The outer membrane of gram-negative bacteria: Lipid A isolation and characterization

Methods in Molecular Biology

Volumn 966, Issue , 2013, Pages 239-258

  Hankins, Jessica V ^a   Madsen, James A ^a   Needham, Brittany D ^a   Brodbelt, Jennifer S ^a   Trent, M Stephen ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

Bligh Dyer; Collision induced dissociation; Lipid A isolation; Lipopolysaccharide; Mass spectrometry; Photodissociation (PD); Thin layer chromatography

Indexed keywords

LIPID A; OUTER MEMBRANE PROTEIN;

ARTICLE; CARBOHYDRATE ANALYSIS; CHEMISTRY; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; MOLECULAR GENETICS; THIN LAYER CHROMATOGRAPHY;

BACTERIAL OUTER MEMBRANE PROTEINS; CARBOHYDRATE SEQUENCE; CHROMATOGRAPHY, THIN LAYER; LIPID A; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA;

MLCS; MLOWN;

EID: 84879310913     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-245-2_15     Document Type: Article

References (26)

1. Trent MS et al (2006) Diversity of endotoxin and its impact on pathogenesis. J Endotoxin Res 12:205-223
2. Kim HM et al (2007) Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran. Cell 130:906-917
3. Raetz CR et al (2007) Lipid A modification systems in Gram-negative bacteria. Annu Rev Biochem 76:295-329
4. Yi EC, Hackett M (2000) Rapid isolation method for lipopolysaccharide and lipid A from gram-negative bacteria. Analyst 125:651-656
5. Darveau RP, Hancock RE (1983) Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains. J Bacteriol 155:831-838
6. Galanos C, Luderitz O, Westphal O (1969) A new method for the extraction of R lipopolysaccharides. Eur J Biochem 9:245-249
7. Li J et al (1998) Development of an on-line preconcentration method for the analysis of pathogenic lipopolysaccharides using capillary electrophoresis-electrospray mass spectrometry. Application to small colony isolates. J Chromatogr 817:325-336
8. Westphal O, Jann K (1965) Bacterial lipopolysaccharide: extraction with phenol-water and further application of the procedure. Meth Carbohydr Chem 5:83-91
9. Caroff M et al (1991) Analysis of unmodified endotoxin preparations by 252Cf plasma desorption mass spectrometry: determination of molecular masses of the constituent native lipopolysaccharides. J Biol Chem 266: 18543-18549
10. Odegaard TJ et al (1997) Shortened hydroxyacyl chains on lipid A of Escherichia coli cells expressing a foreign UDP-N-acetylglucosamine O-acyltransferase. J Biol Chem 272:19688-19696
11. Banoub JH et al (2010) Structural investigation of bacterial lipopolysaccharides by mass spectrometry and tandem mass spectrometry. Mass Spectrom Rev 29:606-650
12. Madsen JA et al (2011) IR and UV photodissociation as analytical tools for characterizing Lipid A structures. Anal Chem (Washington, DC, US) 83:5107-5113
13. Hankins JV et al (2011) Elucidation of a novel Vibrio cholerae lipid A secondary hydroxy-acyltransferase and its role in innate immune recognition. Mol Microbiol 81:1313-1329
14. Chan S, Reinhold VN (1994) Detailed structural characterization of lipid A: electrospray ionization coupled with tandem mass spectrometry. Anal Biochem 218:63-73
15. Boue SM, Cole RB (2000) Con firmation of the structure of lipid A from Enterobacter agglomerans by electrospray ionization tandem mass spectrometry. J Mass Spectrom 35:361-368
16. Kussak A, Weintraub A (2002) Quadrupole ion-trap mass spectrometry to locate fatty acids on lipid A from Gram-negative bacteria. Anal Biochem 307:131-137
17. El-Aneed A, Banoub J (2005) Elucidation of the molecular structure of lipid A isolated from both a rough mutant and a wild strain of Aeromonas salmonicida lipopolysaccharides using electrospray ionization quadrupole timeof-flight tandem mass spectrometry. Rapid Commun Mass Spectrom 19:1683-1695
18. Murphy RC et al (2005) Mass spectrometry advances in lipidomica: collision-induced decomposition of Kdo 2 -lipid A. Prostaglandins Other Lipid Mediat 77:131-140
19. Lee C-S et al (2004) Structural analysis of lipid A from Escherichia coli O157:H7:K- using thin-layer chromatography and ion-trap mass spectrometry. J Mass Spectrom 39:514-525
20. Wang Z, Li J, Altman E (2006) Structural characterization of the lipid A region of Aeromonas salmonicida subsp. salmonicida lipopolysaccharide. Carbohydr Res 341:2816-2825
21. Mikhail I et al (2005) Structural characterization of lipid A from nontypeable and type f Haemophilus in fluenzae: variability of fatty acid substitution. Anal Biochem 340:303-316
22. Madalinski G et al (2006) Gram-negative bacterial lipid A analysis by negative electrospray ion trap mass spectrometry: stepwise dissociations of deprotonated species under low energy CID conditions. Int J Mass Spectrom 249/250:77-92
23. Silipo A et al (2008) Structural characterizations of lipids A by MS/MS of doubly charged ions on a hybrid linear ion trap/ orbitrap mass spectrometer. J Mass Spectrom 43:478-484
24. Jones JW et al (2008) Determination of pyrophosphorylated forms of lipid A in gramnegative bacteria using a multivaried mass spectrometric approach. Proc Natl Acad Sci U S A 105:12742-12747
25. Jones JW et al (2010) Comprehensive structure characterization of lipid A extracted from Yersinia pestis for determination of its phosphorylation con figuration. J Am Soc Mass Spectrom 21:785-799
26. Hankins JV, Trent MS (2009) Secondary acylation of Vibrio cholerae lipopolysaccharide requires phosphorylation of Kdo. J Biol Chem 284:25804-25812