Saponin promotes rapid identification and antimicrobial susceptibility profiling of Gram-positive and Gram-negative bacteria in blood cultures with the Vitek 2 system

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 32, Issue 4, 2013, Pages 493-502

  Lupetti, A ^a   Barnini, S ^a   Morici, P ^a   Ghelardi, E ^a   Nibbering, P H ^b   Campa, M ^a  

^a UNIVERSITY OF PISA   (Italy)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

AMIKACIN; AMPICILLIN; BETA LACTAM ANTIBIOTIC; CEFAZOLIN; CEFEPIME; CEFOTAXIME; CEFTAZIDIME; CIPROFLOXACIN; CLINDAMYCIN; COTRIMOXAZOLE; ERYTHROMYCIN; GENTAMICIN; IMIPENEM; LEVOFLOXACIN; LINEZOLID; MEROPENEM; OXACILLIN; PENICILLIN DERIVATIVE; PIPERACILLIN PLUS TAZOBACTAM; RIFAMPICIN; SAPONIN; TEICOPLANIN; TETRACYCLINE; TIGECYCLINE; TOBRAMYCIN; VANCOMYCIN;

ACCURACY; ANALYTICAL ERROR; ANTIBIOTIC SENSITIVITY; ARTICLE; AUTOANALYSIS; BACILLUS CEREUS; BACILLUS MEGATERIUM; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BLOOD CULTURE; COMPUTER PROGRAM; CONTROLLED STUDY; CULTURE MEDIUM; GEMELLA MORBILLORUM; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; HUMAN; INOCULATION; INTERMETHOD COMPARISON; KOCURIA KRISTINAE; MICROCOCCUS LUTEUS; MINIMUM INHIBITORY CONCENTRATION; MORAXELLA CATARRHALIS; NONHUMAN; OLIGELLA UREOLYTICA; PRIORITY JOURNAL; PROSPECTIVE STUDY; RAPID TEST; RELIABILITY; SPHINGOMONAS PAUCIMOBILIS; STAPHYLOCOCCUS EPIDERMIDIS; STREPTOCOCCUS; STREPTOCOCCUS ORALIS; STREPTOCOCCUS PLURANIMALUM; STREPTOCOCCUS PYOGENES;

BACTEREMIA; BACTERIAL TYPING TECHNIQUES; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE BACTERIA; HUMANS; MICROBIAL SENSITIVITY TESTS; SAPONINS; SPECIMEN HANDLING; TIME FACTORS;

EID: 84879463031     PISSN: 09349723     EISSN: 14354373     Source Type: Journal    
DOI: 10.1007/s10096-012-1762-z     Document Type: Article

References (36)

