Identification of three novel linear B-cell epitopes on the VP5 protein of BTV16

Veterinary Microbiology

Volumn 162, Issue 2-4, 2013, Pages 631-642

  Wang, Wen Shi ^a   Sun, En cheng ^a   Liu, Ni Hong ^a   Yang, Tao ^a   Xu, Qing Yuan ^a   Qin, Yong Li ^a   Zhao, Jing ^a   Feng, Yu Fei ^a   Li, Jun Ping ^a   Wei, Peng ^a   Zhang, Cui Yun ^a   Wu, Dong lai ^a  

^a INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Bluetongue virus; Epitopes; Monoclonal antibodies; VP5 protein

Indexed keywords

AMINO ACID; B LYMPHOCYTE ANTIGEN; EPITOPE; MALTOSE BINDING PROTEIN; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY 1H7; MONOCLONAL ANTIBODY 2B10; MONOCLONAL ANTIBODY 3B11; MONOCLONAL ANTIBODY 3G9; MONOCLONAL ANTIBODY 4A6; POLYPEPTIDE; PROTEIN VP5; RECOMBINANT PROTEIN; RECOMBINANT VP5 PROTEIN; UNCLASSIFIED DRUG; VIRUS PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANTIGENICITY; ARTICLE; BLUETONGUE ORBIVIRUS; BLUETONGUE ORBIVIRUS 16; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DRUG SYNTHESIS; EPITOPE MAPPING; IMMUNOREACTIVITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN MOTIF; SEQUENCE ALIGNMENT; VIRUS STRAIN;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODIES, VIRAL; ANTIBODY SPECIFICITY; CAPSID PROTEINS; CELL LINE, TUMOR; EPITOPE MAPPING; EPITOPES, B-LYMPHOCYTE; FEMALE; GUINEA PIGS; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; MULTIPLE MYELOMA; PEPTIDE FRAGMENTS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

BLUETONGUE VIRUS;

EID: 84872488248     PISSN: 03781135     EISSN: 18732542     Source Type: Journal    
DOI: 10.1016/j.vetmic.2012.11.042     Document Type: Article

References (21)

1. Calvo-Pinilla E., Nieto J.M., Ortego J. Experimental oral infection of bluetongue virus serotype 8 in type I interferon receptor-deficient mice. J. Gen. Virol. 2010, 91:2821-2825.
2. Forzan M., Wirblich C., Roy P. A capsid protein of nonenveloped Bluetongue virus exhibits membrane fusion activity. Proc. Natl. Acad. Sci. U. S. A. 2004, 101:2100-2105.
3. French T.J., Marshall J.J., Roy P. Assembly of double-shelled, viruslike particles of bluetongue virus by the simultaneous expression of four structural proteins. J. Virol. 1990, 64:5695-5700.
4. Hassan S.H., Wirblich C., Forzan M., Roy P. Expression and functional characterization of bluetongue virus VP5 protein: role in cellular permeabilization. J. Virol. 2001, 75:8356-8367.
5. Hewat E.A., Booth T.F., Roy P. Structure of bluetongue virus particles by cryoelectron microscopy. J. Struct. Biol. 1992, 109:61-69.
6. Huismans H., Van Der Walt N.T., Cloete M., Erasmus B.J. Isolation of a capsid protein of bluetongue virus that induces a protective immune response in sheep. Virology 1987, 157:172-179.
7. Kahlon J., Sugiyama K., Roy P. Molecular basis of bluetongue virus neutralization. J. Virol. 1983, 48:627-632.
8. Kohler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 1975, 256:495-497.
9. Lobato Z.I., Coupar B.E., Gray C.P., Lunt R., Andrew M.E. Antibody responses and protective immunity to recombinant vaccinia virus-expressed bluetongue virus antigens. Vet. Immunol. Immunopathol. 1997, 59:293-309.
10. Ma G., Eschbaumer M., Said A., Hoffmann B., Beer M., Osterrieder N. An equine herpesvirus type 1 (EHV-1) expressing VP2 and VP5 of serotype 8 bluetongue virus (BTV-8) induces protection in a murine infection model. PLoS ONE 2012, 7:e34425.
11. Mellor P.S. The replication of bluetongue virus in Culicoides vectors. Curr. Top. Microbiol. Immunol. 1990, 162:143-161.
12. Mertens P.P., Burroughs J.N., Anderson J. Purification and properties of virus particles, infectious subviral particles, and cores of bluetongue virus serotypes 1 and 4. Virology 1987, 157:375-386.
13. Mertens P.P., Pedley S., Cowley J., Burroughs J.N., Corteyn A.H., Jeggo M.H., Jennings D.M., Gorman B.M. Analysis of the roles of bluetongue virus outer capsid proteins VP2 and VP5 in determination of virus serotype. Virology 1989, 170:561-565.
14. Nason E.L., Rothagel R., Mukherjee S.K., Kar A.K., Forzan M., Prasad B.V., Roy P. Interactions between the inner and outer capsids of bluetongue virus. J. Virol. 2004, 78:8059-8067.
15. Roy P., Urakawa T., Van Dijk A.A., Erasmus B.J. Recombinant virus vaccine for bluetongue disease in sheep. J. Virol. 1990, 64:1998-2003.
16. Singh K.P., Maan S., Samuel A.R., Rao S., Meyer A.J., Mertens P.P. Phylogenetic analysis of bluetongue virus genome segment 6 (encoding VP5) from different serotypes. Vet. Ital. 2004, 40:479-483.
17. Sun E.C., Zhao J., Liu N.H., Yang T., Ma J.N., Geng H.W., Wang L.F., Qin Y.L., Bu Z.G., Yang Y.H., Lunt R.A., Wu D.L. Comprehensive mapping of West Nile virus (WNV)- and Japanese encephalitis virus serocomplex-specific linear B-cell epitopes from WNV non-structural protein 1. J. Gen. Virol. 2012, 93:50-60.
18. Sun E.C., Zhao J., Yang T., Liu N.H., Geng H.W., Qin Y.L., Wang L.F., Bu Z.G., Yang Y.H., Lunt R.A., Wu D.L. Identification of a conserved JEV serocomplex B-cell epitope by screening a phage-display peptide library with a mAb generated against West Nile virus capsid protein. Virol. J. 2011, 8:100.
19. Wang L.F., Du Plessis D.H., White J.R., Hyatt A.D., Eaton B.T. Use of a gene-targeted phage display random epitope library to map an antigenic determinant on the bluetongue virus outer capsid protein VP5. J. Immunol. Methods 1995, 178:1-12.
20. Yang Y.Y., Johnson T.M., Mecham J.O., Tam J.P., Li J.K. Epitopic mapping of linear and conformation-dependent antigenic determinants on GP5 of five U.S. bluetongue viruses. Virology 1992, 188:530-536.
21. Zhang X., Boyce M., Bhattacharya B., Schein S., Roy P., Zhou Z.H. Bluetongue virus coat protein VP2 contains sialic acid-binding domains, and VP5 resembles enveloped virus fusion proteins. Proc. Natl. Acad. Sci. U. S. A. 2010, 107:6292-6297.