Identification of two novel BTV16-specific B cell epitopes using monoclonal antibodies against the VP2 protein

Applied Microbiology and Biotechnology

Volumn 97, Issue 13, 2013, Pages 5933-5942

  Wang, Wen Shi ^a   Sun, En Cheng ^a   Xu, Qing Yuan ^a   Yang, Tao ^a   Qin, Yong Li ^a   Zhao, Jing ^a   Feng, Yu Fei ^a   Li, Jun Ping ^a   Wei, Peng ^a   Zhang, Cui Yun ^a   Wu, Dong Lai ^a  

^a INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Bluetongue virus; Epitopes; Serotype specific monoclonal antibody; VP2 protein

Indexed keywords

AMINO ACID SEQUENCE; ANTIBODY BINDING; BLUETONGUE; DIAGNOSTIC TOOLS; MANNOSE-BINDING PROTEINS; MONOCLONAL ANTIBODIES (MABS); SEQUENCE ALIGNMENTS; STRUCTURAL PROTEINS;

ALIGNMENT; AMINO ACIDS; MONOCLONAL ANTIBODIES; PEPTIDES; SURFACE PLASMON RESONANCE; VIRUSES;

EPITOPES;

EPITOPE; HYBRID PROTEIN; MANNOSE BINDING PROTEIN; MONOCLONAL ANTIBODY; PROTEIN VP2; VIRUS VACCINE;

AMINO ACID; ANTIBODY; CELL ORGANELLE; IDENTIFICATION METHOD; LINEARITY; PROTEIN; VIRUS;

AMINO ACID SEQUENCE; ANIMAL CELL; ANTIGEN BINDING; ARTICLE; B LYMPHOCYTE; BLUETONGUE ORBIVIRUS; CONTROLLED STUDY; CROSS REACTION; DRUG SYNTHESIS; EPITOPE MAPPING; GENETIC CONSERVATION; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE SEQUENCE; PEPTIDE SYNTHESIS; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN EXPRESSION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; SEROTYPE; VIRUS ISOLATION; VIRUS STRAIN;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; ANTIBODIES, VIRAL; BLUETONGUE VIRUS; CAPSID PROTEINS; EPITOPE MAPPING; EPITOPES, B-LYMPHOCYTE; PROTEIN BINDING; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SEROTYPING;

BLUETONGUE VIRUS; ORBIVIRUS; REOVIRIDAE;

EID: 84879409906     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-4779-z     Document Type: Article

References (20)

1. Bonneau KR, Mullens BA, MacLachlan NJ (2001) Occurrence of genetic drift and founder effect during quasispecies evolution of the VP2 and NS3/NS3A genes of bluetongue virus upon passage between sheep, cattle, and Culicoides sonorensis. J Virol 75:8298-8305
2. Boone JD, Balasuriya UB, Karaca K, Audonnet JC, Yao J, He L, Nordgren R, Monaco F, Savini G, Gardner IA, Maclachlan NJ (2007) Recombinant canarypox virus vaccine co-expressing genes encoding the VP2 and VP5 outer capsid proteins of bluetongue virus induces high level protection in sheep. Vaccine 25:672-678
3. Hassan SS, Roy P (1999) Expression and functional characterization of bluetongue virus VP2 protein: role in cell entry. J Virol 73:9832-9842
4. Kouzmitcheva GA, Petrenko VA, Smith GP (2001) Identifying diagnostic peptides for lyme disease through epitope discovery. Clin Diagn Lab Immun 8:150-160
5. Langeveld JP, Casal JI, Osterhaus AD, Cortes E, de Swart R, Vela C, Dalsgaard K, Puijk WC, Schaaper WM, Meloen RH (1994) First peptide vaccine providing protection against viral infection in the target animal: studies of canine parvovirus in dogs. J Virol 68:4506-4513
6. Peng WP, Hou Q, Xia ZH, Chen D, Li N, Sun Y, Qiu HJ (2008) Identification of a conserved linear B-cell epitope at the N-terminus of the E2 glycoprotein of classical swine fever virus by phage-displayed random peptide library. Virus Res 135:267-272
7. Purse BV, Mellor PS, Rogers DJ, Samuel AR, Mertens PP, Baylis M (2005) Climate change and the recent emergence of bluetongue in Europe. Nat Rev Microbiol 3:171-181
8. Ratinier M, Caporale M, Golder M, Franzoni G, Allan K, Nunes SF, Armezzani A, Bayoumy A, Rixon F, Shaw A, Palmarini M (2011) Identification and characterization of a novel non-structural protein of bluetongue virus. PLoS Pathog 7:e1002477
9. Roy P (1989) Bluetongue virus genetics and genome structure. Virus Res 13:179-206
10. Roy P (1992) Bluetongue virus proteins. J Gen Virol 73:3051-3064
11. Roy P (2005) Bluetongue virus proteins and particles and their role in virus entry, assembly, and release. Adv Virus Res 64:69-123
12. Roy P (2008) Bluetongue virus: dissection of the polymerase complex. J Gen Virol 89:1789-1804
13. Sukupolvi-Petty S, Austin SK, Engle M, Brien JD, Dowd KA, Williams KL, Johnson S, Rico-Hesse R, Harris E, Pierson TC, Fremont DH, Diamond MS (2010) Structure and function analysis of therapeutic monoclonal antibodies against dengue virus type 2. J Virol 84:9227-9239
14. Sun EC, Zhao J, Liu NH, Yang T, Ma JN, Geng HW, Wang LF, Qin YL, Bu ZG, Yang YH, Lunt RA, Wu DL (2012) Comprehensive mapping of West Nile virus (WNV)- and Japanese encephalitis virus serocomplex-specific linear B-cell epitopes from WNV non-structural protein 1. J Gen Virol 93:50-60
15. Tian Y, Chen W, Yang Y, Xu X, Zhang J, Wang J, Xiao L, Chen Z (2012) Identification of B cell epitopes of dengue virus 2 NS3 protein by monoclonal antibody. Appl Microbiol Biotechnol. doi: 10.1007/s00253-012-4419-z
16. Wang W-S, Sun E-C, Liu N-H, Yang T, Xu Q-Y, Qin Y-L, Zhao J, Feng Y-F, Li J-P, Wei P, Zhang C-Y, Wu D-L (2012a) Monoclonal antibodies against VP7 of bluetongue virus. Hybridoma (Larchmt) 31:469-472
17. Wang W-S, Sun E-C, Liu N-H, Yang T, Xu Q-Y, Qin Y-L, Zhao J, Feng Y-F, Li J-P, Wei P, Zhang C-Y, Wu D-L (2012b) Identification of three novel linear B-cell epitopes on the VP5 protein of BTV16. Vet Microbiol. doi: 10.1016/j.vetmic.2012.11.042
18. Wilson AJ, Mellor PS (2009) Bluetongue in Europe: past, present and future. Philosophical transactions of the Royal Society of London. Series B, Biological sciences 364:2669-2681
19. Yang T, Liu N, Xu Q, Sun E, Qin Y, Zhao J, Wu D (2011) Complete genomic sequence of bluetongue virus serotype 16 from China. J Virol 85:13472
20. Zhang X, Boyce M, Bhattacharya B, Schein S, Roy P, Zhou ZH (2010) Bluetongue virus coat protein VP2 contains sialic acid-binding domains, and VP5 resembles enveloped virus fusion proteins. P Natl Acad Sci USA 107:6292-6297 Interest Research Institutes Research Institutes