SR, Petricoin EF, Maitra A, Rajapakse V, King C, Jacobetz MA, Ross S, Conrads TP, Veenstra TD, Hitt BA, Kawaguchi Y, Johann D, Liotta LA, Crawford HC, Putt ME, Jacks T, Wright CV, Hruban RH, Lowy AM, Tuveson DA (2003) Preinvasive and invasive ductal pancreatic cancer and its early detection in the mouse. Cancer Cell 4:437-450
3. Guerra C, Schuhmacher AJ, Canamero M, Grippo PJ, Verdaguer L, Perez-Gallego L, Dubus P, Sandgren EP, Barbacid M (2007) Chronic pancreatitis is essential for induction of pancreatic ductal adenocarcinoma by K-Ras oncogenes in adult mice. Cancer Cell 11:291-302
4. Agbunag C, Lee KE, Buontempo S, Bar-Sagi D (2006) Pancreatic duct epithelial cell isolation and cultivation in two-dimensional and three-dimensional culture systems. Methods Enzymol 407:703-710
5. Hingorani SR, Wang L, Multani AS, Combs C, Deramaudt TB, Hruban RH, Rustgi AK, Chang S, Tuveson DA (2005) Trp53R172H and KrasG12D cooperate to promote chromosomal instability and widely metastatic pancreatic ductal adenocarcinoma in mice. Cancer Cell 7:469-483
6. Aguirre AJ, Bardeesy N, Sinha M, Lopez L, Tuveson DA, Horner J, Redston MS, DePinho RA (2003) Activated Kras and Ink4a/Arf deficiency cooperate to produce metastatic pancreatic ductal adenocarcinoma. Genes Dev 17:3112-3126
7. Jackson EL, Willis N, Mercer K, Bronson RT, Crowley D, Montoya R, Jacks T, Tuveson DA (2001) Analysis of lung tumor initiation and progression using conditional expression of oncogenic K-ras. Genes Dev 15:3243-3248
8. Lee KE, Bar-Sagi D (2010) Oncogenic KRas suppresses inflammation- associated senescence of pancreatic ductal cells. Cancer Cell 18:448-458
9. Debnath J, Brugge JS (2005) Modelling glandular epithelial cancers in three-dimensional cultures. Nat Rev Cancer 5:675-688