Multiplex FISH analysis of a six-species bacterial biofilm

Journal of Microbiological Methods

Volumn 56, Issue 1, 2004, Pages 37-47

  Thurnheer, T ^a   Gmur R ^a   Guggenheim, B ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

16S rRNA; Biofilm; CLSM; Fluorescent in situ hybridization

Indexed keywords

6 CARBOXYFLUORESCEIN; CYSTEINE; FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; HYDROXYAPATITE; RED FLUORESCENT PROTEIN;

ACTINOMYCES NAESLUNDII; AMINO ACID SEQUENCE; ANAEROBIC METABOLISM; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BIOFILM; CANDIDA ALBICANS; COMPLEMENT FIXATION; CONFOCAL LASER MICROSCOPY; CULTURE MEDIUM; EXPOSURE; FLUORESCENCE IN SITU HYBRIDIZATION; FUSOBACTERIUM NUCLEATUM; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; GRAM STAINING; HYBRIDIZATION; LYSOSOME; MOUTH FLORA; NONHUMAN; OLIGONUCLEOTIDE PROBE; PERMEABILITY; PRIORITY JOURNAL; SALIVA LEVEL; STREPTOCOCCUS ORALIS; STREPTOCOCCUS SOBRINUS; TARGET CELL; VEILLONELLA; VEILLONELLA DISPAR;

ACTINOMYCES NAESLUNDII; BACTERIA (MICROORGANISMS); CANDIDA ALBICANS; FUSOBACTERIUM NUCLEATUM; NEGIBACTERIA; POSIBACTERIA; STREPTOCOCCUS ORALIS; STREPTOCOCCUS SOBRINUS; VEILLONELLA DISPAR;

EID: 0346099178     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2003.09.003     Document Type: Article

References (30)

