Validation of a quantitative real-time PCR assay and comparison with fluorescence microscopy and selective agar plate counting for species-specific quantification of an in vitro subgingival biofilm model

Journal of Periodontal Research

Volumn 48, Issue 4, 2013, Pages 517-526

  Ammann, T W ^a   Bostanci, N ^a   Belibasakis, G N ^a   Thurnheer, T ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

Fluorescence in situ hybridization; Immunofluorescence; Quantitative real time PCR; Subgingival biofilm

Indexed keywords

AGAR;

ACTINOMYCES; ARTICLE; BACTERIAL LOAD; BACTEROIDES; BIOFILM; CAMPYLOBACTER RECTUS; CLASSIFICATION; COMPARATIVE STUDY; CULTURE MEDIUM; FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENCE MICROSCOPY; FLUORESCENT ANTIBODY TECHNIQUE; FUSOBACTERIUM NUCLEATUM; GINGIVA; GROWTH, DEVELOPMENT AND AGING; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; PORPHYROMONAS GINGIVALIS; PREVOTELLA INTERMEDIA; REAL TIME POLYMERASE CHAIN REACTION; STREPTOCOCCUS ANGINOSUS; STREPTOCOCCUS ORALIS; TIME; TREPONEMA DENTICOLA; VALIDATION STUDY; VEILLONELLA;

ACTINOMYCES; AGAR; BACTERIAL LOAD; BACTERIOLOGICAL TECHNIQUES; BACTEROIDES; BIOFILMS; CAMPYLOBACTER RECTUS; CULTURE MEDIA; FLUORESCENT ANTIBODY TECHNIQUE; FUSOBACTERIUM NUCLEATUM; GINGIVA; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROSCOPY, FLUORESCENCE; PORPHYROMONAS GINGIVALIS; PREVOTELLA INTERMEDIA; REAL-TIME POLYMERASE CHAIN REACTION; STREPTOCOCCUS ANGINOSUS; STREPTOCOCCUS ORALIS; TIME FACTORS; TREPONEMA DENTICOLA; VEILLONELLA;

EID: 84879461717     PISSN: 00223484     EISSN: 16000765     Source Type: Journal    
DOI: 10.1111/jre.12034     Document Type: Article

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