1. Bearman GML, Wenzel RP (2005) Bacteremias: a leading cause of death. Arch Med Res 36:646-659. doi: 10.1016/j.arcmed.2005.02.005 (Pubitemid 41436234)
2. Martin GS, Mannino DM, Eaton S, Moss M (2003) The epidemiology of sepsis in the United States from 1979 through 2000. N Engl J Med 348:1546-1554 (Pubitemid 36437931)
3. Ibrahim EH, Sherman G, Ward S, Fraser VJ, Kollef MH (2000) The influence of inadequate antimicrobial treatment of bloodstream infections on patient outcomes in the ICU setting. Chest 118:146-155 (Pubitemid 30485502)
4. Doern GV, Vautour R, Gaudet M, Levy B (1994) Clinical impact of rapid in vitro susceptibility testing and bacterial identification. J Clin Microbiol 32:1757-1762 (Pubitemid 24189570)
5. Barenfanger J, Drake C, Kacich G (1999) Clinical and financial benefits of rapid bacterial identification and antimicrobial susceptibility testing. J Clin Microbiol 37:1415-1418 (Pubitemid 29187810)
6. Hrabák J, Studentová V, Walková R, Zemlicková H, Jakubu V, Chudácková E, Gniadkowski M, Pfeifer Y, Perry JD, Wilkinson K, Bergerová T (2012) Detection of NDM-1, VIM-1, KPC, OXA-48, and OXA-162 carbapenemases by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 50:2441-2443. doi: 10.1128/JCM.01002-12
7. Sparbier K, Schubert S, Weller U, Boogen C, Kostrzewa M (2012) Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based functional assay for rapid detection of resistance against β-lactam antibiotics. J Clin Microbiol 50:927-937. doi: 10.1128/JCM.05737-11
8. Hooff GP, van Kampen JJ, Meesters RJ, van Belkum A, Goessens WH, Luider TM (2012) Characterization of β-lactamase enzyme activity in bacterial lysates using MALDI-mass spectrometry. J Proteome Res 11:79-84. doi: 10.1021/pr200858r
9. Bruins MJ, Bloembergen P, Ruijs GJ, Wolfhagen MJ (2004) Identification and susceptibility testing of Enterobacteriaceae and Pseudomonas aeruginosa by direct inoculation from positive BACTEC blood culture bottles into Vitek 2. J Clin Microbiol 42:7-11. doi: 10.1128/JCM.42.1.7-11.2004 (Pubitemid 38082691)
10. Chen J-R, Lee S-Y, Yang BH, Lu JJ (2008) Rapid identification and susceptibility testing using the Vitek 2 system using culture fluids from positive BacT/Alert blood cultures. J Microbiol Immunol Infect 41:259-264
11. de Cueto M, Ceballos E, Martinez-Martinez L, Perea EJ, Pascual A (2004) Use of positive blood cultures for direct identification and susceptibility testing with the Vitek 2 system. J Clin Microbiol 42:3734-3738. doi: 10.1128/JCM.42.8.3734-3738.2004 (Pubitemid 39045706)
12. Fontanals D, Salceda F, Hernández J, Sanfeliu I, Torra M (2002) Evaluation of wider system for direct identification and antimicrobial susceptibility testing of gram-negative bacilli from positive blood culture bottles. Eur J Clin Microbiol Infect Dis 21:693-695. doi: 10.1007/s10096-002- 0791-4
13. Funke G, Funke-Kissling P (2004) Use of the BD Phoenix Automated Microbiology System for direct identification and susceptibility testing of gram-negative rods from positive blood cultures in a three-phase trial. J Clin Microbiol 42:1466-1470. doi: 10.1128/JCM.42.4.1466-1470.2004 (Pubitemid 38500782)
14. Hansen DS, Jensen AG, Nørskov-Lauritsen N, Skov R, Bruun B (2002) Direct identification and susceptibility testing of enteric bacilli from positive blood cultures using Vitek (GNI+/GNS-GA). Clin Microbiol Infect 8:38-44. doi: 10.1046/j.1469-0691.2002.00372.x (Pubitemid 34993758)
15. Ling TK, Liu ZK, Cheng AF (2003) Evaluation of the Vitek 2 system for rapid direct identification and susceptibility testing of gram-negative bacilli from positive blood cultures. J Clin Microbiol 41:4705-4707. doi: 10.1128/JCM.41.10.4705-4707.2003 (Pubitemid 37259781)
16. Waites KB, Brookings ES, Moser SA, Zimmer BL (1998) Direct susceptibility testing with positive BacT/Alert blood cultures by using MicroScan overnight and rapid panels. J Clin Microbiol 36:2052-2056 (Pubitemid 28277801)
17. Waites KB, Brookings ES, Moser SA, Zimmer BL (1998) Direct bacterial identification from positive BacT/Alert blood cultures using MicroScan overnight and rapid panels. Diagn Microbiol Infect Dis 32:21-26. doi: 10.1016/S0732-8893(98)00058-3 (Pubitemid 29168523)
18. Munoz-Dávila MJ, Yagüe G, Albert M, García-Lucas T (2012) Comparative evaluation of Vitek 2 identification and susceptibility testing of Gram-negative rods directly and isolated from BacT/ALERT-positive blood culture bottles. Eur J Clin Microbiol Infect Dis 31:663-669. doi: 10.1007/s10096-011-1356-1