1. Amann R.I., Binder B.J., Olson R.J., Chisholm S.W., Devereux R., Stahl D.A. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56:1990;1919-1925.
2. Amann R.I., Ludwig W., Schleifer K.-H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:1995;143-169.
3. Amann R., Snaidr J., Wagner M., Ludwig W., Schleifer K.H. In situ visualization of high genetic diversity in a natural microbial community. J. Bacteriol. 178:1996;3496-3500.
4. Beimfohr C., Krause A., Amann R., Ludwig W., Schleifer K.-H. In situ identification of Lactococci Enterococci and Streptococci. Syst. Appl. Microbiol. 16:1993;450-456.
5. Brelje T.C., Wessendorf M.W., Sorenson R.L. Multicolor laser scanning confocal immunofluorescence microscopy: practical application and limitations. Methods Cell Biol. 38:1993;97-181.
6. Costerton J.W., Lewandowski Z., Caldwell D.E., Korber D.R., Lappin-Scott H.M. Microbial biofilms. Annu. Rev. Microbiol. 49:1995;711-745.
7. Davey M.E., O'Toole G.A. Microbial biofilms: from ecology to molecular genetics. Microbiol. Mol. Biol. Rev. 64:2000;847-867.
8. Gmür R., Guggenheim B. Antigenic heterogeneity of Bacteroides intermedius as recognized by monoclonal antibodies. Infect. Immun. 42:1983;459-470.
9. Guggenheim B., Giertsen E., Schüpbach P., Shapiro S. Validation of an in vitro biofilm model of supragingival plaque. J. Dent. Res. 80:2001;363-370.
10. Guggenheim M., Shapiro S., Gmür R., Guggenheim B. Spatial arrangements and associative behavior of species in an in vitro oral biofilm model. Appl. Environ. Microbiol. 67:2001;1343-1350.
11. Hermanowicz S.W. Biofilm structure: an interplay of models and experiments. Wuertz S., Bishop P.L., Wilderer P.A. Biofilms in wastewater treatment - an interdisciplinary approach. 2003;32-47 International Water Association Publishing, London, United Kingdom.
12. Lawrence J.R., Korber D.R., Hoyle B.D., Costerton J.W., Caldwell D.E. Optical sectioning of microbial biofilms. J. Bacteriol. 173:1991;6558-6567.
13. Maeda H., Ishida N. Specificity of binding of hexopyranosyl polysaccharides with fluorescent brightener. J. Biochem. 62:1967;276-278.
14. Maidak B.L., Cole J.R., Parker C.T. Jr., Garrity G.M., Larsen N., Li B., Lilburn T.G., McCaughey M.J., Olsen G.J., Overbeek R., Pramanik S., Schmidt T.M., Tiedje J.M., Woese C.R. A new version of the RDP (Ribosomal Database Project). Nucleic Acids Res. 27:1999;171-173.
15. Manz W., Amann R., Ludwig W., Wagner M., Schleifer K.-H. Phylogenetic oligodeoxynucleotide probes for the major subclasses of proteobacteria: problems and solutions. Syst. Appl. Microbiol. 15:1992;593-600.
16. Manz W., Eisenbrecher M., Neu T.R., Szewzyk U. Abundance and spatial organization of Gram-negative sulfate-reducing bacteria in activated sludge investigated by in situ probing with specific 16S rRNA targeted oligonucleotides. FEMS Microbiol. Ecol. 25:1998;43-61.
17. Marsh P.D., Bradshaw D.J. Dental plaque as a biofilm. J. Ind. Microbiol. 15:1995;169-175.
18. Moter A., Göbel U. Fluorescence in situ hybridization (FISH) for direct visualization of microorganism. J. Microbiol. Methods. 41:2000;85-112.
19. Neu T.R., Kuhlicke U., Lawrence J.R. Assessment of fluorochromes for two-photon laser scanning microscopy of biofilms. Appl. Environ. Microbiol. 68:2002;901-909.
20. Okabe S., Satoh H., Watanabe Y. In situ analysis of nitrifying biofilms as determined by in situ hybridization and the use of microelectrodes. Appl. Environ. Microbiol. 65:1999;3182-3191.
21. Paster B.J., Bartoszyk I.M., Dewhirst F.E. Identification of oral streptococci using PCR-based, reverse-capture, checkerboard hybridization. Methods Cell Sci. 20:1998;223-231.
22. Perry-O'Keefe H., Rigby S., Oliveira K., Sorensen D., Slender H., Coull J., Hyldig-Nielsen J.J. Identification of indicator microorganisms using a standardized PNA FISH method. J. Microbiol. Methods. 47:2001;281-292.
23. Roller C., Wagner M., Amann R., Ludwig W., Schleifer K.-H. In situ probing of gram-positive bacteria with high DNA G+C content using 23S rRNA-targeted oligonucleotides. Microbiology. 140:1994;2849-2858.
24. Schmid M., Twachtmann U., Klein M., Strous M., Juretschko S., Jetten M., Metzger J.W., Schleifer K.H., Wagner M. Molecular evidence for genus level diversity of bacteria capable of catalyzing anaerobic ammonium oxidation. Syst. Appl. Microbiol. 23:2000;93-106.
25. Shapiro S., Giertsen E., Guggenheim B. An in vitro oral biofilm model for comparing efficacies of antimicrobial mouthrinses. Caries Res. 36:2002;93-100.
26. Thurnheer T., Gmür R., Giertsen E., Guggenheim B. Automated fluorescent in situ hybridization for the specific detection and quantification of oral streptococci in dental plaque. J. Microbiol. Methods. 44:2001;39-47.
27. Thurnheer T., Gmür R., Shapiro S., Guggenheim B. Mass transport of macromolecules within an in vitro model of supragingival plaque. Appl. Environ. Microbiol. 69:2003;1702-1709.
28. Wagner M., Amann R., Kämpfer P., Assmus B., Hartmann A., Hutzler P., Springer N., Schleifer K.-H. Identification and in situ detection of Gram-negative filamentous bacteria in activated sludge. Syst. Appl. Microbiol. 17:1994;405-417.
29. Wecke J., Kersten T., Madela K., Moter A., Göbel U.B., Friedmann A., Bernimoulin J.P. A novel technique for monitoring the development of bacterial biofilms in human periodontal pockets. FEMS Microbiol. Lett. 191:2000;95-101.
30. Wimpenny J., Manz W., Szewzyk U. Heterogeneity in biofilms. FEMS Microbiol. Rev. 24:2000;661-671.