19. Lupetti A, Barnini S, Castagna B, Capria AL, Nibbering PH (2010) Rapid identification and antimicrobial susceptibility profiling of Gram-positive cocci in blood cultures with the Vitek 2 system. Eur J Clin Microbiol Infect Dis 29:89-95. doi: 10.1007/s10096-009-0825-2
20. Lupetti A, Barnini S, Castagna B, Nibbering PH, Campa M (2010) Rapid identification and antimicrobial susceptibility testing of Gram-positive cocci in blood cultures by direct inoculation into the BD Phoenix system. Clin Microbiol Infect 16:986-991. doi: 10.1111/j.1469-0691.2009.03006.x
21. Clinical and Laboratory Standards Institute (CLSI) (2006) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard - seventh edition. CLSI document M7-A7. CLSI, Wayne, PA
22. Clinical and Laboratory Standards Institute (CLSI) (2007) Performance standards for antimicrobial susceptibility testing; 17th informational supplement. CLSI document M100-S17. CLSI, Wayne, PA
23. Murray PR, Spizzo AW, Niles AC (1991) Clinical comparison of the recoveries of bloodstream pathogens in Septi-Chek brain heart infusion broth with saponin, Septi-Chek tryptic soy broth, and the isolator lysis-centrifugation system. J Clin Microbiol 29:901-905
24. Jorgensen JH (1993) Selection criteria for an antimicrobial susceptibility testing system. J Clin Microbiol 31:2841-2844 (Pubitemid 23318365)
25. Christner M, Rohde H, Wolters M, Sobottka I, Wegscheider K, Aepfelbacher M (2010) Rapid identification of bacteria from positive blood culture bottles by use of matrix-assisted laser desorption-ionization time of flight mass spectrometry fingerprinting. J Clin Microbiol 48:1584-1591. doi: 10.1128/JCM.01831-09
26. Ferroni A, Suarez S, Beretti JL, Dauphin B, Bille E, Meyer J, Bougnoux ME, Alanio A, Berche P, Nassif X (2010) Real-time identification of bacteria and Candida species in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 48:1542-1548. doi: 10.1128/JCM.02485-09
27. Prod'hom G, Bizzini A, Durussel C, Bille J, Greub G (2010) Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets. J Clin Microbiol 48:1481-1483. doi: 10.1128/JCM.01780-09
28. Stevenson LG, Drake SK, Murray PR (2010) Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 48:444-447. doi: 10.1128/JCM.01541-09
29. Peters RP, de Boer RF, Schuurman T, Gierveld S, Kooistra-Smid M, van Agtmael MA, Vandenbroucke-Grauls CM, Persoons MC, Savelkoul PH (2009) Streptococcus pneumoniae DNA load in blood as a marker of infection in patients with community-acquired pneumonia. J Clin Microbiol 47:3308-3312. doi: 10.1128/JCM.01071-09
30. Kee C, Palladino S, Kay I, Pryce TM, Murray R, Rello J, Gallego M, Lujan M, Muñoz-Almagro C, Waterer GW (2008) Feasibility of real-time polymerase chain reaction in whole blood to identify Streptococcus pneumoniae in patients with community-acquired pneumonia. Diagn Microbiol Infect Dis 61:72-75. doi: 10.1016/j.diagmicrobio.2007.12.011 (Pubitemid 351509748)
31. Forrest GN, Roghmann MC, Toombs LS, Johnson JK, Weekes E, Lincalis DP, Venezia RA (2008) Peptide nucleic acid fluorescent in situ hybridization for hospital-acquired enterococcal bacteremia: delivering earlier effective antimicrobial therapy. Antimicrob Agents Chemother 52:3558-3563. doi: 10.1128/AAC.00283-08
32. Stanley KK, Szewczuk E (2005) Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection. Nucleic Acids Res 33:e180. doi: 10.1093/nar/gni182 (Pubitemid 41806408)
33. Lau A, Sorrell TC, Chen S, Stanley K, Iredell J, Halliday C (2008) Multiplex tandem PCR: a novel platform for rapid detection and identification of fungal pathogens from blood culture specimens. J Clin Microbiol 46:3021-3027. doi: 10.1128/JCM.00689-08
34. Ellem J, Partridge SR, Iredell JR (2011) Efficient direct extended-spectrum β-lactamase detection by multiplex real-time PCR: accurate assignment of phenotype by use of a limited set of genetic markers. J Clin Microbiol 49:3074-3077. doi: 10.1128/JCM.02647-10
35. CTX-M within an Australian extended-spectrum beta-lactamase gene pool. Antimicrob Agents Chemother 52:4198-4202. doi: 10.1128/AAC.00107-08
36. Fishbain JT, Sinyavskiy O, Riederer K, Hujer AM, Bonomo RA (2012) Detection of extended-spectrum β-lactamase and Klebsiella pneumoniae carbapenemase genes directly from blood cultures by use of a nucleic acid microarray. J Clin Microbiol 50(9):2901-2904. doi: 10.1128/JCM.01023-12